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    ASSAY METHODS
    91.
    发明申请
    ASSAY METHODS 有权
    测定方法

    公开(公告)号：US20140357513A1

    公开(公告)日：2014-12-04

    申请号：US14331112

    申请日：2014-07-14

    Applicant: Fluidigm Corporation

    Inventor： Michael Lucero ,  Marc Unger

    IPC: C12Q1/68 ,  G01N33/53

    CPC classification number: C12Q1/686 ,  C12Q1/6804 ,  C12Q1/6813 ,  C12Q1/6876 ,  C12Q2600/118 ,  G01N33/53 ,  G01N33/6845 ,  G01N2333/435 ,  G01N2458/10

    Abstract: The invention provides an assay method for detection and/or quantification of a plurality of nucleic acid or protein targets in a sample. In the method probes are used to associate a detectable tag sequence with each of the selected targets present in the sample. Probes or primers sufficient to identify at least 25, and preferably at least 500, different targets are used. The method involves segregating aliquots of the sample from each other and detecting the tag sequences in each aliquot.

    Abstract translation: 本发明提供了用于检测和/或定量样品中多个核酸或蛋白质靶标的测定方法。 在该方法中，探针用于将可检测标签序列与存在于样品中的每个选定靶标相关联。 使用足以鉴定至少25个，优选至少500个不同靶标的探针或引物。 该方法包括将样品的等分试样彼此分离并检测每个等分试样中的标签序列。

    MULTIFUNCTIONAL PROBE-PRIMERS
    92.
    发明申请
    MULTIFUNCTIONAL PROBE-PRIMERS 审中-公开
    多功能探测器

    公开(公告)号：US20140315197A1

    公开(公告)日：2014-10-23

    申请号：US14184600

    申请日：2014-02-19

    Applicant: Fluidigm Corporation

    Inventor： Kenneth J. Livak

    IPC: C12Q1/68

    CPC classification number: C12Q1/6876 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q2525/161 ,  C12Q2525/197 ,  C12Q2565/1015

    Abstract: Methods and reagents for detection and analysis of nucleic acids are provided. Certain methods involves an encoding amplification in which a target sequence is associated with probe-binding sequences and optionally with indexing sequences, (2) an optional distribution step in which the product of the encoding amplification is split into multiple aliquots, and (3) a decoding and detection step in which the presence, absence, quantity, or relative amount of the target sequence in the aliquots is determined. The detection step makes use of a multifunctional “self-digesting” molecular probe comprising a primer polynucleotide and a probe oligonucleotide, linked in a 5′-5′ orientation.

    Abstract translation: 提供了用于检测和分析核酸的方法和试剂。 某些方法涉及编码扩增，其中靶序列与探针结合序列和任选的索引序列相关，（2）任选的分布步骤，其中编码扩增的产物被分成多个等分试样，和（3）a 解码和检测步骤，其中确定等分试样中靶序列的存在，不存在，数量或相对量。 检测步骤使用包含以5'-5'取向连接的引物多核苷酸和探针寡核苷酸的多功能“自消化”分子探针。

    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS
    96.
    发明申请
    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS 有权
    颗粒人口的单粒子分析

    公开(公告)号：US20130323732A1

    公开(公告)日：2013-12-05

    申请号：US13899397

    申请日：2013-05-21

    Applicant: Fluidigm Corporation

    Inventor： Megan Anderson ,  Peilin Chen ,  Brian Fowler ,  Fiona Kaper ,  Ronald Lebofsky ,  Andrew May

    IPC: C12Q1/68 ,  B01D15/08

    CPC classification number: C12Q1/6813 ,  B01D15/08 ,  C12Q1/6806 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out “in parallel,” i.e., essentially simultaneously in the separate reaction volumes.

    Abstract translation: 在某些实施方案中，本发明提供用于测定颗粒群体中的单个颗粒的方法和装置，其中针对每个颗粒测量至少两个参数。 可以在颗粒处于分开的反应体积中时测量一个或多个参数。 或者或另外，可以在稍后的分析步骤中测量一个或多个参数，例如其中在分开的反应体积中进行反应并回收和分析反应产物。 在具体实施方案中，一个或多个参数测量“并行地进行”，即基本上同时在单独的反应体积中进行。

    Microfabricated fluidic circuit elements and applications
    99.
    发明申请
    Microfabricated fluidic circuit elements and applications 有权
    微型流体回路元件及应用

    公开(公告)号：US20020195152A1

    公开(公告)日：2002-12-26

    申请号：US09995397

    申请日：2001-11-26

    Applicant: Fluidigm Corporation

    Inventor： David N. Fernandes ,  Hou-Pu Chou ,  Marc A. Unger

    IPC: F15C001/00

    CPC classification number: F15C3/00 ,  A61M2206/22 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0621 ,  B01L2200/14 ,  B01L2300/123 ,  B01L2400/0487 ,  B01L2400/0638 ,  B01L2400/0655 ,  F15C3/04 ,  F16K99/0001 ,  F16K99/0009 ,  F16K99/0015 ,  F16K99/0034 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0082 ,  F16K2099/0084 ,  G06F17/509 ,  G06F2217/16 ,  H01H2029/008 ,  Y10T137/206 ,  Y10T137/2202 ,  Y10T137/2218 ,  Y10T137/2224 ,  Y10T137/7879 ,  Y10T137/7891 ,  Y10T137/7892

    Abstract: The present invention provides microfabricated fluidic systems and methods. Microfabricated fluidic devices of the present invention include switches that can be opened and closed to allow or block the flow of fluid through a channel in response to the pressure level in a gate of the switch. The microfabricated fluidic switches may be coupled together to perform logic functions and Boolean algebra, such as inverters, AND gates, NAND, gates, NOR gates, and OR gates. The logic gates may be coupled together to form flip-flops that latch signals. The present invention also includes microfabricated fluidic pressure multipliers that increase the pressure in a second chamber relative to a first chamber. Microfabricated fluidic devices of the present invention also include pressure sources. A pressure source of the present includes a pump coupled to a reservoir through unidirectional valves. The pressure source may be high pressure source or a low pressure source. Microfabricated fluidic devices of the present invention may also include devices that perform analog functions such as switching regulator.

    Abstract translation: 本发明提供微制造流体系统和方法。 本发明的微型流体装置包括可以打开和关闭的开关，以响应于开关的门中的压力水平来允许或阻止流体通过通道的流动。 微制造的流体开关可以耦合在一起以执行逻辑功能和布尔代数，例如反相器，与门，NAND，门，NOR门和或门。 逻辑门可以耦合在一起以形成锁存信号的触发器。 本发明还包括相对于第一腔室增加第二腔室中的压力的​​微制造流体压力倍增器。 本发明的微型流体装置还包括压力源。 本发明的压力源包括通过单向阀联接到储存器的泵。 压力源可以是高压源或低压源。 本发明的微型流体装置还可以包括执行诸如开关调节器的模拟功能的装置。

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