A process for preparing decellularized isolated bone tissue samples, comprises the steps of a) providing an isolated bone tissue sample, b) treating said sample by immersions in: a solution comprising at least one chelating agent, a solution comprising at least one anionic detergent, a solution comprising at least one non-ionic detergent, a solution comprising at least one enzyme, a solution comprising at least one antibiotic. Decellularized bone tissue samples as obtainable by a process according to the invention can be used as grafts.