Methods, systems, and computer-readable media for optimizing and enhancing delivery of application virtualization layers to client computing devices are described herein. In various embodiments, an application virtualization layer optimization service may identify a first and a second application virtualization layer to be delivered to one or more client computing devices. Each application virtualization layer may represent a package of one or more applications. A layer analysis service may analyze the first and second application virtualization layers to determine conflicts between the layers, using predetermined conflict analysis rules, and generate an actionable conflict resolution report based on the analysis. Based on the actionable conflict resolution report, the application virtualization layer optimization service may resolve conflicts between the first and second application virtualization layers, order the first and application virtualization layers, and deliver the ordered layers to the one or more client computing devices.