公开(公告)号：EP3368659A1

公开(公告)日：2018-09-05

申请号：EP16788673.8

申请日：2016-10-27

Applicant: Life Technologies AS

Inventor： ALMAASBAK, Hilde, Kjersti ,  AARVAK, Tanja ,  BERNSTROEM, Kerstin ,  AAMELLEM, Oystein

IPC: C12N5/0783

CPC classification number: C12N5/0636 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2501/599

Abstract: This invention relates, inter alia, to compositions of expanded T cell populations, methods for the expansion of T cell populations and methods for using such populations of cells. In some aspects, the invention relates to compositions and methods for the selective expansion of T cell subpopulations present in mixed T cell populations, as well as T cell subpopulations produced by methods for the invention.

公开(公告)号：EP3325522A1

公开(公告)日：2018-05-30

申请号：EP16739524.3

申请日：2016-07-19

Applicant: Life Technologies AS

Inventor： FONNUM, Geir ,  LUNDBERG, Per, Nils, Pontus, ,  GÖKMEN, Muhammed, Talha ,  LARSEN, Synne ,  GLOMM, Wilhelm ,  JOHNSEN, Heidi ,  SCHMID, Ruth ,  KILAAS, Lars

IPC: C08F120/54 ,  C08F2/38 ,  C08F265/10

CPC classification number: C08F265/10 ,  C08F120/54 ,  C08F265/06 ,  C08F220/56

Abstract: This invention relates to monodisperse cross-linked hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer; and (b) a crosslinker comprising at least two vinyl groups. The invention also relates to monodisperse seed particles with a Z-average diameter of from 100 nm to 1500 nm that comprise a plurality of non-crosslinked oligomers of poly N,N-dimethylacrylamide. Also provided are methods of forming the monodisperse cross-linked hydrogel polymer particles and monodisperse seed particles.

公开(公告)号：EP2643458A1

公开(公告)日：2013-10-02

申请号：EP11793720.1

申请日：2011-11-28

Applicant: Life Technologies AS

Inventor： ELLIS, Darren James ,  HOFSLOKKEN KJUS, Nini ,  FONNUM, Geir

IPC: C12N15/10

CPC classification number: C12Q1/6806 ,  C12N15/1006 ,  C12N15/1013

Abstract: A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support whilst maintaining it on the support.

公开(公告)号：EP2109669A4

公开(公告)日：2010-02-24

申请号：EP07874286

申请日：2007-11-15

Applicant: LIFE TECHNOLOGIES AS

Inventor： BREKKE OLE ,  NORDERBERG LARS ,  NEURAUTER AXL ,  SONGE PAL

IPC: C12N5/10 ,  C07K14/36 ,  C07K14/465

CPC classification number: G01N33/82 ,  C12M23/20 ,  C12M47/02 ,  C12N13/00 ,  C40B50/14 ,  G01N33/543 ,  G01N33/54326

Abstract: Methods of reversal of the binding between a biotin compound and a biotin-binding compound are disclosed. A method of reversibly releasing a biotinylated moiety from a streptavidin (or avidin) coated support is shown as an example. The strong interaction between streptavidin or avidin-biotin is made much weaker by using a combination of modified streptavidin or avidin and modified biotin like desthiobiotin or a derivative thereof like DSB-X Biotin. A protein, such as an antibody may be biotinylated with the modified biotin. When this protein is isolated by binding the modified biotin to the modified streptavidin or avidin bound to an solid surface, it may be released under very gently and very rapid conditions by addition of free biotin. In contrast to proteins obtained by the prior art release methods the protein obtained using the previously available release methods, the proteins obtained using the methods disclosed herein will maintain their native conformation. Uses of the methods in various procedures including cell detachment procedures and techniques of detection, identification, determination, purification, separation and/or isolation of target proteins or nucleic acid molecules are also described.

公开(公告)号：EP3681898A1

公开(公告)日：2020-07-22

申请号：EP18762084.4

申请日：2018-08-30

Applicant: Life Technologies AS

Inventor： JOBERT, Laure

IPC: C07H21/00 ,  C12N15/10 ,  C12Q1/6834

公开(公告)号：EP3317426B1

公开(公告)日：2020-01-15

申请号：EP16741745.0

申请日：2016-07-01

Applicant: Life Technologies Corporation ,  Life Technologies AS

Inventor： FONNUM, Geir ,  MENCHEN, Steven M. ,  LUI, Alfred ,  XIANG, Guobing ,  LARSEN, Synne

IPC: C12Q1/68 ,  G01N33/543

公开(公告)号：EP2230264B1

公开(公告)日：2019-10-09

申请号：EP10153801.5

申请日：2007-06-29

Applicant: Life Technologies AS

Inventor： Fonnum, Geir ,  Liljeroth, Lise

IPC: C08F293/00 ,  C08F287/00 ,  C08L53/00 ,  C09D153/00

公开(公告)号：EP2429706B1

公开(公告)日：2019-07-31

申请号：EP10721763.0

申请日：2010-05-11

Applicant: Life Technologies Corporation ,  Life Technologies AS

Inventor： NORDERHAUG, Lars ,  EGEBERG, Morten ,  RIVRUD, Tommy ,  NOKLEBY, Lars ,  KAHATT, Espir ,  HOGAN, Jamie ,  ZGONC, Kornelija ,  TROLSON, Norman

IPC: B01L3/00 ,  B01L3/02 ,  B01L9/00 ,  G01N35/00

公开(公告)号：EP3409777A1

公开(公告)日：2018-12-05

申请号：EP18171330.6

申请日：2011-11-28

Applicant: Life Technologies AS

Inventor： ELLIS, Darren James ,  HOFSLOKKEN KJUS, Nini ,  FONNUM, Geir

IPC: C12N15/10

CPC classification number: C12Q1/6806 ,  C12N15/1006 ,  C12N15/1013

Abstract: A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support whilst maintaining it on the support.

公开(公告)号：EP2162222B1

公开(公告)日：2018-10-31

申请号：EP08760236.3

申请日：2008-05-29

Applicant: Life Technologies AS

Inventor： ELLIS, Darren ,  HILDISCH, Tine Borgen ,  MUNDY, Jez ,  RAGNHILDSTVEIT, Erlend ,  TOFTEN, Richard Johan ,  ZGONC, Kornelija

IPC: B03C1/033 ,  B03C1/28

CPC classification number: B03C1/288 ,  B01L9/06 ,  B03C1/0332 ,  B03C2201/18 ,  B03C2201/26

Abstract: The present disclosure relates to a magnetizing portion for providing a high-gradient magnetic field in a magnetic separation device. The magnetizing portion comprises at least one magnetic assembly. The at least one magnetic assembly comprises: a plurality of magnets whereby each magnet has a north pole, south pole and a magnet axis extending between the north and south poles, and the plurality of magnets are arranged one above the other in a direction at least substantially perpendicular to the axis of each magnet in such a manner that the north and south poles of adjacent magnets are arranged alternately and a space is provide between adjacent magnets; and at least one non-magnetic spacing means arranged in the space between adjacent magnets. The present disclosure also relates to magnetic separation devices comprising at least one of the said magnetizing portions and to a method of isolating magnetically labelled particles using the magnetic separation devices.