-
公开(公告)号：KR1020080068352A
公开(公告)日：2008-07-23
申请号：KR1020070006015
申请日：2007-01-19
Applicant: 재단법인서울대학교산학협력재단
CPC classification number: G01N33/5058 , A61K35/28 , A61K2035/124 , C12N5/0663 , G01N33/5082 , G01N2800/285
Abstract: A method for screening materials with neuronal regeneration effect using a tissue fragment of the spinal cord is provided to be usefully used to develop a therapeutic agent of neuronal diseases. A method for screening neuronal regeneration materials comprises the steps of: (a) culturing a tissue fragment obtained from the spinal cord; (b) treating the tissue fragment under the culturing with a toxic compound to damage the tissue fragment; (c) treating the damaged tissue fragment with a candidate material expected to have the neuronal regeneration effect; and (d) screening the candidate material showing significant neuronal regeneration effect by comparing the result with a negative control group not-treated with the candidate material, wherein the candidate material is a compound, a growth factor, cytokine, a mesenchymal stem cell derived factor, an extracellular matrix protein, a protein involved with growth of axon, or a factor involved with intracellular signal transduction and the toxic compound is lysolecithin inducing demyelination of the axon of the spinal cord. Further, the damage is demyelination.
Abstract translation: 提供使用脊髓的组织片段筛选具有神经元再生作用的材料的方法，以有效地用于开发神经元疾病的治疗剂。 用于筛选神经元再生材料的方法包括以下步骤：（a）培养从脊髓获得的组织片段; （b）用毒性化合物处理组织片段，以损伤组织片段; （c）用预期具有神经元再生作用的候选物质治疗损伤的组织片段; 和（d）通过将未与所述候选物质未处理的阴性对照组比较，筛选显示出显着神经元再生作用的候选物质，其中所述候选物质是化合物，生长因子，细胞因子，间充质干细胞衍生因子 ，细胞外基质蛋白，涉及轴突生长的蛋白质或涉及细胞内信号转导的因子和毒性化合物是溶血卵磷脂诱导脊髓轴突的脱髓鞘。 此外，损伤是脱髓鞘。
-
公开(公告)号：KR100890992B1
公开(公告)日：2009-03-27
申请号：KR1020070006014
申请日：2007-01-19
Applicant: 재단법인서울대학교산학협력재단
Abstract: 본 발명은 조성물이 최적화된 배지에서 배양함으로써 골수 내에 존재하는 중간엽 줄기세포를 성숙한 신경세포로 분화시키는 방법에 관한 것으로, 구체적으로 최적화된 배지에서 배양함으로써 골수내에 존재하는 중간엽 줄기세포를 성숙한 신경세포로 분화시키는 방법에 관한 것이다. 본 발명의 전-유도 방법 및 부틸 하이드록시아니솔, 포스콜린 및 VPA를 포함하는 신경유도배지(neuronal induction media, NIM)를 사용하는 방법으로 중간엽 줄기세포를 신경세포 또는 운동신경세포로 효과적으로 분화시켜, 신경 질환의 치료를 위한 세포 치료제로 사용할 수 있다.
중간엽 줄기세포, 신경세포로의 분화, 운동신경세포-
公开(公告)号：KR1020080068351A
公开(公告)日：2008-07-23
申请号：KR1020070006014
申请日：2007-01-19
Applicant: 재단법인서울대학교산학협력재단
Abstract: A method for inducing differentiation of mesenchymal stem cells using pre-inductions and neuronal induction media(NIM) supplemented with butylated hydroxyanisole, forskolin and VPA is provided to differentiate the mesenchymal stem cells into neurons or motor neurons effectively, thereby being used as a cell therapeutic agent of neurodegenerative diseases. A method for inducing differentiation of mesenchymal stem cells into neurons comprises the steps of: (a) performing pre-induction of mesenchymal stem cells twice; and (b) inducing differentiation of the pre-differentiated mesenchymal stem cells of the step(a) in a neuronal induction medium containing butylated hydroxyanisole(BHA), forskolin and valproic acid (VPA) for 2-8 hours, wherein the content of beta-mercaptoethanol added for the second pre-induction is increased 1.5-2 times from the content added for the first pre-induction. The second pre-induction time is reduced to 1/4-1/8 of the first pre-induction time and the neuronal induction medium contains 100-200 muM of BHA, 9-11 muM of forskolin and 1.5-2.5 muM of VPA.
Abstract translation: 提供使用预诱导和补充有丁基化羟基茴香醚，毛喉素和VPA的神经元诱导培养基（NIM）诱导间充质干细胞分化的方法，以有效地将间充质干细胞分化为神经元或运动神经元，从而用作细胞治疗 神经变性疾病药物。 诱导间充质干细胞分化为神经元的方法包括以下步骤：（a）进行间充质干细胞的预诱导两次; （b）在含有丁基化羟基苯甲醚（BHA），毛喉素和丙戊酸（VPA）的神经元诱导培养基中诱导步骤（a）的分化前间充质干细胞分化2-8小时，其中β 加入第二次预诱导的巯基乙醇从第一次预诱导加入的含量增加1.5-2倍。 第二次预诱导时间降低到第一次诱导前时间的1 / 4-1 / 8，神经元诱导培养基含有100-200μM的BHA，9-11μM的毛喉素和1.5-2.5μM的VPA。
-
-
Search Results
3
records
(took 0.019 seconds)
