公开(公告)号：CN107429275A

公开(公告)日：2017-12-01

申请号：CN201680017395.3

申请日：2016-01-27

Applicant: 丹麦技术大学

Inventor： 赫曼舒·蒙德哈达 ,  阿列克斯·措夫特高·尼尔森

IPC: C12P13/00 ,  C12P13/04 ,  C12P13/06 ,  C12P13/12 ,  C12P13/22 ,  C12P17/16 ,  C12P17/18

CPC classification number: C12N1/36 ,  C07K14/245 ,  C07K14/32 ,  C12N9/0006 ,  C12N9/1014 ,  C12N9/1096 ,  C12N9/1205 ,  C12N9/1217 ,  C12N9/1247 ,  C12N9/16 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12N15/01 ,  C12N15/52 ,  C12P13/001 ,  C12P13/06 ,  C12P13/12 ,  C12P13/22 ,  C12P17/165 ,  C12P17/167 ,  C12Y101/01003 ,  C12Y101/01095 ,  C12Y201/02001 ,  C12Y206/01052 ,  C12Y207/02003 ,  C12Y301/03003 ,  C12Y403/01017 ,  C12P13/04 ,  C12P17/18

Abstract: 本发明总体涉及微生物工业，并且具体涉及使用基因修饰的细菌的L-丝氨酸或L-丝氨酸衍生物的生产。本发明提供了基因修饰的微生物，如细菌，其中通过如灭活来减弱编码参与L-丝氨酸降解的酶的基因的表达，这使得它们特别适用于以较高的产率生产L-丝氨酸。本发明还提供了藉此使微生物，且更具体地细菌可以对更高浓度丝氨酸耐受性的方法。本发明还提供了用于使用这种基因修饰的微生物生产L-丝氨酸或L-丝氨酸衍生物的方法。

公开(公告)号：CN105861534A

公开(公告)日：2016-08-17

申请号：CN201610325584.9

申请日：2016-05-12

Applicant: 中国药科大学

Inventor： 许激扬 ,  卞筱泓 ,  孙天娇

IPC: C12N15/72 ,  C12N15/66 ,  C12P17/18

CPC classification number: C12N15/72 ,  C12N9/0028 ,  C12N9/1014 ,  C12N15/66 ,  C12N2800/101 ,  C12N2800/60 ,  C12P17/188 ,  C12Y105/0102 ,  C12Y201/02001

Abstract: 本发明提供了一种提高L?5?甲基四氢叶酸累积量的生物合成方法和和一种L?5?甲基四氢叶酸合成酶系共表达重组质粒及其构建方法和应用。本发明提供的L?5?甲基四氢叶酸合成酶系共表达重组质粒包括丝氨酸羟甲基转移酶(SHMT)基因GlyA和亚甲基四氢叶酸还原酶(MTHFR)基因MetF序列。本发明提供的提高L?5?甲基四氢叶酸累积量的生物合成方法为，将L?5?甲基四氢叶酸合成酶系共表达重组质粒转化累积L?5?甲基四氢叶酸原始菌株，获得重组菌并发酵该重组菌。最终发酵产物中，重组菌累积的L?5?甲基四氢叶酸显著高于原始菌株，提高了原料利用率，降低了生产成本和能耗。

公开(公告)号：CN107406864A

公开(公告)日：2017-11-28

申请号：CN201680017391.5

申请日：2016-01-27

Applicant: 丹麦技术大学

Inventor： 赫曼舒·蒙德哈达 ,  阿列克斯·措夫特高·尼尔森

IPC: C12P13/00 ,  C12P13/04 ,  C12P13/06 ,  C12P13/12 ,  C12P13/22 ,  C12P17/16 ,  C12P17/18

CPC classification number: C12N1/36 ,  C07K14/245 ,  C07K14/32 ,  C12N9/0006 ,  C12N9/1014 ,  C12N9/1096 ,  C12N9/1205 ,  C12N9/1217 ,  C12N9/1247 ,  C12N9/16 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12N15/01 ,  C12N15/52 ,  C12P13/001 ,  C12P13/06 ,  C12P13/12 ,  C12P13/22 ,  C12P17/165 ,  C12P17/167 ,  C12Y101/01003 ,  C12Y101/01095 ,  C12Y201/02001 ,  C12Y206/01052 ,  C12Y207/02003 ,  C12Y301/03003 ,  C12Y403/01017 ,  C12P13/04 ,  C12P17/18

Abstract: 本发明总体涉及微生物工业，并且具体涉及使用基因修饰的细菌的L-丝氨酸生产。本发明提供了基因修饰的微生物，如细菌，其中如通过灭活来减弱编码参与L-丝氨酸降解的酶的基因的表达，这使得它们特别适用于以较高的产率生产L-丝氨酸。本发明还提供了藉此使微生物，且更具体地细菌可以对更高浓度丝氨酸耐受性的方法。本发明还提供了用于使用这种基因修饰的微生物生产L-丝氨酸或L-丝氨酸衍生物的方法。

公开(公告)号：US09676641B2

公开(公告)日：2017-06-13

申请号：US14605320

申请日：2015-01-26

Applicant: SynPhaGen LLC.

