公开(公告)号：KR1020040067638A

公开(公告)日：2004-07-30

申请号：KR1020030004853

申请日：2003-01-24

Applicant: 재단법인서울대학교산학협력재단

Inventor： 민병무 ,  허성주 ,  김철생

IPC: A61K6/00

CPC classification number: A61K6/08 ,  A61K6/0085 ,  A61K6/033

Abstract: PURPOSE: Provided is a surface-treated implant titanium which facilitates the adhesion of osteoblast to the bone-implant location as well as the adhesion of fibroblast to the gingival location, thereby exerting enhanced tissue healing effect. CONSTITUTION: A method of surface-treating the implant comprises the steps of: dipping implant titanium or titanium alloy in a hydroxyapatite paste, and drying it; heat-treating the implant titanium or titanium alloy at 800-900 deg.C for 1-2 hours under the atmosphere of argon or nitrogen gas; and dipping the heat-treated implant titanium or titanium alloy in a solution of type I collagen with a concentration of 0.005-0.5% or a fibronectin solution with a concentration of 0.0001-0.5% for 1-12 hours to thereby surface-coat it.

Abstract translation: 目的：提供了一种表面处理的植入物钛，其有助于成骨细胞与骨植入位置的粘附以及成纤维细胞与牙龈位置的粘附，从而发挥增强的组织愈合作用。 构成：对植入物进行表面处理的方法包括以下步骤：将钛或钛合金浸入羟基磷灰石膏中并干燥; 在氩气或氮气气氛下，在800-900摄氏度下对植入钛或钛合金进行热处理1-2小时; 并将热处理的植入物钛或钛合金浸渍在浓度为0.005-0.5％的I型胶原溶液中，或将浓度为0.0001-0.5％的纤连蛋白溶液浸渍1-12小时，由此进行表面包覆。

公开(公告)号：KR1020030087430A

公开(公告)日：2003-11-14

申请号：KR1020020025785

申请日：2002-05-10

Applicant: 재단법인서울대학교산학협력재단

Inventor： 장준혁 ,  정종평 ,  허성주

IPC: C07K7/06

CPC classification number: C07K7/06 ,  C01B21/076

Abstract: PURPOSE: An oligopeptide specifically binding to Ti metal is provided. The oligopeptide can be useful as a linker between the Ti metal and biomolecules. CONSTITUTION: An oligopeptide specifically binding to Ti metal is provided, wherein the oligopeptide has the amino acid sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 or SEQ ID NO: 7. The oligopeptide specifically binding to Ti metal is isolated by inserting random amino acid sequences into a vector pFLITRIX containing the active portion of thioredoxin; transforming Escherichia coli with the vector pFLITRIX; culturing the transformed E. coli in an ampicillin containing IMC selection medium; adding tryptophane into the medium and culturing it to induce FLITRIX peptide; binding the transformed E. coli to Ti metal fixed on a dish; selecting peptides specifically binding to Ti metal; and sequencing the selected peptides.

Abstract translation: 目的：提供特异性结合Ti金属的寡肽。 寡肽可用作Ti金属和生物分子之间的接头。 提供了特异性结合Ti金属的寡肽，其中寡肽具有SEQ ID NO：1，SEQ ID NO：2，SEQ ID NO：3，SEQ ID NO：4，SEQ ID NO：5的氨基酸序列 通过将随机氨基酸序列插入到含有硫氧还蛋白活性部分的载体pFLITRIX中来分离与Ti金属特异性结合的寡肽;将SEQ ID NO：6或SEQ ID NO： 用载体pFLITRIX转化大肠杆菌; 在含有氨苄青霉素的IMC选择培养基中培养转化的大肠杆菌; 将色氨酸加入到培养基中并培养以诱导FLITRIX肽; 将转化的大肠杆菌与固定在盘上的Ti金属结合; 选择与Ti金属特异性结合的肽; 并对所选择的肽进行测序。

公开(公告)号：KR100546081B1

公开(公告)日：2006-01-26

申请号：KR1020030004853

申请日：2003-01-24

Applicant: 재단법인서울대학교산학협력재단

Inventor： 민병무 ,  허성주 ,  김철생

IPC: A61K6/00

Abstract: 본 발명은 단백질을 이용한 임플란트용 티타늄의 표면처리 방법에 관한 것으로, 보다 상세하게는 티타늄 또는 티타늄 합금을 수산화아파타이트 존재하에 열처리하고, 그 후 상기 열처리된 티타늄 또는 티타늄 합금을 타입 I 콜라젠 또는 피브로넥틴으로 표면처리하는 것으로 이루어진 표면처리 방법에 관한 것이다. 본 발명의 방법에 의해 표면처리된 임플란트용 티타늄 또는 티타늄 합금은 뼈에 매식되는 부위에서 골모세포(osteoblast)의 부착을 촉진시키고, 치은(gingiva)에 위치하는 부위에서 섬유모세포(fibroblast)의 부착을 촉진시켜 더욱 향상된 조직 치유 효과를 나타낸다.
임플란트, 표면처리방법, 티타늄, 타입 I 콜라젠, 피브로넥틴.

公开(公告)号：KR1020040014006A

公开(公告)日：2004-02-14

申请号：KR1020020047170

申请日：2002-08-09

Applicant: 재단법인서울대학교산학협력재단

Inventor： 장준혁 ,  정종평 ,  허성주 ,  김현이

IPC: C07K7/08

CPC classification number: C07K7/08 ,  C07K5/0806 ,  C07K5/10 ,  C07K7/06

Abstract: PURPOSE: Oligosaccharides specifically binding to hydroxyapatite are provided, which oligosaccharides are useful as linkers between biomolecules for increasing the affinity of hydroxyapatite to human body materials. CONSTITUTION: Oligosaccharides specifically binding to hydroxyapatite are provided, wherein the oligosaccharides have the amino acid sequence selected from the SEQ ID NO: 1 to SEQ ID NO: 17; the oligosaccharides are selected by screening peptides specifically binding to hydroxyapatite using peptide library; and the hydroxyapatite has similar chemical components and structure to the human bone.

Abstract translation: 目的：提供与羟基磷灰石特异性结合的寡糖，这些寡糖可用作生物分子之间的接头，用于增加羟基磷灰石与人体材料的亲和力。 提供：提供与羟基磷灰石特异性结合的寡糖，其中寡糖具有选自SEQ ID NO：1至SEQ ID NO：17的氨基酸序列; 通过使用肽文库筛选特异性结合羟基磷灰石的肽来选择寡糖; 羟基磷灰石具有与人骨相似的化学成分和结构。