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公开(公告)号：KR101068993B1
公开(公告)日：2011-09-30
申请号：KR1020090014690
申请日：2009-02-23
Applicant: 한국과학기술연구원
IPC: G01N33/74 , G01N33/573 , G01N30/74
Abstract: 본 발명은 측정된 뇨 내 스테로이드 호르몬들의 농도를 통계적인 방법으로 처리하여 각 스테로이드 호르몬의 농도뿐만 아니라 그들의 대사에 관여하는 효소들의 활성을 동시에 측정, 비교할 수 있는 분석 방법에 관한 것이다.
본 발명은 뇨 내 스테로이드 호르몬들의 농도 측정은 물론 대사에 관여하는 스테로이드 간의 비율을 나타냄으로서 총체적인 대사분포 경향을 파악할 수 있다. 따라서, 전립선 비대증과 같은 스테로이드 호르몬 대사와 관련 있는 내분비질환 및 생물학적 변화를 평가할 수 있는 방법으로 사용될 수 있으며, 호르몬 의존성의 다양한 질환에 대하여 그 응용범위를 확장할 수 있다.
뇨, 스테로이드, 효소활성, 전립선비대증-
公开(公告)号：KR101260453B1
公开(公告)日：2013-05-06
申请号：KR1020110095963
申请日：2011-09-22
Applicant: 한국과학기술연구원
Abstract: 본발명은생체시료내 스테로이드호르몬들의수산화대사에관여하는여러종류의시토크롬 P450 효소들의활성을질량분석기를이용하여동시에측정및 비교할수 있는분석방법에관한것이다. 본발명은소변과같은생체시료로부터약물복용에따른스테로이드호르몬대사와관련이있는여러종류의시토크롬 P450 효소들의활성도변화를그 대사산물들간의농도비율로나타냄으로써한 번의전처리와분석으로동시에측정할수 있다. 따라서특정약물복용에따른시토크롬 P450 효소활성도변화를통한약물효능평가및 대사기전을총체적으로평가할수 있고, 약물유전체기반의시토크롬 P450 효소분석과의교차검증(cross-validation)이가능한분석기술로도활용할수 있다. 또한, 이들의축척된정보들은신약개발및 약물대사뿐만아니라시토크롬 P450 효소들의활성과관련되는질환등의연구에있어서광범위하게적용될수 있다.
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Patent Agency Ranking
公开(公告)号：KR1020130032198A
公开(公告)日：2013-04-01
申请号：KR1020110095963
申请日：2011-09-22
Applicant: 한국과학기술연구원
Abstract: PURPOSE: A simultaneous assay method of multiplex cytochrome P450 enzyme activity is provided to simultaneously measure the activity change of various cytochrome P450 enzymes and to study diseases related to the cytochrome P450 enzyme activity. CONSTITUTION: A simultaneous assay method of multiplex cytochrome P450 enzyme activity comprises: a step of simultaneously extracting steroid hormone metabolic products including male hormones, female hormones, corpus luteum hormones, sterol, and adrenal cortical hormones from urine; a step of re-extracting the extracts using an organic solvent and performing trimethyl silyl derivatization; and a step of measuring the concentration of steroid hormone biometabolic products in the urine by gas chromatography-mass spectrometer. [Reference numerals] (AA) Before dosing a drug; (BB) After dosing a drug;
Abstract translation: 目的：提供多重细胞色素P450酶活性的同时测定方法，以同时测量各种细胞色素P450酶的活性变化，并研究与细胞色素P450酶活性相关的疾病。 构成：多重细胞色素P450酶活性的同时测定方法包括：从尿中同时提取包括雄性激素，雌激素，黄体激素，固醇和肾上腺皮质激素的类固醇激素代谢产物的步骤; 使用有机溶剂重新萃取提取物并进行三甲基甲硅烷基衍生化的步骤; 以及通过气相色谱 - 质谱仪测量尿中类固醇激素生物代谢产物浓度的步骤。 （AA）给药前给药; （BB）给药药物后;
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公开(公告)号：KR101200423B1
公开(公告)日：2012-11-12
申请号：KR1020110047673
申请日：2011-05-20
Applicant: 한국과학기술연구원
IPC: G01N30/72 , G01N30/02 , G01N33/74 , G01N33/487
Abstract: PURPOSE: A high sensitive analyzing method of estrogen metabolites in urine and blood obtained from postmenopausal women is provided to verify the overall distribution of estrogen metabolism and to be used for overcoming diseases related to estrogen metabolism. CONSTITUTION: A high sensitive analyzing method of estrogen metabolites in urine and blood obtained from postmenopausal women includes the following steps: estrogen is simultaneously extracted from urine and blood obtained from postmenopausal women and is hydrolyzed; the hydrolyzed product is alkoxy carbonylated using C1-4 alkyl chloroformate, and the resultant product is re-extracted using an organic solvent to obtain a re-extract; and the re-extract is acyl-derivated, and the concentration of estrogen is measured in the acyl-derivated product using a gas chromatography-mass spectrometry. The organic solvent is one or the mixture of normal hexane, normal pentane, butyl methyl ether, and ethyl acetate.
