公开(公告)号：KR1020080008668A

公开(公告)日：2008-01-24

申请号：KR1020060068178

申请日：2006-07-20

Applicant: 재단법인서울대학교산학협력재단

Inventor： 현택환 ,  이인수

IPC: B82B1/00 ,  B82Y5/00

CPC classification number: C07K1/22 ,  B82Y5/00 ,  B82Y25/00 ,  H01F1/0054

Abstract: A method for selective binding, separation or purification of specific proteins using magnetic nanoparticles is provided to bind the nanoparticles selectively to a specific amino acid-tagged protein and separate the protein effectively. A method for selective binding, separation or purification of specific proteins includes the steps of: binding magnetic nanoparticles of 1-1000nm in size, which consists of Group I transition metals (iron, manganese, nickel, cobalt, zinc, etc) or transition metal ions, to specific amino acid-tagged proteins in In-Vivo blends; separating the proteins bound with the nanoparticles from the In-Vivo blends by an externally applied magnetic field; and separating the specific proteins from the separated nanoparticles.

Abstract translation: 提供了使用磁性纳米颗粒选择性结合，分离或纯化特定蛋白质的方法，以将纳米粒子选择性地结合到特定的氨基酸标记的蛋白质上并有效分离蛋白质。 选择性结合，分离或纯化特定蛋白质的方法包括以下步骤：结合尺寸为1-1000nm的磁性纳米颗粒，其由I族过渡金属（铁，锰，镍，钴，锌等）或过渡金属 离子，在体内共混物中的特定氨基酸标记的蛋白质; 通过外部施加的磁场将与纳米颗粒结合的蛋白与体内共混物分离; 并从分离的纳米颗粒中分离特定的蛋白质。