公开(公告)号：EP0123372A2

公开(公告)日：1984-10-31

申请号：EP84300516.6

申请日：1984-01-27

Applicant: STAUFFER CHEMICAL COMPANY

Inventor： Okubara, Patricia Ann ,  Pierce, Dorothy Anne ,  Nutter, Robert Clair ,  Howard, John Anthony

IPC: C12N15/00

CPC classification number: C12N9/0028 ,  C12N15/81 ,  C12N15/8205

Abstract: Decombinant plasmids adopted for transformation of prokaryotic and eukaryotic hosts having an insertion site for at least one DNA fragment are provided. In a preferred embodiment, nucleotide sequences coding for methotrexate-resistant dehydrofolate reductase and a nucleotide sequence, a portion thereof coding for a promotor, are ligated into the insertion site.

Abstract translation: 提供了用于转化具有至少一个DNA片段的插入位点的原核和真核宿主的重组质粒。 在优选的实施方案中，编码氨甲蝶呤抗性脱氢叶酸还原酶的核苷酸序列和编码启动子的部分核苷酸序列连接到插入位点。

公开(公告)号：EP0123372A3

公开(公告)日：1986-02-12

申请号：EP84300516

申请日：1984-01-27

Applicant: STAUFFER CHEMICAL COMPANY

Inventor： Okubara, Patricia Ann ,  Pierce, Dorothy Anne ,  Nutter, Robert Clair ,  Howard, John Anthony

IPC: C12N15/00

CPC classification number: C12N9/0028 ,  C12N15/81 ,  C12N15/8205

Abstract: Decombinant plasmids adopted for transformation of prokaryotic and eukaryotic hosts having an insertion site for at least one DNA fragment are provided. In a preferred embodiment, nucleotide sequences coding for methotrexate-resistant dehydrofolate reductase and a nucleotide sequence, a portion thereof coding for a promotor, are ligated into the insertion site.

Abstract translation: 提供了用于转化具有至少一个DNA片段的插入位点的原核和真核宿主的重组质粒。 在优选的实施方案中，编码氨甲蝶呤抗性脱氢叶酸还原酶的核苷酸序列和编码启动子的部分核苷酸序列连接到插入位点。