公开(公告)号：WO1983002006A1

公开(公告)日：1983-06-09

申请号：PCT/US1982001704

申请日：1982-12-03

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  STEPHENS, Donald, E. ,  EHRET, Robert, J.

IPC: G01N31/08

CPC classification number: G01N30/30 ,  G01N2030/3007 ,  G01N2030/303 ,  G01N2030/8818 ,  G05D23/1919 ,  G05D23/24

Abstract: Un système de commande de la température est utilisé pour commander de manière précise la température d'un courant fluide dans un système analytique conçu pour l'analyse des constituants chimiques du courant fluide. Un exemple d'utilisation en chromatographie est la colonne chromatographique dans un système d'analyse des acides aminés. Le système de commande de température utilise un ensemble de modules thermoélectriques pour transférer la chaleur entre un bloc thermoconducteur qui tient la colonne chromatographique et un puits thermique à échange d'air qui obtient la chaleur du milieu ambiant ou rejette la chaleur dans le milieu ambiant, en fonction des besoins. Le système de commande effectue la régulation de la température à une vitesse très élevée de manière à réduire au minimum le temps de recyclage de l'instrument.

公开(公告)号：WO1998021594A3

公开(公告)日：1998-05-22

申请号：PCT/US1997021332

申请日：1997-11-12

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  FARREN, Carl, A.

IPC: B01L07/00

公开(公告)号：WO1998000717A2

公开(公告)日：1998-01-08

申请号：PCT/US1997011470

申请日：1997-06-30

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  WATTS, Richard ,  GOODALE, David, L. ,  NGO, Dang, M. ,  TU, Songtai ,  TRAN, Michael, Q. ,  BELL, Michael, L.

IPC: G01N35/02

CPC classification number: G01N35/00594 ,  G01N35/00693 ,  G01N35/0092 ,  G01N35/025 ,  G01N2035/00277 ,  G01N2035/00534 ,  G01N2035/00752 ,  G01N2035/0093 ,  G01N2035/0437 ,  G01N2035/0448 ,  G01N2035/0451 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114165 ,  Y10T436/114998 ,  Y10T436/115831 ,  Y10T436/119163

Abstract: An automatic chemistry analyzer is provided having a high throughput and a high reliability. The analyzer uses nephelometric and turbimetric analyzers (68) to analyze a wide variety of parameters within liquid samples typically generated in, for example, a large medical testing laboratory. The machine employs a unique probe (84) and stirring rod (86) assembly (80) mounted at a slight angle ( alpha ) with one another using rack and pinion assemblies (106) so that the lower end of the probe (84) and the lower end of the stirring rod (86) are in very close proximity to one another. This feature allows the machine to be used in unusually small reaction cuvettes (54). The analysis machine also includes an onboard control sample (78) so that the machine can be programmed to periodically calibrate its analyzing equipment during the course of normal operation. The machine also includes a sample station carousel (24) having retainer clips (39) for retaining a sample container rack (30) which is constructed to retain a bar-coded card (37) containing information regarding reagents used in the machine. A bar code reader (47) located proximate to the sample carousel reads the bar-coded reagent information into the controller. Also provided are a cuvette wash station (130) and a waste trap assembly (138).

Abstract translation: 提供了具有高通量和高可靠性的自动化学分析仪。 分析仪使用比浊法和血液分析仪（68）分析通常在大型医学检测实验室产生的液体样品中的各种参数。 机器采用独特的探针（84）和搅拌棒（86）组件（80），其使用齿条和小齿轮组件（106）以彼此以小角度（α）安装，使得探头（84）的下端和 搅拌棒（86）的下端彼此非常接近。 该功能允许机器在异常小的反应池中使用（54）。 分析机还包括车载控制样品（78），使得机器可以被编程为在正常操作过程中周期性地校准其分析设备。 该机器还包括具有用于保持样品容器架（30）的保持夹（39）的样品台转盘（24），该样品容器架（30）构造成保持包含关于在机器中使用的试剂的信息的条形码卡（37）。 位于样品转盘附近的条形码阅读器（47）将条形码试剂信息读入控制器。 还提供了比色皿洗涤台（130）和废物捕集器组件（138）。

公开(公告)号：WO1997010055A1

公开(公告)日：1997-03-20

申请号：PCT/US1996013668

申请日：1996-08-23

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  SASAKI, Glenn, C. ,  HANAMOTO, Barry, K.

