公开(公告)号：EP2427544A1

公开(公告)日：2012-03-14

申请号：EP10772921.2

申请日：2010-05-07

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  PHARKYA, Priti

IPC: C12N1/21 ,  C12P13/04 ,  C12P7/44

CPC classification number: C12P17/10 ,  C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/12

Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

公开(公告)号：EP2304039A1

公开(公告)日：2011-04-06

申请号：EP09767691.0

申请日：2009-06-17

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  BURGARD, Anthony, P. ,  PHARKYA, Priti

IPC: C12P5/02 ,  C12P7/42

CPC classification number: C12P7/46 ,  C12N9/88 ,  C12P7/40

Abstract: A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.

公开(公告)号：EP2285973A2

公开(公告)日：2011-02-23

申请号：EP09739876.2

申请日：2009-04-30

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  PHARKYA, Priti

IPC: C12P7/52 ,  C12N1/00 ,  C12P7/40

CPC classification number: C12P7/42 ,  C12N9/0008 ,  C12N9/1096 ,  C12N9/88 ,  C12N9/90 ,  C12P7/40 ,  C12Y102/01 ,  C12Y206/01022 ,  C12Y403/01 ,  C12Y504/99002

Abstract: The invention provides a non-naturally occurring microbial organism having a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzyme in a sufficient amount and culturing under conditions and for a sufficient period of time to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid.

公开(公告)号：EP2262901A1

公开(公告)日：2010-12-22

申请号：EP09717987.3

申请日：2009-03-05

Applicant: Genomatica, Inc.

Inventor： SUN, Jun ,  PHARKYA, Priti ,  BURGARD, Anthony, P.

IPC: C12P7/02 ,  C12N9/00 ,  C12N1/00

CPC classification number: C12P7/04 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/52 ,  C12P7/02 ,  C12Y101/01001 ,  C12Y101/01035 ,  C12Y102/0105 ,  C12Y203/01009 ,  C12Y203/01016 ,  C12Y402/01017

Abstract: The invention provides a non-naturally occurring microbial organism having a microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms.

公开(公告)号：EP4414451A2

公开(公告)日：2024-08-14

申请号：EP24169292.0

申请日：2014-04-25

Applicant: Genomatica, Inc.

Inventor： PHARKYA, Priti ,  BURGARD, Anthony P. ,  VAN DIEN, Stephen J. ,  OSTERHOUT, Robin E. ,  BURK, Mark J. ,  TRAWICK, John D. ,  KUCHINSKAS, Michael P. ,  STEER, Brian ,  ANDRAE, Stefan ,  SHAH, Amit

IPC: C12N15/00

CPC classification number: C12P7/24 ,  C12P7/62 ,  C12P17/04 ,  C12N15/52 ,  C12P7/42 ,  C12P13/005 ,  C12P7/18 ,  C12P13/001 ,  C07D307/33 ,  C12N9/13 ,  C12Y208/03

Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine or related products using the microbial organisms.

公开(公告)号：EP3967747A1

公开(公告)日：2022-03-16

申请号：EP21187599.2

申请日：2014-11-25

Applicant: Genomatica, Inc.

Inventor： OSTERHOUT, Robin E. ,  BURGARD, Anthony P. ,  PHARKYA, Priti ,  ANDREA, Stefan

IPC: C12N1/19 ,  C12N1/15 ,  C12N1/13 ,  C12N1/11 ,  C12N15/70 ,  C12N15/81 ,  C12P7/42 ,  C12P5/02 ,  C12P13/00 ,  C12P17/10 ,  C12N1/14 ,  C12N1/20 ,  C12P7/02 ,  C12P7/40 ,  C12P7/04 ,  C12P7/44 ,  C12P7/18

Abstract: The invention provides non-naturally occurring microbial organisms containing enzymatic pathways and/or metabol- ic modifications for enhancing carbon flux through acetyl-CoA. In some embodiments, the microbial organisms having such path - ways also include pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation. The enhanced carbon flux through acetyl-CoA, in combination with pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation can, in some embodiments, be used for production of a bioderived compound. Accordingly, in some embodi - ments, the microbial organisms of the invention can include a pathway capable of producing a bioderived compound of the inven - tion. The invention still further provides a bioderived compound produced by a microbial organism of the invention, culture medium having the bioderived compound of the invention, compositions having the bioderived compound of the invention, a biobased product comprising the bioderived compound of the invention, and a process for producing a bioderived compound of the invention.

公开(公告)号：EP3792352A2

公开(公告)日：2021-03-17

申请号：EP20184529.4

申请日：2013-08-27

Applicant: Genomatica, Inc.

Inventor： BURGARD, Anthony P. ,  OSTERHOUT, Robin E. ,  VAN DIEN, Stephen J. ,  TRACEWELL, Cara Ann ,  PHARKYA, Priti ,  ANDRAE, Stefan

IPC: C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N9/88 ,  C12N1/21 ,  C12P7/18

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway (MMP) that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.

公开(公告)号：EP3744830A1

公开(公告)日：2020-12-02

申请号：EP20170898.9

申请日：2014-12-23

Applicant: Genomatica, Inc.

Inventor： BURGARD, Anthony P. ,  OSTERHOUT, Robin E. ,  VAN DIEN, Stephen J. ,  PHARKYA, Priti ,  YANG, Tae Hoon ,  CHOI, Jungik

IPC: C12N1/21 ,  C12P7/52 ,  C12P1/04 ,  C12P7/16

Abstract: The invention is directed to a non-naturally occurring microbial organism comprising a first attenuation of a succinyl-CoA synthetase or transferase and at least a second attenuation of a succinyl-CoA converting enzyme or a gene encoding a succinate producing enzyme within a multi-step pathway having a net conversion of succinyl-CoA to succinate.

公开(公告)号：EP3741865A1

公开(公告)日：2020-11-25

申请号：EP20159174.0

申请日：2015-09-18

Applicant: Genomatica, Inc.

Inventor： PHARKYA, Priti ,  BURGARD, Anthony P. ,  NUNEZ VAN NAME, Eric Roland

IPC: C12P7/42 ,  C12P7/16

Abstract: The invention provides non-natural microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA, or oxaloacetate and acetyl-CoA. Embodiments of the invention include microbial organisms having a pathway to acetyl-CoA and oxaloacetate that includes phosphoketolase (a PK pathway). The organisms also have either (i) a genetic modification that enhances the activity of the non-phosphotransferase system (non-PTS) for sugar uptake, and/or (ii) a genetic modification(s) to the organism's electron transport chain (ETC) that enhances efficiency of ATP production, that enhances availability of reducing equivalents or both. The microbial organisms can optionally include (iii) a genetic modification that maintains, attenuates, or eliminates the activity of a phosphotransferase system (PTS) for sugar uptake. The enhanced carbon flux through acetyl-CoA and oxaloacetate can be used for production of a bioderived compound, and the microbial organisms can further include a pathway capable of producing the bioderived compound.

公开(公告)号：EP3280808B1

公开(公告)日：2020-09-16

申请号：EP16718940.6

申请日：2016-04-07

Applicant: Genomatica, Inc.

Inventor： CULLER, Stephanie J. ,  EAKLEY, Nicholas ,  HOFF, Kevin Gregory ,  OSTERHOUT, Robin E. ,  PHARKYA, Priti ,  RALPH, Erik C. ,  VAN DIEN, Stephen

IPC: C12P7/04 ,  C12N1/20 ,  C12N9/02