Inventor： Todd Bernard Parsley ,  Christian Furlan Freguia

IPC: A61K35/76 ,  C12N7/01 ,  C12N7/04 ,  C12N15/63 ,  C02F3/34 ,  C12N15/86 ,  C12N15/113 ,  C12N15/11

CPC classification number: C02F3/34 ,  A01N63/02 ,  A61K38/2066 ,  A61K38/45 ,  A61K38/482 ,  A61K48/005 ,  C12N7/00 ,  C12N15/111 ,  C12N15/113 ,  C12N15/70 ,  C12N15/86 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2320/32 ,  C12N2330/50 ,  C12N2795/00021 ,  C12N2795/00031 ,  C12N2795/00032 ,  C12N2795/00043 ,  C12N2795/14143 ,  C12N2820/55 ,  C12Y201/02001 ,  C12Y203/02013

Abstract: Compositions for a phage particle are disclosed. The phage particle is non-replicating and includes at least one heterologous nucleic acid sequence that is capable of being expressed in a target bacteria. The expressed heterologous nucleic acid sequence is non-lethal to the target bacteria.

公开(公告)号：US20160367701A1

公开(公告)日：2016-12-22

申请号：US15114634

申请日：2015-01-26

Applicant: SYNPHAGEN LLC.

Inventor： Todd Bernard Parsley ,  Christian Furlan Freguia

IPC: A61K48/00 ,  A01N63/02 ,  C12N15/70 ,  A61K38/20 ,  C12N15/113 ,  C12N7/00 ,  A61K38/45

CPC classification number: C02F3/34 ,  A01N63/02 ,  A61K38/2066 ,  A61K38/45 ,  A61K38/482 ,  A61K48/005 ,  C12N7/00 ,  C12N15/111 ,  C12N15/113 ,  C12N15/70 ,  C12N15/86 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2320/32 ,  C12N2330/50 ,  C12N2795/00021 ,  C12N2795/00031 ,  C12N2795/00032 ,  C12N2795/00043 ,  C12N2795/14143 ,  C12N2820/55 ,  C12Y201/02001 ,  C12Y203/02013

Abstract: Compositions for a phage particle are disclosed. The phage particle is non-replicating and includes at least one heterologous nucleic acid sequence that is capable of being expressed in a target bacteria. The expressed heterologous nucleic acid sequence is non-lethal to the target bacteria.

Abstract translation: 公开了噬菌体颗粒的组合物。 噬菌体颗粒是非复制型的，并且包括能够在靶细菌中表达的至少一种异源核酸序列。 表达的异源核酸序列对靶细菌是非致死的。

公开(公告)号：US20230285508A1

公开(公告)日：2023-09-14

申请号：US18198519

申请日：2023-05-17

Applicant: SynPhaGen LLC

Inventor： Todd Bernard Parsley ,  Christian Furlan Freguia

IPC: A61K38/20 ,  C02F3/34 ,  A61K38/45 ,  C12N15/113 ,  C12N15/86 ,  A61K38/48 ,  C12N15/11 ,  A61K48/00 ,  C12N15/70 ,  C12N7/00

CPC classification number: A61K38/2066 ,  C02F3/34 ,  A61K38/45 ,  C12N15/113 ,  C12N15/86 ,  C12Y203/02013 ,  A61K38/482 ,  C12N15/111 ,  A61K48/005 ,  C12N15/70 ,  C12N7/00 ,  C12Y201/02001 ,  C12N2310/14 ,  C12N2795/00031 ,  C12N2320/32 ,  C12N2330/50 ,  C12N2795/00032 ,  C12N2795/00021 ,  C12N2820/55 ,  C12N2795/14143 ,  C12N2795/00043 ,  C12N2310/531

Abstract: Compositions for a phage particle are disclosed. The phage particle is non-replicating and includes at least one heterologous nucleic acid sequence that is capable of being expressed in a target bacteria. The expressed heterologous nucleic acid sequence is non-lethal to the target bacteria.

公开(公告)号：US20160137701A1

公开(公告)日：2016-05-19

申请号：US14902484

申请日：2014-07-03

Applicant: OXTHERA INTELLECTUAL PROPERTY AB

Inventor： Elisabeth LINDNER ,  Helena COWLEY ,  Aaron COWLEY

IPC: C07K14/195 ,  C12N9/10 ,  C12N9/88 ,  C12N9/06 ,  C12N9/14 ,  C12N9/04 ,  C12N9/02

CPC classification number: C07K14/195 ,  A61K35/74 ,  A61K38/00 ,  A61K38/164 ,  C12N9/0006 ,  C12N9/0016 ,  C12N9/0051 ,  C12N9/1014 ,  C12N9/1085 ,  C12N9/14 ,  C12N9/88 ,  C12Y101/0106 ,  C12Y104/01016 ,  C12Y108/01 ,  C12Y201/02001 ,  C12Y205/01006 ,  C12Y205/01009 ,  C12Y205/01047 ,  C12Y303/01001 ,  C12Y402/01003

Abstract: The present invention relates to a secretagogue compound derived from oxalate degrading bacteria, for use in the treatment of an oxalate related disease and/or oxalate related imbalance in a subject, wherein the administration of the secretagogue results in a reduction of urinary oxalate and/or plasma oxalate in the subject. The invention further relates to a pharmaceutical composition comprising such a secretagogue compound, a method for treating a subject suffering from an oxalate related disease, and to a method for preparing a secretagogue.