IPC: B01L03/00

CPC classification number: B01L3/50255 ,  B01D61/18 ,  G01N35/0099

Abstract: The invention provides a vacuum manifold which can be conveniently used in semi-automated and fully-automated laboratory machines to carry out a sequence of vacuum-assisted laboratory manipulations on a large number of individual liquid samples. The invention has a base (12) which is sized and dimensioned to accept and retain a first liquid receptacle (24), such as a microtitre dish. Generally, the base (12) has a bottom wall (16) and side walls (18) and the first receptacle (24) is designed to be retained within the side walls (18). The invention also has an adapter frame which is sized and dimensioned to accept and retain a second liquid receptacle (30), such as a second microtitre dish. The adapter frame (14) is typically a rectangular frame structure having a lip (32) which supports the second receptacle (30). The base (12) generally has a port (26) which is attachable to a source of vacuum. In operation, the first liquid receptacle (24) is placed within the base (12), the adapter frame (14) is placed on top of the base (12) and the second liquid receptacle (30) is placed on the adapter frame (14) so that a second receptacle (30) is located directly above the first receptacle (24). When vacuum is applied to the base using the vacuum port (26), a uniform vacuum is drawn along the bottom of the second liquid receptacle (30) which provides a driving force which acts upon liquid within the second receptacle (30). In a typical operation, the bottom of the second receptacle (30) is a filtration membrane and the vacuum is used to draw liquid from the second receptacle (30) across the filtered membrane into the first receptacle (24). Because of its modular construction, the various elements of the vacuum frame are easily stacked in stacking frames disposed to one side of the vacuum manifold. This stacking capability allows a large number of vacuum-assisted laboratory operations to be carried out on multiple sets of liquid samples without the necessity of a large amount of horizontal lab bench area. The stacking capability also facilitates the adaption of the invention with robotic equipment to provide a fully-automated laboratory processing tool.

Abstract translation: 本发明提供一种真空歧管，其可以方便地用于半自动化和全自动化的实验室机器中，以在大量单独的液体样品上执行一系列真空辅助实验室操作。 本发明具有基座（12），其尺寸和尺寸被设计成接纳和保持第一液体容器（24），例如微量滴定皿。 通常，底座（12）具有底壁（16）和侧壁（18），并且第一插座（24）被设计成保持在侧壁（18）内。 本发明还具有适配器框架，其尺寸和尺寸适于接纳和保持第二液体容器（30），例如第二微孔板。 适配器框架（14）通常是具有支撑第二容器（30）的唇缘（32）的矩形框架结构。 底座（12）通常具有可连接到真空源的端口（26）。 在操作中，第一液体容器（24）被放置在基座（12）内，适配器框架（14）被放置在基座（12）的顶部上，并且第二液体容器（30）被放置在适配器框架 14），使得第二容器（30）位于第一容器（24）的正上方。 当使用真空端口（26）将真空施加到基座时，沿着第二液体容器（30）的底部抽取均匀的真空，其提供作用于第二容器（30）内的液体的驱动力。 在典型的操作中，第二容器（30）的底部是过滤膜，并且真空用于将来自第二容器（30）的液体穿过过滤的膜抽入第一容器（24）。 由于其模块化构造，真空框架的各种元件容易堆叠在设置在真空歧管一侧的堆叠框架中。 这种堆叠能力允许在多组液体样品上进行大量的真空辅助实验室操作，而不需要大量的水平实验室工作台面积。 堆叠能力还有助于本发明与机器人设备的适应，以提供全自动化的实验室处理工具。

公开(公告)号：WO1997007391A1

公开(公告)日：1997-02-27

申请号：PCT/US1996013139

申请日：1996-08-12

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  MC NEAL, Jack

IPC: G01N21/05

CPC classification number: B01L3/021 ,  G01N21/05 ,  G01N35/00603 ,  G01N2021/054 ,  G01N2035/1018 ,  G01N2035/1062

Abstract: A serum index sample probe having a cuvette (17) with a cylindrical channel (39) coupled between a hollow cylindrical member (25) and a hollow cylindrical needle (23) by an optics block (13). The channel and the needle have matching diameters and are coaxially aligned along an axis (41), defining a smooth bore therebetween to reduce dead volumes. The cuvette is positioned proximate to a puncturing tip of the needle which, along with the smooth bore, reduces turbulence and bubbles in the flow path. The optics block (13) provides a plurality of optical paths to the cuvette while maintaining a linear flow path. The optics block is a manifold having a central aperture (15), a perimeter surface (61) disposed concentrically about the central aperture, and a plurality of bore holes (55, 57, 59).

