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    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS
    122.
    发明申请
    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS 有权
    用于多单细胞捕获和使用微流控的处理的方法，系统和设备

    公开(公告)号：US20130302807A1

    公开(公告)日：2013-11-14

    申请号：US13781313

    申请日：2013-02-28

    Applicant: Fluidigm Corporation

    Inventor： Brian Fowler ,  Jake Kimball ,  Myo Thu Maung ,  Andrew May ,  Michael C. Norris ,  Dominique G. Toppani ,  Marc A. Unger ,  Jing Wang ,  Jason A.A. West

    IPC: G01N1/28

    CPC classification number: G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

    Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

    Abstract translation: 描述了利用微流体的多单细胞捕获和处理的方法，系统和装置。 提供的工具和技术用于捕获，分配和/或操纵来自较大群体的细胞的单个细胞以及产生与每个单个细胞相关的遗传信息和/或反应。 可以使用不同的捕获配置来捕获单个细胞，然后在多室反应配置中处理每个单独的细胞。 一些实施方案可以提供已经从较大群体中划分的多个单个细胞的特异性靶扩增，全基因组扩增，全转录组扩增，实时PCR制备，拷贝数变异，预扩增，mRNA测序和/或单倍型 细胞。 一些实施例可以提供其他应用。 一些实施例可以被配置为用于对作为处理的一部分的各个单元或相关联的反应产物进行成像。 在某些情况下可以收获和/或进一步分析反应产物。

    METHODS AND DEVICES FOR ELECTRONIC SENSING
    123.
    发明申请
    METHODS AND DEVICES FOR ELECTRONIC SENSING 有权
    电子感应的方法和装置

    公开(公告)号：US20130302785A1

    公开(公告)日：2013-11-14

    申请号：US13867555

    申请日：2013-04-22

    Applicant: Fluidigm Corporation

    Inventor： Hany Nassef ,  Geoffrey Facer ,  Marc Unger

    IPC: G01N27/00

    CPC classification number: C03C25/68 ,  B01L3/502707 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  G01N15/1031 ,  G01N15/12 ,  G01N27/00 ,  G01N27/60 ,  G01N33/48707 ,  G01N33/48721 ,  G01N2015/1087 ,  Y10T436/2575

    Abstract: The presence of a detectable entity within a detection volume of a microfabricated elastomeric structure is sensed through a change in the electrical or magnetic environment of the detection volume. In embodiments utilizing electronic detection, an electric field is applied to the detection volume and a change in impedance, current, or combined impedance and current due to the presence of the detectable entity is measured. In embodiments utilizing magnetic detection, the magnetic properties of a magnetized detected entity alter the magnetic field of the detection volume. This changed magnetic field induces a current which can reveal the detectable entity. The change in resistance of a magnetoresistive element may also reveal the passage of a magnetized detectable entity.

    Abstract translation: 通过检测体积的电气或磁环境的变化来检测在微制造弹性体结构的检测体积内的可检测实体的存在。 在利用电子检测的实施例中，对检测体积施加电场，并且测量由于可检测实体的存在引起的阻抗，电流或组合阻抗和电流的变化。 在利用磁检测的实施例中，磁化检测实体的磁性改变了检测体积的磁场。 这种改变的磁场诱发可以揭示可检测实体的电流。 磁阻元件的电阻变化也可以揭示磁化的可检测实体的通过。

    COPY NUMBER VARIATION DETERMINATION, METHODS AND SYSTEMS
    124.
    发明申请
    COPY NUMBER VARIATION DETERMINATION, METHODS AND SYSTEMS 审中-公开

    公开(公告)号：US20130260381A1

    公开(公告)日：2013-10-03

    申请号：US13902667

    申请日：2013-05-24

    Applicant: Fluidigm Corporation

    Inventor： Ramesh Ramakrishnan

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  C12Q1/6851 ,  C12Q2545/101 ,  C12Q2527/143

    Abstract: The present invention methods and systems for determining copy number variation of a target polynucleotide in a genome of a subject including amplification based techniques. Methods can include pre-amplification of the sample followed by distribution of sample and a plurality of reaction volumes, quantitative detection of a target polynucleotide and a reference polynucleotide, and analysis so as to determine the relative copy number of the target polynucleotide sequence in the genome of the subject.