Abstract translation: 目的：提供从绝经后妇女获得的尿液和血液中雌激素代谢物的高灵敏度分析方法，以验证雌激素代谢的总体分布，并用于克服与雌激素代谢相关的疾病。 构成：从绝经后妇女获得的尿液和血液中雌激素代谢物的高灵敏度分析方法包括以下步骤：从绝经后妇女获得的尿液和血液中同时提取雌激素，并进行水解; 水解产物是使用氯甲酸C 1-4烷基羰基化烷氧基，所得产物用有机溶剂再萃取，得到再提取物; 并且再提取物是酰基衍生的，并且使用气相色谱 - 质谱法在酰基衍生的产物中测量雌激素的浓度。 有机溶剂是一种或正己烷，正戊烷，丁基甲基醚和乙酸乙酯的混合物。
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公开(公告)号：KR100785913B1
公开(公告)日：2007-12-17
申请号：KR1020060119300
申请日：2006-11-29
Applicant: 한국과학기술연구원
CPC classification number: C08F251/00 , C08B37/0012 , C08L5/16 , C08L51/02 , G01N33/548 , G01N33/743 , G01N2600/00 , C08L2666/02
Abstract: A cured beta-cyclodextrin polymer powder, its preparation method, and a filler for a solid phase extraction containing the cured beta-cyclodextrin polymer powder are provided to extract a steroid hormone present in a living body sample simply and effectively by using the cured beta-cyclodextrin polymer powder. A cured beta-cyclodextrin polymer powder comprises a beta-cyclodextrin polymer prepared by the reaction of a beta-cyclodextrin monomer and epichlorohydrin; and a metal ion which is coated on the surface of the beta-cyclodextrin polymer. Preferably the metal ion is selected from Ca and Na. The cured beta-cyclodextrin polymer powder is prepared by dissolving beta-cyclodextrin in an alkaline aqueous solution and adding epichlorohydrin to it to prepare a beta-cyclodextrin polymer of gel state; dipping the polymer in a metal salt aqueous solution to coat the polymer with a metal ion, thereby preparing a cured beta-cyclodextrin polymer; and washing, drying and pulverizing the obtained polymer.
Abstract translation: 提供固化的β-环糊精聚合物粉末，其制备方法和用于固相β-环糊精聚合物粉末的固相提取填料，通过使用固化的β-环糊精聚合物粉末提取活体样品中存在的类固醇激素， 环糊精聚合物粉末。 固化的β-环糊精聚合物粉末包含通过β-环糊精单体和表氯醇反应制备的β-环糊精聚合物; 以及涂覆在β-环糊精聚合物表面上的金属离子。 优选地，金属离子选自Ca和Na。 固化的β-环糊精聚合物粉末通过将β-环糊精溶解在碱性水溶液中并加入表氯醇来制备凝胶状态的β-环糊精聚合物来制备; 将聚合物浸渍在金属盐水溶液中以用金属离子涂覆聚合物，从而制备固化的β-环糊精聚合物; 并洗涤，干燥和粉碎所得聚合物。
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公开(公告)号：KR1020100095729A
公开(公告)日：2010-09-01
申请号：KR1020090014690
申请日：2009-02-23
Applicant: 한국과학기술연구원
IPC: G01N33/74 , G01N33/573 , G01N30/74
Abstract: PURPOSE: A method for analyzing difference of steroid hormone bio metabolite from two sample groups is provided to general metabolism tendency. CONSTITUTION: A method for analyzing difference of steroid hormone bio metabolite from two sample groups comprises: a step of extracting steroid hormone bio metabolite including male hormone, female hormone, corpus luteum hormone, sterol, and adrenal cortical hormone; a step of extracting the extracts again using an organic solvent; a step of measuring concentration of steroid hormone bio metabolite in urine by gas chromatography-mass spectrometry; and a step of comparing the concentration of liver steroid hormone bio metabolite between two samples. The organic solvent is a mixture solvent containing ethyl acetate and normal hexane.
Abstract translation: 目的：分析两种样品组类固醇激素生物代谢产物差异的一般方法。 构成：从两个样本组分析类固醇激素生物代谢物差异的方法包括：提取类固醇激素生物代谢物，包括雄性激素，雌激素，黄体激素，固醇和肾上腺皮质激素的步骤; 使用有机溶剂再次提取提取物的步骤; 通过气相色谱 - 质谱法测定尿液中类固醇激素生物代谢物的浓度的步骤; 并比较两个样品之间肝脏类固醇激素生物代谢物的浓度。 有机溶剂是含乙酸乙酯和正己烷的混合溶剂。
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公开(公告)号：KR100817934B1
公开(公告)日：2008-03-31
申请号：KR1020060119299
申请日：2006-11-29
Applicant: 한국과학기술연구원
IPC: C07J1/00
CPC classification number: C07J75/00
Abstract: A selective extraction method of a steroid compound using polymerized beta-cyclodextrin is provided to extract various shapes of the steroid hormones from a biological sample effectively through a simple process. A selective extraction method of a steroid compound using polymerized beta-cyclodextrin powder comprises the steps of: adding buffer solution and hydrolase to a liquid sample including the steroid compound; removing a water layer by centrifugation after adding and stirring hardened beta-cyclodextrin powder that beta-cyclodextrin monomer forms a layer structure by being bridged-bonded by epichlorohydrin and metal ions are spread between the layers of the bridged beta-cyclodextrin polymer to the reaction solution; and extracting the steroid compound by applying organic solvents after adding phosphate buffer solution and polar organic solvents, and controlling pH at a range of 9.0 - 10.0.
Abstract translation: 提供使用聚合β-环糊精的类固醇化合物的选择性提取方法，通过简单的方法有效地从生物样品中提取各种形状的类固醇激素。 使用聚合的β-环糊精粉末的类固醇化合物的选择性提取方法包括以下步骤：向包含类固醇化合物的液体样品中加入缓冲溶液和水解酶; 通过在加入和搅拌硬化的β-环糊精粉末之后通过离心除去水层，β-环糊精单体通过用表氯醇和金属离子桥接键合形成层结构，在桥连的β-环糊精聚合物层之间铺展到反应溶液 ; 并在加入磷酸盐缓冲液和极性有机溶剂后通过施加有机溶剂萃取类固醇化合物，并控制pH在9.0 - 10.0的范围内。
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