公开(公告)号：NO20021906L

公开(公告)日：2002-04-26

申请号：NO20021906

申请日：2002-04-23

Applicant: BASF AG

Inventor： SCHMID ANDREAS ,  WITHOLD BERNARD ,  HAUER BERNHARD ,  BUEHLER BRUNO

IPC: C12N15/09 ,  C12N1/21 ,  C12P7/24 ,  C12P7/40 ,  C12R1/19 ,  C12R1/38

Abstract: Production of aromatic aldehyde or carboxylic acid (I) by culturing a microorganism that expresses xylene mono-oxygenase (XMO) or alkane mono-oxygenase (AMO) in a medium containing an aromatic substrate (II). Production of aromatic aldehyde or carboxylic acid of formula (I) by culturing a microorganism that expresses xylene mono-oxygenase (XMO) or alkane mono-oxygenase (AMO) in a medium containing an aromatic substrate of formula (II). [Image] Ar : monocyclic aromatic ring, optionally with one or more substituents; R 1>formyl or carboxy; n : 0-15; R 2>vinyl or (CH 2) m-R 3>, also where R 1>= carboxy, R 2>may be (CH 2) n-CHO; m : n+1; and R 3>hydrogen or hydroxy. Independent claims are also included for the following: (a) recombinant microorganism harboring an expression vector that contains, under control of the alk-regulatory system of Pseudomonas oleovoransGpol, the XMO-encoding genes xylM and xylA, or the AMO-encoding genes alkB, alkG and alkT; and (b) expression construct containing the genes specified in (a), operably linked to the alk-regulatory system.

公开(公告)号：NO20021906A

公开(公告)日：2002-04-26

申请号：NO20021906

申请日：2002-04-23

Applicant: BASF AG

Inventor： SCHMID ANDREAS ,  WITHOLD BERNARD ,  HAUER BERNHARD ,  BUEHLER BRUNO

IPC: C12N15/09 ,  C12N1/21 ,  C12P7/24 ,  C12P7/40 ,  C12R1/19 ,  C12R1/38

CPC classification number: C12P7/40 ,  C12P7/24 ,  C12Q1/26

公开(公告)号：NO20021906D0

公开(公告)日：2002-04-23

申请号：NO20021906

申请日：2002-04-23

Applicant: BASF AG

Inventor： SCHMID ANDREAS ,  WITHOLD BERNARD ,  HAUER BERNHARD ,  BUEHLER BRUNO

IPC: C12N15/09 ,  C12N1/21 ,  C12P7/24 ,  C12P7/40 ,  C12R1/19 ,  C12R1/38 ,  C12P

Abstract: Production of aromatic aldehyde or carboxylic acid (I) by culturing a microorganism that expresses xylene mono-oxygenase (XMO) or alkane mono-oxygenase (AMO) in a medium containing an aromatic substrate (II). Production of aromatic aldehyde or carboxylic acid of formula (I) by culturing a microorganism that expresses xylene mono-oxygenase (XMO) or alkane mono-oxygenase (AMO) in a medium containing an aromatic substrate of formula (II). [Image] Ar : monocyclic aromatic ring, optionally with one or more substituents; R 1>formyl or carboxy; n : 0-15; R 2>vinyl or (CH 2) m-R 3>, also where R 1>= carboxy, R 2>may be (CH 2) n-CHO; m : n+1; and R 3>hydrogen or hydroxy. Independent claims are also included for the following: (a) recombinant microorganism harboring an expression vector that contains, under control of the alk-regulatory system of Pseudomonas oleovoransGpol, the XMO-encoding genes xylM and xylA, or the AMO-encoding genes alkB, alkG and alkT; and (b) expression construct containing the genes specified in (a), operably linked to the alk-regulatory system.