Abstract translation: 一种血清指数样本探针，其具有通过光学块（13）联接在中空圆柱形构件（25）和中空圆柱形针（23）之间的圆柱形通道（39）的比色杯（17）。 通道和针具有匹配的直径并且沿轴线（41）同轴对准，在其间限定了平滑的孔以减少死体积。 该比色杯定位成靠近针的穿刺尖端，其与光滑孔一起减少流动路径中的湍流和气泡。 光学块（13）在保持线性流动路径的同时提供多个到比色皿的光路。 光学块是具有中心孔（15），围绕中心孔同心设置的周边表面（61）和多个钻孔（55,57,59）的歧管。

公开(公告)号：WO1996006853A1

公开(公告)日：1996-03-07

申请号：PCT/US1995010609

申请日：1995-08-18

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  REDDY, M., Parameswara ,  FAROOQUI, Firdous

IPC: C07H19/10

CPC classification number: C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  Y02P20/55

Abstract: Activated nucleoside derivatives formed in situ of general formula (I) in which one of R and R is R and the other is -P(R2)OR , wherein R is a substituted arylcarbonyl group, R is R O or R , R is a hydroxyl-protecting group and B is a purine or pyrimidine base. Particularly preferred are those compounds wherein R is 2,4-dinitrophenylcarbonyl. The compounds of general formula (I) are prepared using the corresponding carboxylic acids; these acids are generally more soluble in acetonitrile (for example, to the extent of 1.5 M for 2,4-dinitrobenzoic acid) and work as activators at lower concentrations. The compounds of general formula (I) may be employed in conventional coupling reactions (for example, solid phase synthesis) to prepare oligonucleotides which are indistinguishable from those prepared using the heretofore-known tetrazole-activated nucleoside intermediates.

Abstract translation: 活性核苷衍生物原位形成的通式（I）其中R A和R B之一是R 3，另一个是-P（R 2）OR 1，其中R 1是 取代的芳基羰基，R 2是R 4 O或R 5，R 3是羟基保护基，B是嘌呤或嘧啶碱基。 特别优选的是其中R 1是2,4-二硝基苯基羰基的那些化合物。 通式（I）的化合物使用相应的羧酸制备; 这些酸通常在乙腈中更易溶（例如对于2,4-二硝基苯甲酸为1.5M的程度），作为较低浓度的活化剂。 通式（I）的化合物可以用于常规偶合反应（例如固相合成）中，以制备与使用迄今为止已知的四唑活化的核苷中间体制备的寡核苷酸不同的寡核苷酸。

公开(公告)号：WO1996001696A1

公开(公告)日：1996-01-25

申请号：PCT/US1995008508

申请日：1995-07-07

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  SHARPLES, Thomas, D.

IPC: B04B13/00

CPC classification number: B04B13/003 ,  B04B15/02

Abstract: A method and system of identifying a rotor of a centrifuge employ an approach that uses two tiers of model selection. Firstly, the moment of inertia of a rotor is calculated for a first measured acceleration. The indication of moment of inertia is utilized to disqualify a number of rotor models and to select a subset of models. In a second tier, windage power of the rotor is calculated in a manner that isolates windage from inertial drag. In one embodiment, drive torque is measured with the rotor operated at a high constant speed. Alternatively, windage is calculated using data obtained during a second measured acceleration. The accuracy of the computation is enhanced by taking into account the moment of inertia as one form of resistance to the second acceleration. Based upon the indication of windage power, at least one rotor model within the subset is disqualified. Upon identification of the rotor, the centrifugal process can be maintained at a maximum safe speed. Moreover, a refrigeration offset circuit is controlled to provide a dynamic temperature correction with changes in windage power.

