Abstract:
A method and system for correcting the effect of intensity fluctuations of the transmitted light in an absorption spectroscopy system used for the detection or measurement of chemical species in a medium, whereby one or more modulation bursts are imposed onto a light beam that passes through the medium. This burst signal may be obtained by modulating the bias current of a tunable diode laser, and the modulation burst signal may be optimally at the second harmonic of the modulation frequency of a wavelength modulated beam to allow usage of the same signal path processing used for the spectroscopic detection of the measurand for a second harmonic detection system. The burst signal can be controlled using a smooth window function to minimise the effects of non-linear perturbations that are inherent in tunable diode laser wavelength modulation spectroscopy systems, of optical interference fringes (etalons) and of the residual light absorption by background chemical species or the measurand at the wavelength coinciding with the modulation burst.
Abstract:
Methods and apparatus are provided for determining weight percent of solids in a suspension using Raman spectroscopy. The methods can be utilized to acquire Raman spectral data from the suspension and to determine weight percent of solids in a process being carried out, for example, in a vessel, without the need to remove samples for analysis. The weight percent of the solids can be determined with a desired accuracy in a relatively short time, typically 10 minutes or less. The acquired Raman spectral data may be processed by chemometric software using, for example, a Partial Least Squares algorithm and data pretreatment to provide a predicted value of weight percent solids. In some embodiments, the invention is used to determine the weight percent of microparticles of a diketopiperazine in an aqueous solution.
Abstract:
An image processing apparatus includes: an interface unit configured to input an image signal from an imaging apparatus that exposes a specimen dyed with a fluorescent dye to excitation light and images fluorescence by a color imaging element; and a color correction circuit configured to retain information on a percentage of each of a component of a second color and a component of a third color with respect to a component of a first color corresponding to the excitation light in the image signal, which is determined in advance based on color filter spectral characteristics of the color imaging element, and reduce each of an amount corresponding to the percentage of the component of the second color and an amount corresponding to the percentage of the component of the third color from the input image signal.
Abstract:
Methods and apparatus are provided for determining weight percent of solids in a suspension using Raman spectroscopy. The methods can be utilized to acquire Raman spectral data from the suspension and to determine weight percent of solids in a process being carried out, for example, in a vessel, without the need to remove samples for analysis. The weight percent of the solids can be determined with a desired accuracy in a relatively short time, typically 10 minutes or less. The acquired Raman spectral data may be processed by chemometric software using, for example, a Partial Least Squares algorithm and data pretreatment to provide a predicted value of weight percent solids. In some embodiments, the invention is used to determine the weight percent of microparticles of a diketopiperazine in an aqueous solution.
Abstract:
The invention relates to an actuation and evaluation circuit for a laser diode (1) and a photodiode (3) for determining the concentration of a gas. The laser diode can generate light in the range of an absorption line of the gas. The circuit comprises a driver (10, 11, 12, 13) for generating a driving signal (17) for the laser diode (1), an assembly (8, 9) for generating a reference signal (20), and a subtractor (5) for subtracting the reference signal (20) from the signal (21) supplied by the photodiode. The invention further relates to a measuring device for determining the concentration of a gas by means of such an actuation and evaluation circuit. Finally, the invention relates to a corresponding method.
Abstract:
This disclosure relates generally to a sampling device, and more particularly, a sampling device that facilitates spectroscopic measurements with a variable path length and the necessary software controlled algorithms and methods for such a device.
Abstract:
This disclosure relates generally to a sampling device, and, more particularly, a sampling device that facilitates spectroscopic measurements with a variable path length and the necessary software controlled algorithms, and methods for using such a device.
Abstract:
The present invention reagents and methods for setting up an instruments having a multiplicity of detector channels for analyzing a multiplicity of fluorescent dyes. The present invention is particularly applicable in the field of flow cytometry.
Abstract:
A system and method for characterizing contributions to signal noise associated with charge-coupled devices adapted for use in biological analysis. Dark current contribution, readout offset contribution, photo response non-uniformity, and spurious charge contribution can be determined by the methods of the present teachings and used for signal correction by systems of the present teachings.
Abstract:
A system and method for characterizing contributions to signal noise associated with charge-coupled devices adapted for use in biological analysis. Dark current contribution, readout offset contribution, photo response non-uniformity, and spurious charge contribution can be determined by the methods of the present teachings and used for signal correction by systems of the present teachings.