The present invention provides a display backplane and a manufacturing method thereof, as well as a display device. The display backplane comprising: a substrate; an array of organic light emitting elements and an array of transistors for driving and controlling the array of organic light emitting elements formed on the substrate; and a heat insulation layer formed between the array of organic light emitting elements and the array of transistors, wherein the heat insulation layer is provided with a heat insulation layer via hole, through which the array of transistors is connected with the array of organic light emitting elements. The display backplane provided in the present invention can reduce the conduction of heat generated by the array of organic light emitting elements during lighting to the array of transistors, thus avoiding problems caused thereby such as uneven display.