公开(公告)号：US06399385B1

公开(公告)日：2002-06-04

申请号：US09670277

申请日：2000-09-27

Applicant: Maria Croyle ,  James M. Wilson

Inventor： Maria Croyle ,  James M. Wilson

IPC: C12N15861

CPC classification number: C12N7/00 ,  A61K47/6901 ,  A61K48/00 ,  C12N2710/10041 ,  C12N2710/10043 ,  C12N2710/10051 ,  C12N2750/14143 ,  C12N2750/14151

Abstract: A rapid method for modifying a viral capsid or envelope protein with a polyethylene glycol (PEG) is described. Also provided are methods of delivering a molecule using the PEG-modified viruses of the invention. Compositions containing the PEG-modified viruses of the invention, are characterized by improved gene expression, reduced neutralizing antibody and CTL production. Also provided are viral compositions having enhanced physical stability, in which the viruses are lyophilized in a formulation having a 1:1 ratio of sucrose and mannitol are provided.

Abstract translation: 描述了用聚乙二醇（PEG）修饰病毒衣壳或包膜蛋白的快速方法。 还提供了使用本发明的PEG修饰的病毒递送分子的方法。 含有本发明PEG修饰病毒的组合物的特征在于改善基因表达，降低中和抗体和CTL产生。 还提供了具有增强的物理稳定性的病毒组合物，其中病毒在具有1:1比例的蔗糖和甘露糖醇的制剂中冻干。

公开(公告)号：US06399370B1

公开(公告)日：2002-06-04

申请号：US09228302

申请日：1999-01-12

Applicant: James M. Wilson ,  Mitchell Goldman ,  Robert Bals ,  Ethan D. Stolzenberg ,  Mark Anderson ,  Michael Zasloff ,  Prasad Kari

Inventor： James M. Wilson ,  Mitchell Goldman ,  Robert Bals ,  Ethan D. Stolzenberg ,  Mark Anderson ,  Michael Zasloff ,  Prasad Kari

IPC: C07H2100

CPC classification number: C07K14/4723 ,  A61K38/00

Abstract: The invention relates to mammalian beta defensin and methods of use thereof for treatment of microbial infection.

Abstract translation: 本发明涉及哺乳动物β防御素及其用于治疗微生物感染的方法。

公开(公告)号：US06365394B1

公开(公告)日：2002-04-02

申请号：US09659203

申请日：2000-09-11

Applicant: Guangping Gao ,  James M. Wilson

Inventor： Guangping Gao ,  James M. Wilson

IPC: C12N700

CPC classification number: C12N7/00 ,  A61K48/00 ,  C07K14/005 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10352

Abstract: Novel cell lines useful for trans-complementing E1-deleted adenoviral vectors are described. The cell lines are capable of providing high yields of E1-deleted adenoviral vectors in the absence of replication-competent adenovirus over multiple passages.

公开(公告)号：US06316255B1

公开(公告)日：2001-11-13

申请号：US08471922

申请日：1995-06-06

Applicant: Richard C. Mulligan ,  James M. Wilson

Inventor： Richard C. Mulligan ,  James M. Wilson

IPC: C12N1500

CPC classification number: C12N15/86 ,  A61K38/00 ,  A61K48/00 ,  C07K14/635 ,  C07K14/705 ,  C12N2710/10243 ,  C12N2740/13043 ,  Y10S435/948

Abstract: Genetically engineered or transduced hepatocytes which express genetic material of interest introduced or incorporated into them, as well as methods of producing, transplanting and using the genetically engineered hepatocytes. The genetic material of interest can be incorporated through use of a vector, such as a recombinant retrovirus, which contains the genetic material of interest, or by other method.

Abstract translation: 表达遗传材料的转基因或转导肝细胞引入或并入其中，以及生产，移植和使用遗传工程化肝细胞的方法。 感兴趣的遗传物质可以通过使用包含感兴趣的遗传物质的重组逆转录病毒载体或其他方法来引入。

公开(公告)号：US06287571B1

公开(公告)日：2001-09-11

申请号：US09347060

申请日：1999-07-02

Applicant: Hildegund C. J. Ertl ,  James M. Wilson

Inventor： Hildegund C. J. Ertl ,  James M. Wilson

IPC: A61K3912

CPC classification number: C07K14/005 ,  A61K2039/53 ,  C12N2710/10343 ,  C12N2760/20134

Abstract: A replication defective recombinant adenovirus is provided which contains a complete deletion of its E1 gene and at least a partial deletion of its E3 gene, said virus containing in the site of the E1 deletion a sequence comprising a non-adenovirus promoter directing the replication and expression of DNA encoding a heterologous protein from a disease-causing agent, which, when administered to a mammal in said recombinant virus, elicits a substantially complete protective immune response against the agent. Pharmaceutical and veterinary products containing the recombinant adenovirus are provided.

