公开(公告)号：JPH11318452A

公开(公告)日：1999-11-24

申请号：JP3149799

申请日：1999-02-09

Applicant: BASF AG

Inventor： KROEGER BURKHARD DR ,  OTTERBACH BERND DR

IPC: C12N15/09 ,  A61K48/00 ,  C07K14/705 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/00 ,  C12N15/12 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/02 ,  C12Q1/68 ,  C12R1/91 ,  G01N33/15 ,  G01N33/53 ,  G01N33/566

Abstract: PROBLEM TO BE SOLVED: To obtain an isolated protein having an activity as a G protein-coupled receptor and useful for the treatment of diseases, etc., in the central nervous system or the peripheral nervous system such as Alzheimer disease by providing a specific amino acid sequence. SOLUTION: This receptor is a human protein having an amino acid sequence represented by the formula or a sequence in which one or more amino acid residues are substituted, inserted or deleted therefrom and retaining one or more main biological characteristics of the protein represented by the formula at that time. A cell having the protein on the cell surface is preferably combined with many substances to be investigated and the biological activity of the receptor is then compared in the presence (absence) of the substances to be tested to identify an antagonist and an agonist of the protein. A biological sample together with an antibody specifically to the protein is kept at a constant temperature to detect an antibody/antigen complex. The amount of the antibody/ antigen complex is preferably compared with the standard to qualitatively and quantitatively detect the protein in the biological sample.

公开(公告)号：DE19805351A1

公开(公告)日：1999-08-12

申请号：DE19805351

申请日：1998-02-11

Applicant: BASF AG

Inventor： KROEGER BURKHARD DR ,  OTTERBACH BERND DR

IPC: C12N15/09 ,  A61K48/00 ,  C07K14/705 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/00 ,  C12N15/12 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/02 ,  C12Q1/68 ,  C12R1/91 ,  G01N33/15 ,  G01N33/53 ,  G01N33/566 ,  C07K16/00 ,  C07H21/04 ,  C07K14/435 ,  C12N1/00 ,  C12N15/11 ,  C12Q1/00 ,  G01N33/68

Abstract: An isolated protein or its derivative (I) is new and has a 481 amino acid sequence given in the specification Independent claims are also included for the following: (1) a nucleic acid sequence (II) encoding (I); (2) recombinant constructs (IIa) comprising (II) plus regulatory elements; (3) host cells transformed with (II) or (IIa); (4) antibodies (Ab) specific for (I); (5) a method for identifying ligands of (I); and (6) methods for quantitative or qualitative determination of (I) and (II) in biological samples.