Abstract translation: 识别离心机转子的方法和系统采用使用两层模型选择的方法。 首先，对于第一测量加速度计算转子的惯性矩。 惯性矩的指示用于取消多个转子模型的资格，并选择一个模型子集。 在第二层中，转子的风力功率是以将惯性阻力隔离的方式进行计算的。 在一个实施例中，用转子以高恒定速度运行来测量驱动转矩。 或者，使用在第二测量加速度期间获得的数据计算风量。 通过将惯性矩考虑为对第二加速度的一种阻力的形式来增强计算的精度。 基于风力功率的指示，该子集内的至少一个转子模型被取消资格。 在识别转子时，可以将离心过程保持在最大安全速度。 此外，制冷补偿电路被控制以提供具有风力功率变化的动态温度校正。

公开(公告)号：WO1995028637A1

公开(公告)日：1995-10-26

申请号：PCT/US1995004433

申请日：1995-04-12

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  CHEN, Fu-Tai, Albert

IPC: G01N27/447

CPC classification number: C12Q1/00 ,  C12Q1/62 ,  G01N27/44721 ,  G01N27/44747 ,  Y10T436/103332

Abstract: A method for detecting an analyte in a sample uses both the specificity of an enzymatic reaction and the separation power of capillary electrophoresis. In general, the method comprises: (1) subjecting a first aliquot of the sample to an analytical technique such as capillary electrophoresis, which generates a first output such as an electropherogram; (2) reacting a second aliquot of the sample in an enzyme-catalyzed reaction converting the analyte into a product, the product being detectable by the analytical technique; (3) subjecting the second aliquot to the analytical technique to generate a second output; (4) in the case of electrophoresis, measuring the absorbance of the first and second outputs (electropherograms) as a function of migration distance along the electropherogram at at least one wavelength at which either the analyte or the product absorbs to produce a first absorbance scan and a second absorbance scan; and (5) comparing the first absorbance scan with the second absorbance scan to detect the analyte. The reaction can involve a coenzyme and the analytical technqiue can be directed to the coenzyme. Alternatively, at least two enzymes can be used, the first enzyme generating a first product that is then acted upon by the second enzyme.

Abstract translation: 用于检测样品中的分析物的方法使用酶反应的特异性和毛细管电泳的分离能力。 通常，该方法包括：（1）对样品的第一等分试样进行诸如毛细管电泳的分析技术，其产生诸如电泳图的第一输出; （2）使样品的第二等分试样在酶分析反应中转化为产物，该产物可通过分析技术检测; （3）对第二等分试样进行分析技术以产生第二输出; （4）在电泳的情况下，测量第一和第二输出（电泳图）的吸光度，作为在分析物或产物吸收的至少一个波长处沿着电泳图的迁移距离的函数，以产生第一吸光度扫描 和第二吸光度扫描; 和（5）将第一吸光度扫描与第二吸光度扫描进行比较以检测分析物。 该反应可以涉及辅酶，分析技术可以涉及辅酶。 或者，可以使用至少两种酶，第一种酶产生随后被第二种酶作用的第一种产物。

公开(公告)号：WO1995011912A2

公开(公告)日：1995-05-04

申请号：PCT/US1994012437

申请日：1994-10-28

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  COASSIN, Peter, J. ,  MATSON, Robert, S. ,  RAMPAL, Jang, B.

IPC: C07H21/00

CPC classification number: C07H21/00 ,  B01J19/0046 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00731 ,  C07K1/042 ,  C12Q1/6834 ,  C12Q1/6869 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  G01N33/54353 ,  Y10T436/143333

Abstract: Disclosed herein are surface activated, organic polymers useful for biopolymer synthesis. Most preferably, aminated biaxially oriented polypropylene is used for the synthesis of oligonucleotides thereto, and these devices are most preferably utilized for genetic analysis of patient samples.

Abstract translation: 本文公开了可用于生物聚合物合成的表面活化的有机聚合物。 最优选地，胺化双轴取向聚丙烯用于合成寡核苷酸，并且这些装置最优选用于患者样品的遗传分析。

公开(公告)号：WO1995009176A2

公开(公告)日：1995-04-06

申请号：PCT/US1993009294

申请日：1993-09-28

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  COASSIN, Peter, J. ,  MATSON, Robert, S. ,  RAMPAL, Jang, B.

IPC: C07H21/00