Abstract translation: 提供了复制缺陷型重组腺病毒，其包含其E1基因的完全缺失和其E3基因的至少部分缺失，所述病毒在E1缺失位点中含有包含指导复制和表达的非腺病毒启动子的序列 编码来自致病因子的异源蛋白质的DNA，当向所述重组病毒中的哺乳动物施用时，对所述药剂引发基本完全的保护性免疫应答。 提供含有重组腺病毒的药物和兽药产品。

公开(公告)号：US06251677B1

公开(公告)日：2001-06-26

申请号：US09246320

申请日：1999-02-08

Applicant: James M. Wilson ,  William M. Kelley ,  Krishna J. Fisher

Inventor： James M. Wilson ,  William M. Kelley ,  Krishna J. Fisher

IPC: C12N15861

CPC classification number: C12N15/86 ,  A61K48/00 ,  A61K2121/00 ,  C12N7/00 ,  C12N2710/10321 ,  C12N2710/10344 ,  C12N2750/14143

Abstract: The present invention provides a hybrid vector construct which comprises a portion of an adenovirus, 5′ and 3′ ITR sequences from an AAV, and a selected transgene. Also provided is a hybrid virus linked via a polycation conjugate to an AAV rep gene to form a single particle. These trans-infection particles are characterized by high titer transgene delivery to a host cell and the ability to stably integrate the transgene into the host cell chromosome. Also disclosed is the use of the hybrid vectors and viruses to produce large quantities of recombinant AAV.

Abstract translation: 本发明提供了一种杂合载体构建体，其包含一部分腺病毒，来自AAV的5'和3'ITR序列以及选择的转基因。 还提供了通过聚阳离子缀合物与AAV rep基因连接以形成单个颗粒的杂交病毒。 这些反式感染颗粒的特征在于高滴度转移到宿主细胞的转基因，以及将转基因稳定整合到宿主细胞染色体中的能力。 还公开了杂交载体和病毒用于产生大量重组AAV的用途。

公开(公告)号：US6001350A

公开(公告)日：1999-12-14

申请号：US488420

申请日：1995-06-07

Applicant: Richard C. Mulligan ,  Lawrence K. Cohen ,  Louis K. Birinyi ,  James M. Wilson

Inventor： Richard C. Mulligan ,  Lawrence K. Cohen ,  Louis K. Birinyi ,  James M. Wilson

IPC: A61K38/19 ,  A61K38/37 ,  A61K38/46 ,  A61K38/49 ,  A61K48/00 ,  A61L27/38 ,  A61P9/00 ,  C12N15/867 ,  C12N5/10

CPC classification number: A61L27/507 ,  A61K35/44 ,  A61K38/166 ,  A61K38/49 ,  A61L27/3808 ,  C12N15/86 ,  C12Y304/21068 ,  A61K48/00 ,  C12N2740/13043

Abstract: Endothelial cells transduced with genetic material encoding a polypeptide or protein of interest and, optionally, a selectable marker, as well as methods for making and using the transduced endothelial cells are disclosed. Such endothelial cells are useful in improving the performance of vascular grafts and in delivering the encoded polypeptide or protein, such as an enzyme, a hormone, a receptor or a drug, to an individual.

Abstract translation: 公开了用编码目的多肽或蛋白质，任选的选择性标记的遗传物质转导的内皮细胞，以及制备和使用转导的内皮细胞的方法。 这样的内皮细胞可用于改善血管移植物的性能并将编码的多肽或蛋白质例如酶，激素，受体或药物递送给个体。

公开(公告)号：US5856152A

公开(公告)日：1999-01-05

申请号：US331384

申请日：1994-10-28

Applicant: James M. Wilson ,  William M. Kelley ,  Krishna J. Fisher

Inventor： James M. Wilson ,  William M. Kelley ,  Krishna J. Fisher

IPC: C12N15/09 ,  A61K35/76 ,  A61K38/00 ,  A61K48/00 ,  A61P43/00 ,  C07K14/47 ,  C07K14/705 ,  C12N5/10 ,  C12N7/00 ,  C12N15/12 ,  C12N15/861 ,  C12N15/864 ,  C12N15/87 ,  C12N15/10 ,  C12N15/64 ,  C12N15/86

