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公开(公告)号：US10436698B2
公开(公告)日：2019-10-08
申请号：US16230570
申请日：2018-12-21
Applicant: Fluidigm Corporation
Inventor： Dmitry R. Bandura , Vladimir I. Baranov , Scott D. Tanner
Abstract: An analytical instrument has a sample introduction system for generating a stream of particles from a sample. An ionization system atomizes and ionizes particles in the stream as they are received. The instrument has an ion pretreatment system and a mass analyzer. The ion pretreatment system is adapted to transport ions generated by the ionization system to the mass analyzer. The mass analyzer is adapted measure the amount of at least one element in individual particles from the stream by performing mass analysis on the ions from the atomized particles. The instrument can be adapted to measure the amount of many different tags, for example at least five different tags, at the same time to facilitate multi-parametric analysis of cells and other particles.
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公开(公告)号：US20190292581A1
公开(公告)日：2019-09-26
申请号：US16373507
申请日：2019-04-02
Applicant: Fluidigm Corporation
Inventor： Camila Egidio , Ramesh Ramakrishnan , David Ruff
IPC: C12Q1/6804 , C12Q1/6848 , C12Q1/6851
Abstract: Methods and reagents for detection and analysis of nucleic acids and proteins using proximity extension assays.
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公开(公告)号：US20190249238A1
公开(公告)日：2019-08-15
申请号：US16229786
申请日：2018-12-21
Applicant: Fluidigm Corporation
Inventor： Jason A.A. West , Brian Fowler , Tze-Howe Charn , Christian F. Johnson , Marc A. Unger
IPC: C12Q1/6855 , C12N15/10
CPC classification number: C12Q1/6855 , C12N15/1065 , C12Q1/6806 , C12Q2565/501 , C12Q2521/301 , C12Q2521/501 , C12Q2525/155 , C12Q2537/162 , C12Q2565/543 , C12Q2535/122
Abstract: This disclosure provides a method of forming tagged nucleic acid sequences. A target polynucleotide is immobilized on a solid support; a recognition-oligonucleotide is hybridized thereto; the recognition-oligonucleotide-target polynucleotide hybrid is cleaved; and an adapter nucleic acid is ligated to the cleaved target polynucleotide, thereby forming a tagged nucleic acid sequence. Also provided is a method of forming a tagged single stranded cDNA; a method of forming a plurality of tagged heterogeneous nucleic acid sequences; a library of recognition-oligonucleotides; and methods for amplifying a cDNA sequence immobilized on a solid support. These methods and products can be used alone or in combination for integrated single cell sequencing, and can be adapted for use in a microfluidic apparatus or device.
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公开(公告)号：US20190217302A1
公开(公告)日：2019-07-18
申请号：US16245537
申请日：2019-01-11
Applicant: Fluidigm Corporation
Inventor： Jake Kimball , Brandon Ripley , Gang Sun , Dominique Toppani , Myo Thu Maung
CPC classification number: B01L7/52 , B01L3/5027 , B01L2200/025 , B01L2200/147 , B01L2300/0819 , B01L2300/0893 , B01L2300/1805 , B01L2300/1822 , C12P19/34 , C12Q3/00 , G01N21/6408 , G01N21/6428 , G01N21/6456 , G01N21/76
Abstract: A thermal cycler for a microfluidic device includes a controller operable to provide a series of electrical signals, a heat sink, and a heating element in thermal communication with the heat sink and operable to receive the series of electrical signals from the controller. The thermal cycler also includes a thermal chuck in thermal communication with the heating element. The thermal chuck comprises a heating surface operable to make thermal contact with the microfluidic device. The heating surface is characterized by a temperature ramp rate between 2.5 degrees Celsius per second and 5.5 degrees Celsius per second and a temperature difference between a first portion of the heating surface supporting a first portion of the microfluidic device and a second portion of the heating surface supporting a second portion of the microfluidic device is less than 0.25° C.
