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    MICROORGANISMS AND METHODS FOR IMPROVING PRODUCT YIELDS ON METHANOL USING ACETYL-COA SYNTHESIS
    105.
    发明公开
    MICROORGANISMS AND METHODS FOR IMPROVING PRODUCT YIELDS ON METHANOL USING ACETYL-COA SYNTHESIS 审中-公开
    微生物与一种提高甲醇由乙酰辅酶A合成的产量

    公开(公告)号：EP3077501A1

    公开(公告)日：2016-10-12

    申请号：EP14868117.4

    申请日：2014-11-25

    Applicant: Genomatica, Inc.

    Inventor： OSTERHOUT, Robin E. ,  BURGARD, Anthony P. ,  PHARKYA, Priti ,  ANDRAE, Stefan

    IPC: C12N1/19 ,  C12N1/15 ,  C12N1/13 ,  C12N1/11

    CPC classification number: C12P5/026 ,  C12N1/14 ,  C12N1/20 ,  C12N15/70 ,  C12N15/81 ,  C12P7/02 ,  C12P7/04 ,  C12P7/18 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P17/10 ,  Y02E50/343

    Abstract: The invention provides non-naturally occurring microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA. In some embodiments, the microbial organisms having such pathways also include pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation. The enhanced carbon flux through acetyl-CoA, in combination with pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation can, in some embodiments, be used for production of a bioderived compound. Accordingly, in some embodiments, the microbial organisms of the invention can include a pathway capable of producing a bioderived compound of the invention. The invention still further provides a bioderived compound produced by a microbial organism of the invention, culture medium having the bioderived compound of the invention, compositions having the bioderived compound of the invention, a biobased product comprising the bioderived compound of the invention, and a process for producing a bioderived compound of the invention.

    Abstract translation: 本发明提供非天然存在的微生物有机体包含酶途径和/或代谢修饰通过乙酰-CoA提高碳通量。 在一些实施方案中，具有检测途径中的微生物生物体因此包括用于产生还原当量，甲醛固定和/或格式同化途径。 通过乙酰辅酶A的增强的碳通量，结合途径产生还原当量，甲醛固定和/或格式可同化，在一些实施方案中，用于生产生物衍生的化合物。 因此，在一些实施方案中，本发明的微生物有机体可包括能够产生本发明的生物衍生化合物的途径。 本发明关进一步提供通过本发明的微生物生物体产生的生物衍生的化合物，具有本发明的生物来源的化合物的培养基，具有本发明的生物衍生的化合物，生物基产品包含本发明的生物衍生的化合物，和处理的组合物 用于生产本发明的生物衍生化合物。

    ALCOHOL DEHYDROGENASE VARIANTS
    106.
    发明公开
    ALCOHOL DEHYDROGENASE VARIANTS 审中-公开
    醇脱氢酶变体

    公开(公告)号：EP3052635A2

    公开(公告)日：2016-08-10

    申请号：EP14850119.0

    申请日：2014-10-03

    Applicant: Genomatica, Inc.

    Inventor： ANDRAE, Stefan ,  KUCHINSKAS, Michael Patrick ,  LI, Jingyi ,  NAGARAJAN, Harish ,  PHARKYA, Priti

    IPC: C12P1/00 ,  C12P5/00

    CPC classification number: C12N9/0006 ,  C12P7/18 ,  C12P7/24 ,  C12Y101/01001 ,  C12Y101/01244 ,  Y02P20/127

    Abstract: Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD
    + -dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

    Abstract translation: 与未修饰的对应物相比，本文描述的是非天然NAD +依赖性醇脱氢酶（ADH），其分别能够将甲醇或乙醇分别转化为甲醛或乙醛的两倍以上。 描述了编码非天然醇脱氢酶的核酸，以及包括核酸的表达构建体，以及包含核酸或表达构建体的工程细胞。 还描述了表达非天然NAD +依赖性醇脱氢酶的工程细胞，任选地包括一种或多种另外的代谢途径转基因，甲醇代谢途径基因，靶产物途径基因，包括细胞的细胞培养组合物，用于促进 从细胞生产目标产物或其中间体，包括目标产物或中间体的组合物，以及由目标产物或中间体制备的产物。

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