CPC classification number: C12N15/86 ,  C07K14/4712 ,  C07K14/705 ,  C12N15/87 ,  A61K38/00 ,  A61K48/00 ,  C12N2710/10344 ,  C12N2750/14143 ,  C12N2750/14151 ,  C12N2750/14152 ,  C12N2810/40 ,  C12N2830/008

Abstract: The present invention provides a hybrid vector construct which comprises a portion of an adenovirus, 5' and 3' ITR sequences from an AAV, and a selected transgene. Other hybrid vectors form a polycation conjugate and incorporate an AAV rep gene in a single particle. These hybrid virus vectors are characterized by high titer transgene delivery to a host cell and the ability to stably integrate the transgene into the host cell chromosome. Also disclosed is the use of the hybrid vectors to produce large quantities of recombinant AAV.

Abstract translation: 本发明提供了一种杂合载体构建体，其包含一部分腺病毒，来自AAV的5'和3'ITR序列以及选择的转基因。 其他杂合载体形成聚阳离子缀合物并将AAV rep基因并入单个颗粒中。 这些杂种病毒载体的特征在于高滴度转移至宿主细胞的转基因，以及将转基因稳定整合入宿主细胞染色体的能力。 还公开了杂交载体用于产生大量重组AAV的用途。

公开(公告)号：US5667766A

公开(公告)日：1997-09-16

申请号：US486920

申请日：1995-06-07

Applicant: James M. Wilson ,  John F. Engelhardt

Inventor： James M. Wilson ,  John F. Engelhardt

IPC: A01K67/027 ,  A61L27/38 ,  A61K49/00 ,  A01N1/02 ,  A61K35/42 ,  C12N5/00

CPC classification number: A61L27/3813 ,  A01K67/027 ,  A61L27/3604

Abstract: A non-human animal characterized by a human airway, the human airway being open-ended. The human airway is characterized by cells derived from the human respiratory system. The non-human animal and the human airway it carries are useful as models of human airways, particularly diseased human airways, for example, human CF airways. They are also useful to predict the efficacy or toxicity of agents and therapies on human airways, particularly on diseased human airways, for example, human CF airways.

Abstract translation: 以人类气道为特征的非人类动物，人类气道是开放式的。 人类气道的特征在于来自人类呼吸系统的细胞。 携带的非人动物和人类气道可用作人类气道的模型，特别是患病的人类气道，例如人类CF气道。 它们也可用于预测药物和治疗对人类气道的功效或毒性，特别是患病人类气道，例如人类CF气道。

公开(公告)号：US5652224A

公开(公告)日：1997-07-29

申请号：US393734

申请日：1995-02-24

Applicant: James M. Wilson ,  Karen Kozarsky ,  Jerome Strauss, III

Inventor： James M. Wilson ,  Karen Kozarsky ,  Jerome Strauss, III

IPC: C12N15/09 ,  A61K35/76 ,  A61K38/00 ,  A61K38/17 ,  A61K48/00 ,  A61P3/06 ,  C07K14/705 ,  C12N5/10 ,  C12N7/00 ,  C12N15/12 ,  C12N15/861 ,  C12N15/00

CPC classification number: C12N15/86 ,  C07K14/705 ,  A61K38/00 ,  A61K48/00 ,  C12N2710/10344 ,  C12N2799/022

Abstract: The invention provides a recombinant viral vector comprising the DNA of, or corresponding to, at least a portion of the genome of an adenovirus, which portion is capable of infecting a hepatic cell; and a human VLDL receptor gene operatively linked to regulatory sequences directing its expression. The vector is capable of expressing the normal VLDL receptor gene product in hepatic cells in vivo or in vitro. This viral vector is useful in the treatment of metabolic disorders caused by the accumulation of LDL in plasma, such as familial hypercholesterolemia or familial combined hyperlipidemia.

Abstract translation: 本发明提供了一种重组病毒载体，其包含腺病毒基因组的至少一部分的DNA或相当于该部分能够感染肝细胞的DNA; 和与指导其表达的调节序列可操作地连接的人类VLDL受体基因。 该载体能够在体内或体外在肝细胞中表达正常的VLDL受体基因产物。 该病毒载体可用于治疗血浆中LDL积累引起的代谢紊乱，如家族性高胆固醇血症或家族性高脂血症。