公开(公告)号：US20150177150A1

公开(公告)日：2015-06-25

申请号：US14543867

申请日：2014-11-17

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Jason W. Sickler ,  Brett J. Gyarfas ,  Jeremy Lackey ,  Gerard Schmid ,  Benjamin Cipriany ,  Jack Jewell ,  Lawrence West ,  Michael Ferrigno ,  Paul E. Glenn ,  Adam E. Cohen ,  Anthony Bellofiore

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0829 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/168 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2521/101 ,  C12Q2537/157 ,  C12Q2563/107 ,  C12Q2565/607 ,  G01N21/64 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/648 ,  G01N21/7743 ,  G01N21/7746 ,  G01N2021/6419 ,  G01N2021/6441 ,  G01N2021/6478 ,  G01N2201/02 ,  G01N2201/06113 ,  G01N2201/0612 ,  G01N2201/062 ,  G01N2201/068 ,  G01N2201/125 ,  Y10T29/49016 ,  C12Q2525/101

Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.

Abstract translation: 用于分析单分子和进行核酸测序的装置和方法。 装置可以包括测定芯片，其包括多个像素，其中样品阱被配置为接收样品，当被激发时，样品阱发射发射能量; 用于在特定方向上引导发射能量的至少一个元件; 以及发光能量从样品阱向传感器行进的光路。 该装置还包括与测定芯片接口的仪器。 仪器包括用于激发每个样品井中的样品的激发光源; 多个传感器对应于样品孔。 每个传感器可以检测来自相应样品阱中的样品的发射能量。 仪器包括至少一个光学元件，其将来自每个样品的发射能量引向多个传感器中的相应传感器。

公开(公告)号：US20150141268A1

公开(公告)日：2015-05-21

申请号：US14543888

申请日：2014-11-17

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Jason W. Sickler ,  Brett J. Gyarfas ,  Jeremy Lackey ,  Gerard Schmid

IPC: C12Q1/68 ,  G01N21/64

CPC classification number: G01N21/6486 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0829 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/168 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2521/101 ,  C12Q2537/157 ,  C12Q2563/107 ,  C12Q2565/607 ,  G01N21/64 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/648 ,  G01N21/7743 ,  G01N21/7746 ,  G01N2021/6419 ,  G01N2021/6441 ,  G01N2021/6478 ,  G01N2201/02 ,  G01N2201/06113 ,  G01N2201/0612 ,  G01N2201/062 ,  G01N2201/068 ,  G01N2201/125 ,  Y10T29/49016 ,  C12Q2525/101

Abstract: An active-source-pixel, integrated device capable of performing biomolecule detection and/or analysis, such as single-molecule nucleic acid sequencing, is described. An active pixel of the integrated device includes a sample well into which a sample to be analyzed may diffuse, an excitation source for providing excitation energy to the sample well, and a sensor configured to detect emission from the sample. The sensor may comprise two or more segments that produce a set of signals that are analyzed to differentiate between and identify tags that are attached to, or associated with, the sample. Tag differentiation may be spectral and/or temporal based. Identification of the tags may be used to detect, analyze, and/or sequence the biomolecule.

Abstract translation: 描述了能够进行生物分子检测和/或分析的活性源像素集成器件，例如单分子核酸测序。 集成器件的有源像素包括样本阱，待分析的样本可以扩散到其中，用于向样本阱提供激发能量的激发源以及被配置为检测来自样本的发射的传感器。 传感器可以包括产生一组信号的两个或更多个片段，所述一组信号被分析以区分和识别附加到样本或与样本相关联的标签。 标签分化可以是基于频谱和/或时间的。 标签的识别可用于检测，分析和/或序列生物分子。

公开(公告)号：US20150141267A1

公开(公告)日：2015-05-21

申请号：US14543865

申请日：2014-11-17

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Jason W. Sickler ,  Brett J. Gyarfas ,  Jeremy Lackey ,  Gerard Schmid ,  Benjamin Cipriany ,  Jack Jewell ,  Lawrence West ,  Michael Ferrigno ,  Paul E. Glenn ,  Anthony Bellofiore

