Abstract:
Electrophoresis system comprising at least one type of electrophoresis gel card, at least one type of blot membrane card, an electrophoresis apparatus for running electrophoresis experiments using the electrophoretic gel card, a blot transfer unit for transfer of separated sample from the electrophoresis gel card to the blot membrane card, an imaging apparatus for recording images of separated sample in the electrophoresis gel card and the blot membrane card, wherein; the electrophoresis gel card and the blot membrane card each comprises a rigid support provided with an alignment structure defining a positional reference for mutual alignment during transfer, and for alignment with respect to a complementary alignment structure in the imaging apparatus to provide mechanically aligned images of separated sample in the electrophoresis gel card and the blot membrane card.
Abstract:
Electrophoresis system comprising at least one type of electrophoresis gel card at least one type of blot membrane card an electrophoresis apparatus for running electrophoresis experiments using the electrophoretic gel card a blot transfer unit for transfer of separated sample from the electrophoresis gel card to the blot membrane card an imaging apparatus for recording images of separated sample in the electrophoresis gel card and the blot membrane card wherein; the electrophoresis gel card and the blot membrane card each comprises a rigid support provided with an alignment structure defining a positional reference for mutual alignment during transfer and for alignment with respect to a complementary alignment structure in the imaging apparatus to provide mechanically aligned images of separated sample in the electrophoresis gel card and the blot membrane card.
Abstract:
Electrophoresis gel cassette comprising a first and a second face wall member and one or more side wall members defining a gel compartment for a gel member with a first and second face wherein the first face wall member has high gel adhesion compared to the second face wall member whereby a gel member molded in the cassette will stay attached to the high gel adhesion face wall member when the cassette is opened and wherein the first face wall member is provided with at least one removable section to expose a section of the first face of the gel member the removable section of the first face wall member having lower gel adhesion compared to the non removable part of the first face wall member.
Abstract:
Electrophoresis gel unit comprising a flat gel member with an upper and a lower face and a sample separation zone wherein the gel member is attached to a rigid support arranged to preserve the shape of and to facilitate handling of the gel member wherein the rigid support is formed to allow access to a section of both the upper and lower face of the gel member essentially corresponding to the separation zone.
Abstract:
Electrophoresis gel unit comprising a flat gel member with an upper and a lower face and a sample separation zone, wherein the gel member is attached to a rigid support arranged to preserve the shape of and to facilitate handling of the gel member, wherein the rigid support is formed to allow access to a section of both the upper and lower face of the gel member essentially corresponding to the separation zone.
Abstract:
The present invention relates to a method for identification of specific target proteins in a protein sample following a detection procedure, such as a Western blotting procedure, wherein the membrane is probed with at least two primary antibodies directed against the same and/or different epitopes of the same target protein, and wherein specific binding to the target protein in a sample is differentiated from unspecific binding to the target protein by comparing the resulting sample patterns, such as bands or spot patterns, with each other. In a further step signals from the true target proteins are enhanced while signals resulting from unspecific binding are diminished.