An amplifying circuit includes a reference voltage generating circuit, a common-mode voltage conversion circuit, a common-mode negative feedback circuit, and an amplifying sub-circuit. The reference voltage generating circuit generates a first reference voltage, a second reference voltage, and a reference common-mode voltage according to a post-stage common-mode voltage. The common-mode voltage conversion circuit converts the pre-stage output differential signal into a differential input signal according to the reference common-mode voltage. The common-mode negative feedback circuit generates a control voltage to quickly establish a common-mode negative feedback of the amplifying sub-circuit, wherein the first reference voltage and the second reference voltage are used to cancel a baseline signal of the pre-stage output differential signal. The amplifying circuit can eliminate the baseline signal, convert the common-mode voltage and quickly establish the common-mode negative feedback.