IPC: C12Q1/68 ,  G01N21/64

CPC classification number: G01N21/6486 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0829 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/168 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2521/101 ,  C12Q2537/157 ,  C12Q2563/107 ,  C12Q2565/607 ,  G01N21/64 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/648 ,  G01N21/7743 ,  G01N21/7746 ,  G01N2021/6419 ,  G01N2021/6441 ,  G01N2021/6478 ,  G01N2201/02 ,  G01N2201/06113 ,  G01N2201/0612 ,  G01N2201/062 ,  G01N2201/068 ,  G01N2201/125 ,  Y10T29/49016 ,  C12Q2525/101

Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits radiation; at least one element for directing the emission radiation in a particular direction; and a light path along which the emission radiation travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the integrated device. Each sensor may detect emission radiation from a sample in a respective sample well. The instrument includes an excitation light source for exciting the sample in each sample well.

Abstract translation: 用于分析单分子和进行核酸测序的装置和方法。 集成器件包括多个像素，其中样品阱被配置为接收样品，当被激发时，样品阱发射辐射; 用于在特定方向上引导发射辐射的至少一个元件; 以及发射辐射从样品阱向传感器行进的光路。 该装置还包括与集成装置接口的仪器。 每个传感器可以检测来自相应样品阱中的样品的发射辐射。 该仪器包括用于激发每个样品孔中的样品的激发光源。

公开(公告)号：US20150069247A1

公开(公告)日：2015-03-12

申请号：US14394616

申请日：2013-04-14

Applicant: NANYANG TECHNOLOGICAL UNIVERSITY

Inventor： Anand Krishna Asundi ,  Chi Seng Ng

IPC: G01N21/95 ,  G01N21/88

CPC classification number: G01N21/9501 ,  G01N21/23 ,  G01N21/8806 ,  G01N21/9505 ,  G01N2021/8848 ,  G01N2201/062 ,  G01N2201/125

Abstract: There is provided a method and system for real time inspection of a silicon wafer. The method includes using an infrared plane polariscope to obtain an image of a bonded interface of the silicon wafer, the image showing stress patterns; and assessment of the stress patterns. The stress patterns in a form of at least one butterfly pattern indicates a presence of at least one of: at least one trapped particle, trapped gases and at least one de-bonding region. No computer/algorithm processing is carried out to locate defects/de-bondings at the bonded interface. Furthermore, the stress fields being generated can be used to approximate the size of the de-bonding region/trapped particle. The system employs the infrared plane polariscope to obtain an image of a bonded interface of the silicon wafer.

Abstract translation: 提供了一种用于硅晶片实时检测的方法和系统。 该方法包括使用红外平面偏振器来获得硅晶片的键合界面的图像，该图像显示出应力模式; 并评估压力模式。 至少一个蝶形图案形式的应力模式表示存在至少一种捕获的颗粒，捕获的气体和至少一个去粘合区域中的至少一种。 不进行计算机/算法处理来定位键合界面处的缺陷/去粘合。 此外，产生的应力场可以用于近似去结合区域/捕获的颗粒的尺寸。 该系统采用红外平面偏振器来获得硅晶片的键合界面的图像。

公开(公告)号：US20150056719A1

公开(公告)日：2015-02-26

申请号：US14313615

申请日：2014-06-24

Applicant: Neven Karlovac ,  Onur Mudanyali ,  Sophie Lorraine Gerrick ,  Ray Carlisle Delcher ,  Derek Kuochao Tseng

Inventor： Neven Karlovac ,  Onur Mudanyali ,  Sophie Lorraine Gerrick ,  Ray Carlisle Delcher ,  Derek Kuochao Tseng

