公开(公告)号：KR100863753B1

公开(公告)日：2008-10-16

申请号：KR1020070056043

申请日：2007-06-08

Applicant: 함평군

Inventor： 박성수 ,  김소현 ,  남성원 ,  이강무 ,  황혜진

IPC: C12M1/36 ,  C12M1/34 ,  C12M1/00 ,  G01N33/48

CPC classification number: C12M23/12 ,  C12M41/36 ,  C12M41/48 ,  G01N33/5005

Abstract: A micropost array is provided to measure C. elegans behaviors including chemotaxis, aerotaxis, thermotaxis and mechanosensation, detect abnormal C. elegans, perform toxicity test by using C. elegans, and screen a drug by supplying the similar environments to the wet soil where C. elegans inhabits. A micropost array(100) for quantitative measurement of C. elegans behaviors contains a plurality of microposts(120) having a circle section, diameter of 50-300 mm and height of 70-200 mm, arranged at regular distances of about 50-300 mm on a substrate(110), and has a water layer on the surface. A method for preparing the micropost array comprises the steps of: preparing a SU-8 master mold by using photolithography; preparing polydimethyl siloxane(PDMS) replica from the SU-8 master mold; and pouring 2-6%(w/v) agar into the PDMS replica as a template, and solidifying and separating the solid agar.

Abstract translation: 提供微孔阵列来测定秀丽隐杆线虫行为，包括趋化性，飞行时间，热感和机械补偿，检测异常秀丽隐杆线虫，通过使用秀丽隐杆线虫进行毒性试验，并通过向湿土提供相似的环境筛选药物，其中C 线虫居住。 用于线虫行为的定量测量的微孔阵列（100）包含多个微孔（120），其具有圆形截面，直径为50-300mm，高度为70-200mm，以约50-300的规则距离 mm，并且在表面上具有水层。 制备微孔阵列的方法包括以下步骤：通过使用光刻法制备SU-8母模; 从SU-8母模制备聚二甲基硅氧烷（PDMS）复制品; 并将2-6％（w / v）琼脂倒入PDMS复制品中作为模板，并固化和分离固体琼脂。