An example target acquisition method includes obtaining, according to a global feature of each video frame of a plurality of video frames, a target pre-estimated position of each scale in the video frame; clustering the target pre-estimated position in each video frame to obtain a corresponding target candidate region; and determining a target actual region in the video frame according to all the target candidate regions in each video frame in combination with confidence levels of the target candidate regions and corresponding scale processing. The techniques of the present disclosure quickly and effectively acquire one or multiple targets, and, more particularly, achieve accurately distinguishing and acquiring the multiple targets.