An array substrate and a manufacturing method thereof, and a display panel including the array substrate and a driving method thereof are provided. Each of the sub-pixel units of the array substrate includes a first thin film transistor and a second thin film transistor; the first thin film transistor includes a first gate electrode, a first source electrode and a first drain electrode; the second thin film transistor includes a second gate electrode, a second source electrode and a second drain electrode; each of the sub-pixel unit further includes a first pixel electrode electrically connected to the first drain electrode, a second pixel electrode electrically connected to the second drain electrode; and the first pixel electrode and the second pixel electrode are disposed in different layers and insulated with each other.