中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

177 records (took 0.068 seconds)

    DESIGNING A SOLUBLE FULL-LENGTH HIV-1 GP41 TRIMER
    123.
    发明申请
    DESIGNING A SOLUBLE FULL-LENGTH HIV-1 GP41 TRIMER 有权
    设计一个可溶的全长HIV-1 GP41 TRIMER

    公开(公告)号：US20160207964A1

    公开(公告)日：2016-07-21

    申请号：US15080804

    申请日：2016-03-25

    Applicant: The Catholic University of America

    Inventor： Venigalla B. RAO ,  Guofen GAO

    IPC: C07K14/005 ,  C12N7/00

    CPC classification number: C07K14/005 ,  C07K2319/35 ,  C07K2319/70 ,  C07K2319/735 ,  C12N7/00 ,  C12N7/02 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12N2795/10122 ,  C12N2795/10123

    Abstract: Described herein is a soluble HIV-1 retrovirus transmembrane glycoprotein gp41 trimer (Soc-gp41M-Fd) containing a partial ectodomain and the cytoplasmic domain, that is fused to the small outer capsid (Soc) protein of bacteriophage T4 and the Foldon domain of the bacteriophage T4 fibritin (Fd). The gp41 trimer that has a prehairpin structure could be utilized to understand the mechanism of viral entry and as a candidate for development of HIV-1 vaccines, diagnostics and therapeutics. Other secondary embodiments of the gp41 proteins containing different modifications are also disclosed. According to one embodiment, the gp41 trimer is further attached to a cell penetration peptide (CPP). Methods of producing gp41 trimers are also disclosed.

    Abstract translation: 本文描述的是含有部分胞外域和细胞质结构域的可溶性HIV-1逆转录病毒跨膜糖蛋白gp41三聚体（Soc-gp41M-Fd），其与噬菌体T4的小外壳（Soc）蛋白和 噬菌体T4纤维蛋白（Fd）。 具有前贴片结构的gp41三聚体可用于了解病毒进入的机制，并且作为HIV-1疫苗，诊断和治疗学的发展的候选者。 还公开了含有不同修饰物的gp41蛋白质的其它次要实施方案。 根据一个实施方案，gp41三聚体进一步连接于细胞穿透肽（CPP）。 还公开了生产gp41三聚体的方法。

    Protein and nucleic acid delivery vehicles, components and mechanisms thereof
    124.
    发明授权
    Protein and nucleic acid delivery vehicles, components and mechanisms thereof 有权
    蛋白质和核酸递送载体，其组分和机理

    公开(公告)号：US09365867B2

    公开(公告)日：2016-06-14

    申请号：US13796263

    申请日：2013-03-12

    Applicant: THE CATHOLIC UNIVERSITY OF AMERICA

    Inventor： Venigalla B. Rao

    IPC: C12N7/00 ,  C12N15/86 ,  A61K31/7088 ,  A61K31/711 ,  C12N15/87 ,  C12N15/88 ,  C07K14/005 ,  A61K47/48 ,  A61K48/00

    CPC classification number: C12N15/86 ,  A61K31/7088 ,  A61K31/711 ,  A61K47/48776 ,  A61K47/6901 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N15/87 ,  C12N15/88 ,  C12N2795/10042 ,  C12N2795/10122 ,  C12N2795/10142 ,  C12N2795/10152

    Abstract: Complex viruses are assembled from simple protein subunits by sequential and irreversible assembly. During genome packaging in bacteriophages, a powerful molecular motor assembles at the special portal vertex of an empty prohead to initiate packaging. An aspect of the invention relates to the phage T4 packaging machine being highly promiscuous, translocating DNA into finished phage heads as well as into proheads. Single motors can force exogenous DNA into phage heads at the same rate as into proheads and phage heads undergo repeated initiations, packaging multiple DNA molecules into the same head. This shows that the phage DNA packaging machine has unusual conformational plasticity, powering DNA into an apparently passive capsid receptacle, including the highly stable virus shell, until it is full. These features allow for the design of a novel class of nanocapsid delivery vehicles.

    Abstract translation: 复杂病毒通过顺序和不可逆组装由简单蛋白质亚基组装。 在噬菌体的基因组包装过程中，一个强大的分子马达组装在一个空头颅的特殊门户顶点，开始包装。 本发明的一个方面涉及高度混杂的噬菌体T4包装机，将DNA转运到成品噬菌体头以及pro头中。 单个电机可以将外源DNA以与头孢子相同的速率强制进入噬菌体头，并且噬菌体头经历反复引发，将多个DNA分子包装在相同的头中。 这表明噬菌体DNA包装机具有不寻常的构象可塑性，将DNA提供到明显被动的衣壳容器中，包括高度稳定的病毒壳，直至其充满。 这些特征允许设计一种新型的纳米框输送车辆。

Patent Agency Ranking