公开(公告)号：KR100968640B1

公开(公告)日：2010-07-06

申请号：KR1020070100670

申请日：2007-10-06

Applicant: 재단법인서울대학교산학협력재단

Inventor： 박상현 ,  허재영

IPC: G01N35/00 ,  G01N33/48 ,  G01N33/53 ,  G01N33/533

Abstract: 본 발명은 단일세포의 신호분석방법에 관한 것으로서, 보다 상세하게는 단일세포 신호분석용 효모 형광균주를 제조하는 단계; 및 상기 형광 균주의 단일 세포로부터 신호 반응으로 단일세포를 분석하는 단계를 포함하는, 단일세포의 신호분석방법에 관한 것이다. 본 발명의 단일세포의 신호분석 방법을 이용하면 실시간 반응분석을 통하여 세포의 신호 조절 기작을 이해할 수 있다.
신호분석, 형광 단백질, 나노환경, 효모 형광균주

公开(公告)号：KR1020090035400A

公开(公告)日：2009-04-09

申请号：KR1020070100669

申请日：2007-10-06

Applicant: 재단법인서울대학교산학협력재단

Inventor： 박상현 ,  허재영

IPC: C12N15/04 ,  C12N15/00 ,  C12N1/16

Abstract: Provided is a method for preparing fluorescent yeast strain containing a gene which codes fluorescence protein and selection marker for single-cell analysis through a real-time reaction analysis. Fluorescent yeast strain for single-cell analysis comprises genes for coding fluorescence protein and selection marker. The gene for coding the fluorescence protein represents yEGFP, CFP, YFP, Tdimer2 or BFP. The selection marker is an essential amino acid synthetic gene of ura3, his3, trp1 or leu2. The genes for single-cell analysis indicate Fus1 for measuring mating signals or Gpd1 for measuring stress signals. The yeast strain is a yeast SO992 strain. A method for preparing the fluorescent yeast strain consists of the following steps of: preparing plasmid which codes fluorescence protein and selection marker; and performing PCR with a primer having the same 20-40 base pairs of base sequence as the plasmid and yeast chromosome.

Abstract translation: 提供了通过实时反应分析制备含有编码荧光蛋白的基因和用于单细胞分析的选择标记物的荧光酵母菌株的方法。 用于单细胞分析的荧光酵母菌株包括用于编码荧光蛋白和选择标记的基因。 用于编码荧光蛋白的基因代表yEGFP，CFP，YFP，Tdimer2或BFP。 选择标记是ura3，his3，trp1或leu2的必需氨基酸合成基因。 用于单细胞分析的基因表明用于测量交配信号的Fus1或用于测量应激信号的Gpd1。 酵母菌株是酵母SO992菌株。 制备荧光酵母菌株的方法包括以下步骤：制备编码荧光蛋白质和选择标记物的质粒; 并用具有与质粒和酵母染色体相同的20-40碱基对碱基序列​​的引物进行PCR。

公开(公告)号：KR1020090035401A

公开(公告)日：2009-04-09

申请号：KR1020070100670

申请日：2007-10-06

Applicant: 재단법인서울대학교산학협력재단

Inventor： 박상현 ,  허재영

IPC: G01N35/00 ,  G01N33/48 ,  G01N33/53 ,  G01N33/533

Abstract: A cell signaling analysis method of single-cell is provided to examine the signal control mechanism through real time reaction analysis, the activity, adaptation, and stochasticity of signaling. A cell signaling analysis method of single-cell comprises the steps of: manufacturing a yeast fluorescence strain for single cells signal analysis; analyzing single cells from the single cells of the fluorescence strain with signaling reaction; building the nano environment for the cell capture by using the strain; and catching the single cells from the nano environment.

Abstract translation: 提供单细胞的细胞信号分析方法，通过实时反应分析，信号的活动性，适应性和随机性检查信号控制机制。 单细胞的细胞信号分析方法包括以下步骤：制备用于单细胞信号分析的酵母荧光菌株; 用信号反应从荧光菌株的单细胞分析单细胞; 通过使用该菌株构建用于细胞捕获的纳米环境; 并从纳米环境中捕获单个细胞。