- Patent Title: Cas9 plasmid, genome editing system and method of Escherichia coli
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Application No.: US15495580Application Date: 2017-04-24
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Publication No.: US09988637B2Publication Date: 2018-06-05
- Inventor: Yu Chen Hu , Mu-En Chung , I-Hsin Yeh , Hung Li , Li-Yu Sung
- Applicant: NATIONAL TSING HUA UNIVERSITY , CHANG CHUN PLASTICS CO., LTD , CHANG CHUN PETROCHEMICAL CO., LTD
- Applicant Address: TW Hsinchu TW Taipei TW Taipei
- Assignee: NATIONAL TSING HUA UNIVERISTY,CHANG CHUN PLASTICS CO., LTD,CHANG CHUN PETROCHEMICAL CO., LTD
- Current Assignee: NATIONAL TSING HUA UNIVERISTY,CHANG CHUN PLASTICS CO., LTD,CHANG CHUN PETROCHEMICAL CO., LTD
- Current Assignee Address: TW Hsinchu TW Taipei TW Taipei
- Agency: Li & Cai Intellectual Property (USA) Office
- Main IPC: C12N15/73
- IPC: C12N15/73 ; C12N9/22 ; C12N15/11 ; C12N15/72 ; C12N15/90 ; C07K14/245
Abstract:
A Cas9 expression plasmid, a genome editing system and a genome editing method for Escherichia coli are provided. The Cas9 expression plasmid includes a tracrRNA sequence, a Cas9 gene and a chloramphenicol resistance gene (CmR). The Cas9 expression plasmid is applied to CRISPR/Cas-coupled λ-red recombineering system for editing genomes of E. coli with high efficiency.
Public/Granted literature
- US20170226522A1 CAS9 PLASMID, GENOME EDITING SYSTEM AND METHOD OF ESCHERICHIA COLI Public/Granted day:2017-08-10
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