Invention Grant
- Patent Title: Vectors with modified initiation codon for the translation of AAV-REP78 useful for production of AAV
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Application No.: US15424560Application Date: 2017-02-03
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Publication No.: US09988645B2Publication Date: 2018-06-05
- Inventor: Wilhelmus Theodorus Johannes Maria Christiaan Hermens , Saskia Jacoba Petronella Haast , Dennis Johan Biesmans , Andrew Christian Bakker
- Applicant: uniQure IP B.V.
- Applicant Address: NL Amsterdam
- Assignee: uniQure IP B.V.
- Current Assignee: uniQure IP B.V.
- Current Assignee Address: NL Amsterdam
- Agency: Foley & Lardner LLP
- Agent Gilberto M. Villacorta; Sunit Talapatra
- Priority: EP06115804 20060621
- Main IPC: C12N7/00
- IPC: C12N7/00 ; C12N15/86 ; C07K14/005 ; C12N15/864 ; C12N5/10

Abstract:
The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AA V) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
Public/Granted literature
- US20170145440A1 VECTORS WITH MODIFIED INITIATION CODON FOR THE TRANSLATION OF AAV-REP78 USEFUL FOR PRODUCTION OF AAV Public/Granted day:2017-05-25
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