公开(公告)号：US08034629B2

公开(公告)日：2011-10-11

申请号：US12552268

申请日：2009-09-01

Applicant: Stephen C. Chapin ,  Patrick Seamus Doyle ,  Daniel Colin Pregibon

Inventor： Stephen C. Chapin ,  Patrick Seamus Doyle ,  Daniel Colin Pregibon

IPC: G01N1/10

CPC classification number: B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/123 ,  B01L2400/084 ,  Y10T428/2982 ,  Y10T436/117497 ,  Y10T436/2575

Abstract: Techniques are provided for high precision scanning of hydrogel microparticles. The high precision is achieved by one or more modifications to the microparticle composition, or microfluidics apparatus that align the microparticles in a detection channel, or method of preparing a sample for introduction into the apparatus, or some combination. An apparatus comprises a body structure having formed therein a central channel and multiple focusing channels in fluid communication with the central channel through multiple junctions. A width of the central channel is smaller in a portion downstream of each junction. A particle comprises a hydrogel matrix and a probe molecule. The particle has an aspect ratio greater than about three. A method includes loading into a sample fluid inlet a mixture, wherein a number of particles lies within a range from about 15 to about 20 particles/μl.

Abstract translation: 提供技术用于水凝胶微粒的高精度扫描。 通过对微粒组合物的一个或多个修改或者将微粒对准检测通道的微流体装置或者制备用于引入到装置中的样品的方法或一些组合来实现高精度。 一种装置包括其中形成有中心通道的主体结构和通过多个连接部与中心通道流体连通的多个聚焦通道。 中央通道的宽度在每个连接点的下游部分较小。 颗粒包含水凝胶基质和探针分子。 颗粒的纵横比大于约3。 一种方法包括将样品流体入口装载到混合物中，其中许多颗粒位于约15至约20个颗粒/μl的范围内。