公开(公告)号：WO1996017055A1

公开(公告)日：1996-06-06

申请号：PCT/US1995015714

申请日：1995-12-01

Applicant: VANDERBILT UNIVERSITY

Inventor： VANDERBILT UNIVERSITY ,  HAWIGER, Jack, J. ,  DONAHUE, John, P. ,  PATEL, Hareshkumar ,  ANDERSON, Wayne, F.

IPC: C12N09/36

CPC classification number: C12N15/62 ,  C07K14/47 ,  C07K14/75 ,  C07K16/065 ,  C07K2319/00 ,  C12N9/2462 ,  C30B7/00 ,  C30B29/58

Abstract: The invention relates to methods of crystallizing a peptide or polypeptide comprising linking a peptide or polypeptide to a terminus of a crystallizable carrier protein to form a chimeric protein and crystallizing the chimeric protein. The invention also includes methods for determining the three-dimensional structure of a peptide or polypeptide, the crystallized chimeric proteins themselves, and methods for designing a peptide or polypeptide for screening for improved binding to a molecule by using the three dimensional structural information.

Abstract translation: 本发明涉及使肽或多肽结晶的方法，包括将肽或多肽与可结晶载体蛋白的末端连接以形成嵌合蛋白并使嵌合蛋白结晶。 本发明还包括用于确定肽或多肽的三维结构，结晶的嵌合蛋白本身的方法，以及用于设计肽或多肽以筛选通过使用三维结构信息改善与分子的结合的方法。