公开(公告)号：WO0066985A9

公开(公告)日：2002-02-14

申请号：PCT/EP0003890

申请日：2000-04-29

Applicant: EVOTEC BIOSYSTEMS AG ,  PALO KAUKO ,  KASK PETE

Inventor： PALO KAUKO ,  KASK PETE

IPC: G01J3/443 ,  G01J3/28 ,  G01J3/44 ,  G01J3/447 ,  G01J3/457 ,  G01N15/02 ,  G01N21/64

CPC classification number: G01N21/6458 ,  G01J3/4406 ,  G01J3/457 ,  G01J2003/2866 ,  G01N21/6408 ,  G01N2021/6421 ,  G01N2021/6463

Abstract: A method for characterizing fluorescent molecules or other particles in samples comprising the steps of: a) monitoring fluctuating intensity of fluorescence emitted by the molecules or other particles in at least one measurement volume of a non-uniform spatial brightness profile by measuring numbers of photon counts in primary time intervals by a single or more photon detectors, b) determining at least one distribution of numbers of photon counts, P(n), from the measured numbers of photon counts, c) determining physical quantities characteristic to said particles by fitting the distribution of numbers of photon counts P(n), wherein the fitting procedure involves calculation of a theoretical distribution function of the number of photon counts P(n) through its generating function, defined as (I).

Abstract translation: 用于表征样品中的荧光分子或其他颗粒的方法，包括以下步骤：a）通过测量光子计数来监测在非均匀空间亮度分布的至少一个测量体积中由分子或其它颗粒发射的荧光的波动强度 通过单个或多个光子检测器在主要时间间隔中，b）从所测量的光子计数中确定光子计数数目P（n）的至少一个分布，c）通过拟合所述粒子的特征来确定所述粒子的特征量 光子计数P（n）的分布，其中拟合过程包括通过其生成函数（i）计算光子计数P（n）的数量的理论分布函数。