    Microfluidic chromatography
    126.
    发明申请
    Microfluidic chromatography 有权
    微流控色谱

    公开(公告)号：US20020158022A1

    公开(公告)日：2002-10-31

    申请号：US10118469

    申请日：2002-04-05

    Applicant: Fluidigm Corporation

    Inventor： Jiang Huang ,  Hou-Pu Chou ,  Marc A. Unger

    IPC: B01D015/08

    CPC classification number: B01D15/08 ,  G01N30/6065 ,  G01N30/6095 ,  G01N2030/387 ,  G01N2030/521 ,  Y10T137/86863 ,  Y10T436/2575

    Abstract: The present invention is directed to a microfluidic chromatography apparatus comprising a microfabricated fluid delivery system and a chromatography column which is in fluid communication with the fluid delivery system, and a method for producing and using the same. Preferably, the chromatography column comprises an OTLC, PCLC, or combinations thereof.

    Abstract translation: 本发明涉及包含微流体输送系统和与流体输送系统流体连通的色谱柱的微流体色谱装置及其制造和使用方法。 优选地，色谱柱包括OTLC，PCLC或其组合。

    Object oriented microfluidic design method and system
    127.
    发明申请
    Object oriented microfluidic design method and system 有权
    面向对象微流体设计方法和系统

    公开(公告)号：US20020108097A1

    公开(公告)日：2002-08-08

    申请号：US09894858

    申请日：2001-06-27

    Applicant: Fluidigm Corporation

    Inventor： Gregory Harris ,  James Montgomery ,  Michael Lee ,  Gajus Worthington

    IPC: G06F017/50

    CPC classification number: F16K99/0059 ,  B01L3/502707 ,  B01L2200/0621 ,  B01L2200/12 ,  B01L2200/14 ,  B01L2300/123 ,  B01L2400/0487 ,  B01L2400/0638 ,  B01L2400/0655 ,  F16K99/0001 ,  F16K2099/008 ,  F16K2099/0082 ,  G06F17/509 ,  G06F2217/16 ,  H01H2029/008 ,  Y10S707/99943

    Abstract: The present invention provides for the design of a microfluidic system, including a microfluidic chip or circuit, using an object oriented microfluidic computer aided design system. In an embodiment of the present invention, in a computer system having a computer memory and an object-oriented environment, a method for physically laying out a microfluidic circuit, having a plurality of microfluidic components is provided. First, a first symbol object representing a microfluidic component is placed, where the first symbol object includes a fluid channel object which represents a first fluid channel of the microfluidic component. Next, a connecting fluid channel object on a channel layer is placed, where the connecting fluid channel object represents a second fluid channel used to connect two microfluidic components of the plurality of microfluidic components. The fluid channel object is then linked to the connecting fluid channel object, where the linking represents connecting the first fluid channel to the second fluid channel.

    Abstract translation: 本发明提供了使用面向对象的微流控计算机辅助设计系统的微流体系统的设计，包括微流体芯片或电路。 在本发明的一个实施例中，在具有计算机存储器和面向对象环境的计算机系统中，提供了一种用于物理布置具有多个微流体部件的微流体电路的方法。 首先，放置表示微流体成分的第一符号对象，其中第一符号对象包括表示微流体成分的第一流体通道的流体通道对象。 接下来，放置通道层上的连接流体通道物体，其中连接流体通道物体表示用于连接多个微流体成分的两个微流体成分的第二流体通道。 然后将流体通道物体连接到连接流体通道对象，其中连接表示将第一流体通道连接到第二流体通道。

    MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS
    129.
    发明申请
    MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS 有权

    公开(公告)号：US20210324446A1

    公开(公告)日：2021-10-21

    申请号：US17166992

    申请日：2021-02-03

    Applicant: Fluidigm Corporation

    Inventor： Andrew May ,  Peilin Chen ,  Jun Wang ,  Fiona Kaper ,  Megan Anderson

    IPC: C12Q1/6806 ,  C12Q1/686 ,  B01L3/00

    Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

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