公开(公告)号：EP3469009A1

公开(公告)日：2019-04-17

申请号：EP17730104.1

申请日：2017-06-07

Applicant: Life Technologies AS ,  Thermo Fisher Scientific GENEART GmbH

Inventor： ANDERSSON, Kristian ,  FONNUM, Geir ,  HEIL, Korbinian

IPC: C08F212/08 ,  C08F220/58

公开(公告)号：EP2849885B1

公开(公告)日：2015-12-30

申请号：EP13722398.8

申请日：2013-05-08

Applicant: Life Technologies AS

Inventor： NOKLEBY, Lars ,  REED, Berit ,  HILDISCH, Tine ,  NEURAUTER, Axl

IPC: B03C1/14 ,  B01L9/06 ,  G01N35/00

CPC classification number: B03C1/14 ,  B01L9/06 ,  B03C1/0332 ,  B03C1/0335 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  G01N35/0098

Abstract: This invention relates to a sample holder for isolating magnetically labelled particles from a non-magnetic medium in a plurality of samples. The holder comprises a magnetic base for applying a magnetic force to the magnetically labelled particles and a body which is mountable on the base and demountable therefrom. The body comprises an array of sample holding portions and a magnetisable member which is magnetically urged towards the magnetic base when the body is seated on the base, whereby the body is urged to remain seated on the base. The invention also relates to use of the sample holder to separate magnetic particles from a non-magnetic medium and methods of performing such a separation.

公开(公告)号：EP2643458B1

公开(公告)日：2015-08-12

申请号：EP11793720.1

申请日：2011-11-28

Applicant: Life Technologies AS

Inventor： ELLIS, Darren James ,  HOFSLOKKEN KJUS, Nini ,  FONNUM, Geir

IPC: C12N15/10

CPC classification number: C12Q1/6806 ,  C12N15/1006 ,  C12N15/1013

Abstract: A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support whilst maintaining it on the support.

公开(公告)号：EP2852617A1

公开(公告)日：2015-04-01

申请号：EP13711510.1

申请日：2013-03-06

Applicant: Life Technologies AS

Inventor： SCHJETNE, Karoline ,  AARVAK, Tanja ,  NORDERHAUG, Lars

IPC: C07K16/28

CPC classification number: C07K16/2803 ,  C07K16/00 ,  C07K16/2809 ,  C07K16/2896 ,  C07K2317/53 ,  C07K2317/55 ,  C12N5/0636

Abstract: Provided are methods and composition useful in isolating or otherwise preparing a population of target cells. In part, it relates to recombinant antibodies comprising an exogenous proteolytic cleavage site and nucleic acid molecules encoding the antibodies.

Abstract translation: 提供了可用于分离或以其它方式制备靶细胞群的方法和组合物。 其部分涉及包含外源蛋白水解切割位点和编码抗体的核酸分子的重组抗体。

公开(公告)号：EP3317426A1

公开(公告)日：2018-05-09

申请号：EP16741745.0

申请日：2016-07-01

Applicant: Life Technologies Corporation ,  Life Technologies AS

Inventor： FONNUM, Geir ,  MENCHEN, Steven M. ,  LUI, Alfred ,  XIANG, Guobing ,  LARSEN, Synne

IPC: C12Q1/68 ,  G01N33/543

CPC classification number: C12Q1/6874 ,  C08J7/14 ,  C08J2333/26 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q2525/197 ,  C12Q2527/125 ,  C12Q2565/607 ,  C12Q2565/629

Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.

公开(公告)号：EP2993232A1

公开(公告)日：2016-03-09

申请号：EP15179788.3

申请日：2011-11-28

Applicant: Life Technologies AS

Inventor： ELLIS, Darren James ,  HOFSLOKKEN KJUS, Nini ,  FONNUM, Geir

IPC: C12N15/10

CPC classification number: C12Q1/6806 ,  C12N15/1006 ,  C12N15/1013

Abstract: A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support whilst maintaining it on the support.

Abstract translation: 一种用于处理核酸的方法，其中所述核酸暴露于水性介质，所述水性介质包含足够比例的多元醇，用于至少一部分所述核酸进入或保留在超溶质相中。 因此，多元醇可用于将溶液中的核酸与固体支持物结合，或将固体支持物上的核酸洗涤，同时将其保持在载体上。