An over-voltage protection circuit for executing an over-voltage protection for a LED module of an electronic device. The over-voltage protection circuit includes a control unit and an over-voltage detection module, a trigger module and an adjustment module. The LED module includes a positive input port. The over-voltage detection module detects a voltage of the positive input port of the LED module and produce a detection voltage proportional to the voltage of the positive input port. The control unit controls the LED module to work or do not work according to the detection voltage. The trigger module produces corresponding trigger signals according to a 2D signal or a 3D signal output by a 2D/3D signal port. The adjustment module adjusts the proportion between the detection voltage and the voltage of the positive input port according to the trigger signal.