Abstract translation: 本发明涉及一种衍生自草酸降解细菌的促分泌素化合物，用于治疗受试者中草酸盐相关疾病和/或草酸盐相关不平衡，其中所述促分泌素的施用导致尿草酸盐和/或 受试者血浆草酸盐。 本发明还涉及包含这种促分泌素化合物的药物组合物，用于治疗患有草酸盐相关疾病的受试者的方法和用于制备促分泌素的方法。

公开(公告)号：US07851187B2

公开(公告)日：2010-12-14

申请号：US11942873

申请日：2007-11-20

Applicant: Shinji Kuroda ,  Hiroyuki Nozaki ,  Kunihiko Watanabe ,  Kenzo Yokozeki ,  Yuki Imabayashi

Inventor： Shinji Kuroda ,  Hiroyuki Nozaki ,  Kunihiko Watanabe ,  Kenzo Yokozeki ,  Yuki Imabayashi

IPC: C12P13/06 ,  C12N9/00 ,  C12N9/10 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC classification number: C12N9/1014 ,  C12P13/06 ,  C12Y201/02001

Abstract: The present invention provides a new method for producing serine derivatives and their optically-activated derivatives in a convenient manner. In the presence of an enzyme, an L-α-amino acid of formula (I): (in the formula (I), R1 is a hydrocarbon group) is reacted with an aldehyde of formula (II): (in the formula (II), R2 is a hydrocarbon) to produce an L-serine derivative of formula (III). .

Abstract translation: 本发明提供了以方便的方式制备丝氨酸衍生物及其光活性衍生物的新方法。 在酶的存在下，式（I）:(式（I）中的R 1为烃基）的式（I）的L-α-氨基酸与式（II）的醛反应：（在式 II），R 2为烃），得到式（III）的L-丝氨酸衍生物。 。

公开(公告)号：US20240287443A1

公开(公告)日：2024-08-29

申请号：US18573085

申请日：2022-06-24

Applicant: Reliance Industries Limited

Inventor： Manmeet AHUJA ,  Chitranshu KUMAR ,  Anamika UPADHYAY ,  Vikas Kumar PATEL ,  Niraja SONI ,  Santanu DASGUPTA ,  Mansi Pankaj VORA

IPC: C12N1/12 ,  C07K14/435 ,  C12N9/10 ,  C12R1/89

CPC classification number: C12N1/125 ,  C07K14/43518 ,  C12N9/1014 ,  C12R2001/89 ,  C12Y201/02001

Abstract: The present disclosure relates to recombinant algae, more particularly to recombinant blue green algae. The disclosure further relates to a method for production of recombinant spider silk protein from the said recombinant algae. The said recombinant algae provides for green technology for the production of spider silk protein. The production of spider silk protein in the said recombinant algae is simple and economical.

公开(公告)号：US20240167066A1

公开(公告)日：2024-05-23

申请号：US18283610

申请日：2022-03-24

Applicant: DAICEL CORPORATION

Inventor： Akira OTANI ,  Hiroaki YAMAMOTO ,  Kazuya MITSUHASHI ,  Takafumi MITACHI ,  Satoru FUKIYA ,  Atsushi YOKOTA

IPC: C12P17/06 ,  C12N1/20 ,  C12N9/06 ,  C12N9/10 ,  C12P7/22 ,  C12P17/12 ,  C12R1/185 ,  C12R1/22 ,  C12R1/225 ,  C12R1/46

CPC classification number: C12P17/06 ,  C12N1/205 ,  C12N9/003 ,  C12N9/1014 ,  C12P7/22 ,  C12P17/12 ,  C12R2001/185 ,  C12R2001/22 ,  C12R2001/225 ,  C12R2001/46 ,  C12Y201/02001

Abstract: An object of the present disclosure is at least to provide a technique for promoting elimination of a methyl group(s) of a methoxy group(s) in causing a microorganism having a demethylation ability of eliminating a methyl group(s) of a methoxy group(s) from a compound with the methoxy group(s) in a side chain(s) to produce a demethylated compound in which a methyl group(s) of a methoxy group(s) has eliminated from a compound with the methoxy group(s) in a side chain(s). The issue is solved by a method for producing a demethylated compound, comprising co-culturing, in a solution containing a compound with a methoxy group(s) in a side chain(s), a microorganism having a demethylation ability of eliminating a methyl group(s) of a methoxy group(s) from a compound with the methoxy group(s) in a side chain(s), and a microorganism having an activity to promote the demethylation, to produce the demethylated compound in which a methyl group(s) of a methoxy group(s) has eliminated from the compound with the methoxy group(s) in the side chain(s).