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公开(公告)号：US20190195771A1
公开(公告)日：2019-06-27
申请号：US16230570
申请日：2018-12-21
Applicant: Fluidigm Corporation
Inventor： Dmitry R. Bandura , Vladimir I. Baranov , Scott D. Tanner
CPC classification number: G01N15/1404 , G01N2015/0065 , G01N2458/15 , H01J49/004 , H01J49/04 , H01J49/0431
Abstract: An analytical instrument has a sample introduction system for generating a stream of particles from a sample. An ionization system atomizes and ionizes particles in the stream as they are received. The instrument has an ion pretreatment system and a mass analyzer. The ion pretreatment system is adapted to transport ions generated by the ionization system to the mass analyzer. The mass analyzer is adapted measure the amount of at least one element in individual particles from the stream by performing mass analysis on the ions from the atomized particles. The instrument can be adapted to measure the amount of many different tags, for example at least five different tags, at the same time to facilitate multi-parametric analysis of cells and other particles.
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Patent Agency Ranking
公开(公告)号：US20190153511A1
公开(公告)日：2019-05-23
申请号：US16134855
申请日：2018-09-18
Applicant: Fluidigm Corporation
Inventor： Marc A. Unger , Geoffrey Richard Facer , Barry Clerkson , Christopher G. Cesar , Neil Switz
CPC classification number: C12Q1/686 , B01J2219/00576 , B01J2219/00704 , B01L3/5027 , B01L7/52 , G01N21/64 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/75 , G01N21/8483 , G01N27/44721 , G01N2021/6421 , G01N2201/061 , G02B21/16 , G02B21/36
Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.
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公开(公告)号：US10227624B2
公开(公告)日：2019-03-12
申请号：US14800548
申请日：2015-07-15
Applicant: Fluidigm Corporation
Inventor： Peilin Chen , Jing Wang , Andrew May
IPC: C12Q1/68 , C12P19/34 , C12Q1/6844 , C12Q1/6848 , C12Q1/683
Abstract: The present invention provides reagents, kits, and methods for single-cell whole genome amplification using Phi 29 DNA polymerase.
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公开(公告)号：US10214773B2
公开(公告)日：2019-02-26
申请号：US15724190
申请日：2017-10-03
Applicant: Fluidigm Corporation
Inventor： Robert C. Jones
IPC: C12Q1/68 , C12Q1/686 , C12Q1/682 , G01N33/532 , G01N33/53
Abstract: Methods, reagents, and kits for detection and analysis of nucleic acids are provided. The methods can be used in conjunction with a proximity extension assay for protein detection to provide a multiplex assay to detect both nucleic acids (e.g., RNA) and proteins.
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公开(公告)号：US10180386B2
公开(公告)日：2019-01-15
申请号：US15888871
申请日：2018-02-05
Applicant: Fluidigm Corporation
Inventor： Dmitry R. Bandura , Vladimir I. Baranov , Scott D. Tanner
Abstract: A method for cellular analysis of cellular particles tagged with elemental tags, such as lanthanide-based elemental tags. Particles or element tags associated with particles can be vaporized, atomized, and ionized, such as with an inductively coupled plasma device or a glow discharge device. The vaporized, atomized, and ionized particles or element tags can be analyzed using mass spectrometry, such as using a time of flight mass spectrometer or a magnetic sector mass spectrometer. The amount of at least one element in individual particles can be measured through mass analysis. The amount of many different tags, for example at least five different tags, can be measured at the same time to facilitate multi-parametric analysis of cells and other particles. The vaporized, atomized, and ionized particles or element tags can be pretreated in an ion pretreatment system to filter out low mass ions, such as using a high-pass mass filter or a bandpass mass filter, to allow the elemental tags to pass therethrough.
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公开(公告)号：US10131934B2
公开(公告)日：2018-11-20
申请号：US15805720
申请日：2017-11-07
Applicant: Fluidigm Corporation
Inventor： Marc Unger , Ian D. Manger , Michael Lucero , Yong Yi , Emily Miyashita-Lin , Anja Wienecke , Geoffrey Facer
Abstract: A method for carrying out nucleic acid amplification reactions using a microfluidic device is described. Amplification primers and other amplification reagents are deposited at a plurality of reaction sites in the device, a sample solution containing amplifiable polynucleotides is introduced into the reaction sites, and amplification is carried out.
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