AN ELECTROPHORESIS SYSTEM AND A SEPARATION AND IDENTIFICATION METHOD

    公开(公告)号:HK1206430A1

    公开(公告)日:2016-01-08

    申请号:HK15107072

    申请日:2015-07-24

    Abstract: Electrophoresis system comprising at least one type of electrophoresis gel card, at least one type of blot membrane card, an electrophoresis apparatus for running electrophoresis experiments using the electrophoretic gel card, a blot transfer unit for transfer of separated sample from the electrophoresis gel card to the blot membrane card, an imaging apparatus for recording images of separated sample in the electrophoresis gel card and the blot membrane card, wherein; the electrophoresis gel card and the blot membrane card each comprises a rigid support provided with an alignment structure defining a positional reference for mutual alignment during transfer, and for alignment with respect to a complementary alignment structure in the imaging apparatus to provide mechanically aligned images of separated sample in the electrophoresis gel card and the blot membrane card.

    AN ELECTROPHORESIS SYSTEM AND A SEPARATION AND IDENTIFICATION METHOD

    公开(公告)号:IN9271DEN2014A

    公开(公告)日:2015-07-10

    申请号:IN9271DEN2014

    申请日:2014-11-05

    Abstract: Electrophoresis system comprising at least one type of electrophoresis gel card at least one type of blot membrane card an electrophoresis apparatus for running electrophoresis experiments using the electrophoretic gel card a blot transfer unit for transfer of separated sample from the electrophoresis gel card to the blot membrane card an imaging apparatus for recording images of separated sample in the electrophoresis gel card and the blot membrane card wherein; the electrophoresis gel card and the blot membrane card each comprises a rigid support provided with an alignment structure defining a positional reference for mutual alignment during transfer and for alignment with respect to a complementary alignment structure in the imaging apparatus to provide mechanically aligned images of separated sample in the electrophoresis gel card and the blot membrane card.

    METHOD FOR SPECIFIC IDENTIFICATION OF TARGET BIOMOLECULES
    10.
    发明公开
    METHOD FOR SPECIFIC IDENTIFICATION OF TARGET BIOMOLECULES 审中-公开
    方法靶生物分子的特异性检测

    公开(公告)号:EP2726877A4

    公开(公告)日:2015-04-08

    申请号:EP12803774

    申请日:2012-06-27

    CPC classification number: G01N33/543 G01N27/44726 G01N33/561

    Abstract: The present invention relates to a method for identification of specific target proteins in a protein sample following a detection procedure, such as a Western blotting procedure, wherein the membrane is probed with at least two primary antibodies directed against the same and/or different epitopes of the same target protein, and wherein specific binding to the target protein in a sample is differentiated from unspecific binding to the target protein by comparing the resulting sample patterns, such as bands or spot patterns, with each other. In a further step signals from the true target proteins are enhanced while signals resulting from unspecific binding are diminished.

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