IPC: G01N21/59 ,  H04N7/18 ,  H04N5/235 ,  G01N21/55

CPC classification number: G01N21/59 ,  G01N21/55 ,  G01N21/8483 ,  G01N33/48785 ,  G01N2021/1765 ,  G01N2201/062 ,  G01N2201/125 ,  H04M1/72527 ,  H04M2250/52 ,  H04N5/2354 ,  H04N7/18

Abstract: A universal rapid diagnostics test reader is disclosed and described herein that includes a set of control electronics, a digital camera component, an illumination component, a housing component, and a rapid diagnostics test tray, wherein the tray can hold at least one rapid diagnostics test having a shape and a size in a fixed position relative to the digital camera component and the illumination component, and wherein the reader can accommodate more than one different rapid diagnostics test. Methods are also disclosed that include: providing at least one first rapid diagnostics test having a first physical size, first feature and first format; providing at least one second rapid diagnostics test having a second physical size, second feature and second format; inserting the first rapid diagnostics test in a universal rapid diagnostics test reader; analyzing the first rapid diagnostics test using the universal rapid diagnostics test reader; removing the first rapid diagnostics test from the reader; inserting the second rapid diagnostics test in a universal rapid diagnostics test reader without any mechanical adjustments of the reader or without the use of any additional parts or additional inserts; and analyzing the second rapid diagnostics test using the universal rapid diagnostics test reader.

Abstract translation: 本文公开和描述了一种通用快速诊断测试读取器，其包括一组控制电子设备，数字照相机部件，照明部件，外壳部件和快速诊断测试托盘，其中托盘可以容纳至少一个快速诊断测试 具有相对于数字照相机部件和照明部件的固定位置的形状和尺寸，并且其中读取器可容纳多于一种不同的快速诊断测试。 还公开了包括：提供具有第一物理尺寸，第一特征和第一格式的至少一个第一快速诊断测试的方法; 提供具有第二物理尺寸，第二特征和第二格式的至少一个第二快速诊断测试; 将第一个快速诊断测试插入通用快速诊断测试读取器; 使用通用快速诊断测试阅读器分析第一次快速诊断测试; 从阅读器中删除第一个快速诊断测试; 将第二个快速诊断测试插入通用快速诊断测试读取器，无需读取器的任何机械调整或不使用任何附加部件或附加插入件; 并使用通用快速诊断测试读取器分析第二次快速诊断测试。

公开(公告)号：US08855399B2

公开(公告)日：2014-10-07

申请号：US13368306

申请日：2012-02-07

Applicant: Zvi Goren ,  Nir Ben-David Dodzin

Inventor： Zvi Goren ,  Nir Ben-David Dodzin

IPC: G06K9/00

CPC classification number: G06T7/0008 ,  G01B11/14 ,  G01N21/9501 ,  G01N2201/10 ,  G01N2201/125 ,  G01N2201/127 ,  G06T7/32 ,  G06T2207/30148

Abstract: A system for location based wafer analysis, the system comprising: (i) a first input interface; (ii) a second input interface; (iii) a correlator; and (iv) a processor, configured to generate inspection results for the inspected wafer, with the help of at least one frame run-time displacement.

Abstract translation: 一种用于基于位置的晶片分析的系统，所述系统包括：（i）第一输入接口; （ii）第二输入接口; （iii）相关器; 以及（iv）处理器，被配置为通过至少一个帧运行时位移来产生检查的晶片的检查结果。

公开(公告)号：US08385615B2

公开(公告)日：2013-02-26

申请号：US12390690

申请日：2009-02-23

Applicant: Richard Levenson ,  Paul J. Cronin ,  Kirk William Gossage ,  Clifford C. Hoyt

Inventor： Richard Levenson ,  Paul J. Cronin ,  Kirk William Gossage ,  Clifford C. Hoyt

