公开(公告)号：WO0131047A3

公开(公告)日：2002-02-07

申请号：PCT/EP0010552

申请日：2000-10-26

Applicant: BASF AG ,  SCHMID ANDREAS ,  WITHOLT BERNARD ,  HAUER BERNHARD ,  BUEHLER BRUNO

Inventor： SCHMID ANDREAS ,  WITHOLT BERNARD ,  HAUER BERNHARD ,  BUEHLER BRUNO

IPC: C12N15/09 ,  C12N1/21 ,  C12P7/24 ,  C12P7/40 ,  C12R1/19 ,  C12R1/38

CPC classification number: C12P7/40 ,  C12P7/24 ,  C12Q1/26

Abstract: The invention relates to a method for the step-wise oxidation of aromatic compounds to the corresponding aldehyde and/or carboxylic acid derivatives using genetically engineered microorganisms that express xylol monooxygenase or alkane monooxygenase.

Abstract translation: 本发明涉及一种用于使用二甲苯单加氧酶或芳族化合物的烷烃为相应的醛和/或羧酸衍生物的分步氧化的方法单加氧酶的表达，基因重组微生物。

公开(公告)号：NZ522223A

公开(公告)日：2006-02-24

申请号：NZ52222301

申请日：2001-05-16

Applicant: BASF AG

Inventor： SCHMID ANDREAS ,  HOLLMANN FRANK ,  HAUER BERNHARD ,  ZELINSKI THOMAS ,  STECKHAN EBERHARD

IPC: C12P1/00 ,  C12P7/22 ,  C25B3/25 ,  C12M1/40 ,  C25B3/04 ,  C25B9/00 ,  C12M1/00

Abstract: A method comprising the indirect electrochemical regeneration of NAD(P)H in enzymatic substrate conversions which are catalyzed by monooxygenases is described. In particular, a method for the electroenzymatic preparation of 2,3-dihydroxyphenyl derivatives, which is catalyzed by the enzyme 2-hydroxybiphenyl 3-monooxygenase and in which simultaneously the NAD+ formed by reductive cleavage of oxygen is electrochemically reduced, is described.

公开(公告)号：NO20021906L

公开(公告)日：2002-04-26

申请号：NO20021906

申请日：2002-04-23

Applicant: BASF AG

Inventor： SCHMID ANDREAS ,  WITHOLD BERNARD ,  HAUER BERNHARD ,  BUEHLER BRUNO

IPC: C12N15/09 ,  C12N1/21 ,  C12P7/24 ,  C12P7/40 ,  C12R1/19 ,  C12R1/38

Abstract: Production of aromatic aldehyde or carboxylic acid (I) by culturing a microorganism that expresses xylene mono-oxygenase (XMO) or alkane mono-oxygenase (AMO) in a medium containing an aromatic substrate (II). Production of aromatic aldehyde or carboxylic acid of formula (I) by culturing a microorganism that expresses xylene mono-oxygenase (XMO) or alkane mono-oxygenase (AMO) in a medium containing an aromatic substrate of formula (II). [Image] Ar : monocyclic aromatic ring, optionally with one or more substituents; R 1>formyl or carboxy; n : 0-15; R 2>vinyl or (CH 2) m-R 3>, also where R 1>= carboxy, R 2>may be (CH 2) n-CHO; m : n+1; and R 3>hydrogen or hydroxy. Independent claims are also included for the following: (a) recombinant microorganism harboring an expression vector that contains, under control of the alk-regulatory system of Pseudomonas oleovoransGpol, the XMO-encoding genes xylM and xylA, or the AMO-encoding genes alkB, alkG and alkT; and (b) expression construct containing the genes specified in (a), operably linked to the alk-regulatory system.

公开(公告)号：CA2389138A1

公开(公告)日：2001-05-03

申请号：CA2389138

申请日：2000-10-26

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  WITHOLT BERNARD ,  SCHMID ANDREAS ,  BUHLER BRUNO

IPC: C12N15/09 ,  C12N1/21 ,  C12P7/24 ,  C12P7/40 ,  C12R1/19 ,  C12R1/38

Abstract: The invention relates to a method for the step-wise oxidation of aromatic compounds to the corresponding aldehyde and/or carboxylic acid derivatives using genetically engineered microorganisms that express xylol monooxygenase or alkane monooxygenase.

公开(公告)号：CA2442561C

公开(公告)日：2011-09-27

申请号：CA2442561

申请日：2002-04-05

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  ZELINSKI THOMAS ,  WITHOLT BERNHARD ,  SCHMID ANDREAS ,  BUEHLER BRUNO

IPC: C12N15/09 ,  C12P7/24 ,  C12P7/22 ,  C12P7/40 ,  C12R1/40

Abstract: The invention relates to a method for the biocatalytic oxidation of aromatic compounds, using recombinant micro-organisms expressing xylene monooxygenase, in a two-phase reaction medium.

