公开(公告)号：KR100901138B1

公开(公告)日：2009-06-09

申请号：KR1020080025021

申请日：2008-03-18

Applicant: 재단법인서울대학교산학협력재단 ,  주식회사 한국천연물사이언스

Inventor： 김영식 ,  임아랑 ,  이진성 ,  이대성 ,  송주은

IPC: A23L1/29 ,  A23L1/30

CPC classification number: A23L33/00 ,  A23L33/10 ,  A23V2002/00 ,  A23V2200/30 ,  A23V2250/204

Abstract: A method for preparing swine placenta extracts using lipase and protease and a health food comprising the same are provided to reduce contamination in a production process and to increase the content of a bioactive substance by adding lipase and protease to the swine placenta. A method for preparing swine placenta extracts comprises the following steps of: pulverizing frozen swine placenta 2-5mm in size; adding water and lipase to the pulverized swine placenta such that the swine placenta reacts with the lipase at 39-45°C for 5-10 hours; adding an emulsifier to the reaction liquid and agitating the liquid to collect a first supernate; adding the water and protease to the residue so that the residue reacts with the protease at 28-40°C for 12-20 hours; obtaining a second supernate from the residue; and mixing the first and second supernates.

Abstract translation: 提供了使用脂肪酶和蛋白酶制备猪胎盘提取物的方法和包含其的保健食品的方法，以减少生产过程中的污染并通过向猪胎盘加入脂肪酶和蛋白酶来增加生物活性物质的含量。 制备猪胎盘提取物的方法包括以下步骤：粉碎2-5mm的冷冻猪胎盘; 向粉碎的猪胎盘中加入水和脂肪酶，使得猪胎盘在39-45℃下与脂肪酶反应5-10小时; 向反应液中加入乳化剂并搅拌该液体以收集第一上清液; 向残留物中加入水和蛋白酶，使残留物在28-40℃下与蛋白酶反应12-20小时; 从残渣中获得第二上清液; 并混合第一和第二上清液。

公开(公告)号：KR1020090112528A

公开(公告)日：2009-10-28

申请号：KR1020080060815

申请日：2008-06-26

Applicant: 재단법인서울대학교산학협력재단 ,  주식회사 한국천연물사이언스

Inventor： 김영식 ,  임아랑 ,  하인진 ,  박유미 ,  정춘식 ,  송주은 ,  박영대

IPC: A61K35/32 ,  A61P1/00

Abstract: PURPOSE: A composition containing Cervi parvum cornu extract is provided to suppress gastric acid and colony formation of Helicobacter pylory. CONSTITUTION: A method for extracting Cervi parvum cornu comprises: a step of extracting powder of Cervi parvum cornu using ultra sonic wave with chloroform:methanol (2:1) at 30 minutes; a step of centrifuging the extract at 15 °C, 500 x g for 30 minutes to separate supernatant and precipitate; a step of collecting supernatant (organic layer); a step of concentrating at 0.5 bar to obtain 20 mg of oil soluble powder of Cervi parvum cornu; and a step of maintaining at -20 °C.

Abstract translation: 目的：提供含有Cervi parvum cornu提取物的组合物，以抑制幽门螺杆菌的胃酸和菌落形成。 构成：提取小白菜的方法包括：在30分钟时间内用氯仿：甲醇（2:1），用超声波提取小白菜的粉末的步骤; 将提取物在15℃，500×g离心30分钟以分离上清液和沉淀物的步骤; 收集上清液（有机层）的步骤; 浓缩0.5巴的步骤，得到20毫克的Cervi parvum cornu的油溶性粉末; 和保持在-20℃的步骤。

公开(公告)号：KR1020080010201A

公开(公告)日：2008-01-30

申请号：KR1020060070393

申请日：2006-07-26

Applicant: 재단법인서울대학교산학협력재단 ,  주식회사 글루칸

Inventor： 김영식 ,  심준수 ,  임아랑

IPC: A61K47/30

Abstract: A composition comprising the extract of Lophiomus setigerus or chondroitin sulfate isolated therefrom is provided to reduce the production costs by using inexpensive fish, inhibit infectious diseases derived from animals such as pig and cow, and improve effects of liver protection, lipid metabolism and anti-inflammation. A composition for protecting cartilage articulation comprises the extract of Lophiomus setigerus hard bone or cartilage containing chondroitin sulfate having an average molecular weight of 52,000 dalton, wherein the chondroitin sulfate contains 19-21 parts by weight of chondroitin, 61-63 parts by weight of chondroitin sulfate C, 15-17 parts by weight of chondroitin sulfate A, 0.5-1 parts by weight of 2,4-chondroitin sulfate and 0.1-0.3 parts by weight of 4,6-chondroitin sulfate. A method for preparing the extract of Lophiomus setigerus hard bone or cartilage comprises the steps of: (a) adding a decomposing agent and buffer solution to dried powder of Lophiomus setigerus hard bone or cartilage to decompose them; (b) precipitating proteins and nucleic acids produced in the reaction solution by using trichloroacetic acid and removing them; and (c) removed reaction solution and drying the protein and nucleic acid-removed reaction solution, wherein the decomposing agent is subtilisin. A method for isolating and purifying the chondroitin sulfate comprises the steps of: (i) dissolving the extract of Lophiomus setigerus hard bone or cartilage in distilled water and adding quaternary ammonium salt to form a precipitate composite; and (ii) solubilizing the precipitate composite with strong salt, and reprecipitating the solution with organic solvent.

Abstract translation: 提供了包含从其中分离出的Lophiomus setigerus或硫酸软骨素提取物的组合物，以通过使用便宜的鱼来减少生产成本，抑制源自动物如猪和牛的传染病，并改善肝脏保护，脂质代谢和抗炎的作用 。 用于保护软骨关节的组合物包括平均分子量为52,000道尔顿的硫酸软骨提取物或含有软骨素硫酸软骨的软骨，其中硫酸软骨素含有19-21重量份的软骨素，61-63重量份的软骨素 硫酸钙C，15-17重量份硫酸软骨素A，0.5-1重量份硫酸软骨素和0.1-0.3重量份的4,6-硫酸软骨素。 一种制备枸杞子硬骨或软骨提取物的方法，包括以下步骤：（a）将分解剂和缓冲溶液加入到枸杞子硬骨或软骨的干粉中以分解; （b）通过使用三氯乙酸将反应溶液中产生的蛋白质和核酸沉淀并除去; 和（c）除去反应溶液并干燥蛋白质和去除核酸的反应溶液，其中分解剂是枯草杆菌蛋白酶。 分离纯化硫酸软骨素的方法包括以下步骤：（i）将枸杞子的硬骨或软骨的提取物溶解在蒸馏水中，加入季铵盐形成沉淀复合物; 和（ii）用强盐溶解沉淀复合物，并用有机溶剂再沉淀该溶液。