IPC: G06K9/00 ,  G06K9/46 ,  G06K9/62 ,  G06K9/66 ,  G01N33/48 ,  G01N21/64 ,  G01J3/00 ,  G01J1/00 ,  G01J1/58 ,  G01T1/00 ,  G21G4/00 ,  A61B5/05 ,  A61B6/00

CPC classification number: G01N33/4833 ,  A61B5/0059 ,  B82Y5/00 ,  B82Y10/00 ,  G01J3/32 ,  G01J3/4406 ,  G01J3/453 ,  G01N21/255 ,  G01N21/6486 ,  G01N2201/062 ,  G01N2201/125

Abstract: The invention features a method including: (i) providing spectrally resolved information about light coming from different spatial locations in a sample comprising deep tissue in response to an illumination of the sample, wherein the light includes contributions from different components in the sample; (ii) decomposing the spectrally resolved information for each of at least some of the different spatial locations into contributions from spectral estimates associated with at least some of the components in the sample; and (iii) constructing a deep tissue image of the sample based on the decomposition to preferentially show a selected one of the components.

Abstract translation: 本发明的特征在于一种方法，包括：（i）响应于样品的照明，提供关于来自包含深层组织的样品中的不同空间位置的光的光谱解析信息，其中所述光包括来自样品中不同组分的贡献; （ii）将所述不同空间位置中的至少一些中的每一个的所述光谱解析信息分解为与所述样本中的至少一些组件相关联的频谱估计的贡献; 和（iii）基于分解构建样品的深层组织图像，以优先显示所选择的组分之一。

公开(公告)号：US20100315629A1

公开(公告)日：2010-12-16

申请号：US12724192

申请日：2010-03-15

Applicant: Kevin J. Knopp ,  Robert L. Green ,  Brendon D. Tower ,  Christopher D. Brown ,  Gregory H. Vander Rhodes

Inventor： Kevin J. Knopp ,  Robert L. Green ,  Brendon D. Tower ,  Christopher D. Brown ,  Gregory H. Vander Rhodes

IPC: G01J3/44 ,  G01J3/00 ,  G01N21/62 ,  G01N21/55 ,  G01N21/47 ,  G01N21/59

CPC classification number: G01N21/17 ,  G01J3/02 ,  G01J3/0232 ,  G01J3/0262 ,  G01J3/0264 ,  G01J3/0272 ,  G01J3/0289 ,  G01J3/0291 ,  G01J3/42 ,  G01J3/44 ,  G01J3/4412 ,  G01N21/47 ,  G01N21/4738 ,  G01N21/55 ,  G01N21/59 ,  G01N21/65 ,  G01N2021/3595 ,  G01N2201/0221 ,  G01N2201/0612 ,  G01N2201/062 ,  G01N2201/105 ,  G01N2201/125

Abstract: Disclosed are apparatus, kits, methods, and systems that include a radiation source configured to direct radiation to a sample; a detector configured to measure radiation from the sample; an electronic processor configured to determine infotmation about the sample based on the measured radiation; a housing enclosing the source, the detector, and the electronic processor, the housing having a hand-held form factor; an arm configured to maintain a separation between the sample and the housing, the arm including a first end configured to connect to the housing and a second end configured to contact the sample; and a layer positioned on the second end of the arm, the layer being configured to contact the sample and to transmit at least a portion of the radiation from the sample to the detector.

Abstract translation: 公开了包括被配置为将辐射引导到样品的辐射源的装置，试剂盒，方法和系统; 检测器，被配置为测量来自样品的辐射; 配置为基于所测量的辐射来确定关于样本的信息的电子处理器; 包围所述源，所述检测器和所述电子处理器的外壳，所述外壳具有手持外形尺寸; 臂被配置为保持样品和壳体之间的分离，所述臂包括构造成连接到壳体的第一端和被配置为接触样品的第二端; 以及位于所述臂的第二端上的层，所述层被配置为接触所述样品并将所述辐射的至少一部分从所述样品透射到所述检测器。