公开(公告)号：NO20065545L

公开(公告)日：2006-12-12

申请号：NO20065545

申请日：2006-12-01

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  SCHMID ANDREAS ,  HOLLMAN FRANK ,  HOFSTETTER KARIN ,  HABICHER TILO

IPC: C12P7/00 ,  C12N9/02 ,  C12P1/00

Abstract: Process for an enzymatic oxygenation catalyzed by a FAD-dependant monooxygenase and direct electrochemical regeneration of the FAD-dependant monooxygenase

公开(公告)号：AT349543T

公开(公告)日：2007-01-15

申请号：AT01929648

申请日：2001-05-16

Applicant: BASF AG

Inventor： STECKHAN EBERHARD ,  SCHMID ANDREAS ,  HOLLMANN FRANK ,  HAUER BERNHARD ,  ZELINSKI THOMAS

IPC: C12M1/40 ,  C12P1/00 ,  C12P7/22 ,  C25B3/25 ,  C25B3/04 ,  C25B9/00 ,  C12M1/00

Abstract: A method comprising the indirect electrochemical regeneration of NAD(P)H in enzymatic substrate conversions which are catalyzed by monooxygenases, for example, is described. In particular, a method for the electroenzymatic preparation of 2,3-dihydroxyphenyl derivatives, which is catalyzed by the enzyme 2-hydroxybiphenyl 3-monooxygenase and in which simultaneously the NAD + formed by reductive cleavage of oxygen is electrochemically reduced, is described.

公开(公告)号：AU2001256357B2

公开(公告)日：2006-09-28

申请号：AU2001256357

申请日：2001-05-16

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  SCHMID ANDREAS ,  ZELINSKI THOMAS ,  HOLLMANN FRANK

IPC: C12M1/40 ,  C12P1/00 ,  C12P7/22 ,  C25B3/25

Abstract: A method comprising the indirect electrochemical regeneration of NAD(P)H in enzymatic substrate conversions which are catalyzed by monooxygenases, for example, is described. In particular, a method for the electroenzymatic preparation of 2,3-dihydroxyphenyl derivatives, which is catalyzed by the enzyme 2-hydroxybiphenyl 3-monooxygenase and in which simultaneously the NAD + formed by reductive cleavage of oxygen is electrochemically reduced, is described.

公开(公告)号：DK1224315T3

公开(公告)日：2006-07-10

申请号：DK00975925

申请日：2000-10-26

Applicant: BASF AG

Inventor： SCHMID ANDREAS ,  BUEHLER BRUNO ,  WITHOLT BERNARD ,  HAUER BERNHARD

IPC: C12N15/09 ,  C12P7/24 ,  C12N1/21 ,  C12P7/40 ,  C12R1/19 ,  C12R1/38

Abstract: Production of aromatic aldehyde or carboxylic acid (I) by culturing a microorganism that expresses xylene mono-oxygenase (XMO) or alkane mono-oxygenase (AMO) in a medium containing an aromatic substrate (II). Production of aromatic aldehyde or carboxylic acid of formula (I) by culturing a microorganism that expresses xylene mono-oxygenase (XMO) or alkane mono-oxygenase (AMO) in a medium containing an aromatic substrate of formula (II). [Image] Ar : monocyclic aromatic ring, optionally with one or more substituents; R 1>formyl or carboxy; n : 0-15; R 2>vinyl or (CH 2) m-R 3>, also where R 1>= carboxy, R 2>may be (CH 2) n-CHO; m : n+1; and R 3>hydrogen or hydroxy. Independent claims are also included for the following: (a) recombinant microorganism harboring an expression vector that contains, under control of the alk-regulatory system of Pseudomonas oleovoransGpol, the XMO-encoding genes xylM and xylA, or the AMO-encoding genes alkB, alkG and alkT; and (b) expression construct containing the genes specified in (a), operably linked to the alk-regulatory system.

公开(公告)号：DE50012376D1

公开(公告)日：2006-05-04

申请号：DE50012376

申请日：2000-10-26

Applicant: BASF AG

Inventor： SCHMID ANDREAS ,  WITHOLT BERNARD ,  HAUER BERNHARD ,  BUEHLER BRUNO

IPC: C12N15/09 ,  C12P7/24 ,  C12N1/21 ,  C12P7/40 ,  C12R1/19 ,  C12R1/38

Abstract: Production of aromatic aldehyde or carboxylic acid (I) by culturing a microorganism that expresses xylene mono-oxygenase (XMO) or alkane mono-oxygenase (AMO) in a medium containing an aromatic substrate (II). Production of aromatic aldehyde or carboxylic acid of formula (I) by culturing a microorganism that expresses xylene mono-oxygenase (XMO) or alkane mono-oxygenase (AMO) in a medium containing an aromatic substrate of formula (II). [Image] Ar : monocyclic aromatic ring, optionally with one or more substituents; R 1>formyl or carboxy; n : 0-15; R 2>vinyl or (CH 2) m-R 3>, also where R 1>= carboxy, R 2>may be (CH 2) n-CHO; m : n+1; and R 3>hydrogen or hydroxy. Independent claims are also included for the following: (a) recombinant microorganism harboring an expression vector that contains, under control of the alk-regulatory system of Pseudomonas oleovoransGpol, the XMO-encoding genes xylM and xylA, or the AMO-encoding genes alkB, alkG and alkT; and (b) expression construct containing the genes specified in (a), operably linked to the alk-regulatory system.