公开(公告)号：US20090052770A1

公开(公告)日：2009-02-26

申请号：US11831282

申请日：2007-07-31

Applicant: M. Anthony Lewis ,  Ralph Etienne-Cummings ,  Kaijen Hsiao ,  Ilyas M. Ayub ,  Viktor Gruev ,  Chris F. Milne

Inventor： M. Anthony Lewis ,  Ralph Etienne-Cummings ,  Kaijen Hsiao ,  Ilyas M. Ayub ,  Viktor Gruev ,  Chris F. Milne

IPC: G06K9/00

CPC classification number: G01J3/50 ,  G01D7/12 ,  G01J3/02 ,  G01J3/0262 ,  G01J3/0264 ,  G01J3/0272 ,  G01J3/46 ,  G01J3/463 ,  G01J3/501 ,  G01J3/513 ,  G01J3/526 ,  G01J2003/466 ,  G01N21/255 ,  G01N21/27 ,  G01N2201/12 ,  G01N2201/125 ,  G09B19/0023 ,  G09B21/006

Abstract: A device comprising an illumination means and a light sensing means, that can examine and memorize a discrete color of an object based on the magnitude of the reflected light bouncing off of the colored surface in at least three areas of the electromagnetic spectrum. The device also provides output as a visually and/or audibly perceptible signal for deciphering the color. The color range identified by the device is not limited to the visible spectrum and may include infra-red and ultra-violet light. A storage means for memorizing colors may also be included in the device. Applications of ColorStick technology may include children's toys, aids for the visually handicapped (e.g. blind or color blind individuals), designers, internet shoppers, gardeners, etc.

Abstract translation: 一种包括照明装置和光感测装置的装置，其可以基于在电磁光谱的至少三个区域中从着色表面弹出的反射光的大小来检查和记忆物体的离散颜色。 该设备还提供输出作为用于解密颜色的视觉和/或可听见的信号。 由装置识别的颜色范围不限于可见光谱，并且可以包括红外和紫外光。 用于存储颜色的存储装置也可以包括在装置中。 ColorStick技术的应用可能包括儿童玩具，视障人士辅助工具（如盲人或盲人），设计师，网络购物者，园丁等。

公开(公告)号：US20090000188A1

公开(公告)日：2009-01-01

申请号：US11823140

申请日：2007-06-27

Applicant: Adda Sayers ,  Dean M. Tranel ,  Travis A. Hanselman ,  Stephen D. Strachan

Inventor： Adda Sayers ,  Dean M. Tranel ,  Travis A. Hanselman ,  Stephen D. Strachan

IPC: G01N15/10 ,  G01N21/01 ,  G01N33/483 ,  G01N1/40 ,  A01G7/00

CPC classification number: G01N21/17 ,  A01G7/00 ,  G01N1/4077 ,  G01N15/06 ,  G01N15/10 ,  G01N15/1463 ,  G01N33/0098 ,  G01N2021/1765 ,  G01N2201/02 ,  G01N2201/125 ,  G06K9/00134 ,  G06Q99/00

Abstract: An apparatus and method of quantitatively obtaining a measurement of pollen of a plant. One method of counting comprises imaging the sample with the pollen well-distributed in the focal plane of the imager. Image evaluation software can identify and count objects in the image that are consistent with pollen. Total pollen count for the plant can be derived from the count of pollen of the sample, proportionality of the sample volume to the starting volume, and proportionality of area of sample imaged to total area of sample. Pollen quantification can be used for research or commercial production decisions relative to the plant or its seed.

Abstract translation: 一种定量获得植物花粉测量的装置和方法。 一种计数方法包括用分布在成像器的焦平面中的花粉成像样品。 图像评估软件可以识别和计数与花粉一致的图像中的对象。 植物的总花粉计数可以从样品的花粉计数，样品体积与起始体积的比例以及样品的面积与样品总面积的比例来得出。 花粉量化可用于相对于植物或其种子的研究或商业生产决策。