公开(公告)号：US20030024012A1

公开(公告)日：2003-01-30

申请号：US10133205

申请日：2002-04-26

Applicant: ID-Lelystad, Instituut voor Dierhouderijen Diergezondheid B.V.

Inventor： Latifa Abdennebi-Najar ,  Hendrikus Antonius C. Bakker ,  Hendrik Jan Bosch ,  Dietmar Dirnberger ,  Jean-Jacques Remy ,  Herta Steinkeller ,  Dirk Franciscus, M. van de Wiel

IPC: A01H005/00

CPC classification number: C12N9/1051 ,  C12N15/8257 ,  C12N15/8258

Abstract: The invention relates to the production of gonadotrophins and corresponding receptors in transgenic plants. The invention provides a method to produce a gonadotrophin or its corresponding receptor in a transgenic plant with modified glycosylation machinery, in order to allow for mammalian type of glycosidic side chains of gonadotrophin and its corresponding receptor.

Abstract translation: 本发明涉及转基因植物中促性腺激素和相应受体的产生。 本发明提供了一种在具有修饰的糖基化机制的转基因植物中产生促性腺激素或其相应受体的方法，以便允许哺乳动物类型的促性腺激素及其相应受体的糖苷侧链。

公开(公告)号：WO2002096940A2

公开(公告)日：2002-12-05

申请号：PCT/NL2002/000353

申请日：2002-05-31

Applicant: ID-LELYSTAD, INSTITUT VOOR DIERHOUDERIJ EN DIERGEZONDHEID B.V. ,  BOOT, Hendrik, Johannis

Inventor： BOOT, Hendrik, Johannis

IPC: C07K14/08

CPC classification number: A61K39/12 ,  A61K2039/5254 ,  C07K14/005 ,  C12N7/00 ,  C12N2720/10022 ,  C12N2720/10034 ,  C12N2720/10051 ,  C12N2720/10061 ,  C12N2720/10064

Abstract: The invention relate to the field of prevention or treatment Infectious Bursal Disease virus infections. The invention provides a method for the generation of attenuated vvIBDV and vaccines based thereon through alteration of the primary sequence of an essentially untranslated region of the viral nucleic acid.

Abstract translation: 本发明涉及预防或治疗领域传染性法氏囊病病毒感染。 本发明提供了通过改变病毒核酸的基本上非翻译区的一级序列来产生减毒的vvIBDV和疫苗的方法。

公开(公告)号：WO2002095040A1

公开(公告)日：2002-11-28

申请号：PCT/NL2002/000314

申请日：2002-05-16

Applicant: ID-LELYSTAD, INSTITUUT VOOR DIERHOUDERIJ EN DIERGEZONDHEID B.V. ,  VERHEIJE, Monique, Helene

Inventor： VERHEIJE, Monique, Helene

IPC: C12N15/86

CPC classification number: C07K14/005 ,  A61K39/00 ,  A61K2039/51 ,  A61K2039/53 ,  C12N15/85 ,  C12N2770/10022

Abstract: The invention relates to recombinant Arterivirus replicons. The invention provides the insight that an Arterivirus replicon having at least some of its original arteriviral nucleic acid encoding ORF-7 deleted, as provided herein, can still be capable of in vivo RNA replication, even when further comprising nucleic acid derived from at least one heterologous micro-organism, thereby also providing viable Arteriviruses with deletions proximal to the 3'end of the genome.

Abstract translation: 本发明涉及重组动脉病毒复制子。 本发明提供的见解是，如本文提供的，具有编码ORF-7的至少一些其原始动脉病毒核酸的动脉病毒复制子仍然能够进行体内RNA复制，即使进一步包含 来自至少一种异源微生物的核酸，从而也提供具有基因组3'末端附近缺失的可行的动脉病毒。

公开(公告)号：WO2002061070A3

公开(公告)日：2002-08-08

申请号：PCT/NL2002/000073

申请日：2002-01-31

Applicant: ID-LELYSTAD, INSTITUUT VOOR DIERHOUDERIJ EN DIERGEZONDHEID B.V. ,  SMITH, Hilda, Elizabeth

Inventor： SMITH, Hilda, Elizabeth

IPC: C07K14/315

Abstract: The invention relates to the field of diagnosis of and vaccination against Streptococcal infections and to the detection of virulence markers of Streptococci. The invention provides a method for modulating virulence of a Streptococcus comprising modifying a genomic fragment of said Streptococcus wherein said genomic fragment comprises at least a functional part of a fragment identifiable by hybridisation in Streptococcus suis to a nucleic acid or fragment thereof.

公开(公告)号：WO2004039400A3

公开(公告)日：2004-11-11

申请号：PCT/NL0300762

申请日：2003-11-03

Applicant: ID LELYSTAD INST DIERHOUDERIJ

Inventor： RIJSEWIJK FRANCISCUS ANTONIUS

IPC: A61K39/205 ,  A61K39/245 ,  C07K14/03 ,  C07K14/145 ,  C12N15/82 ,  C07K14/005

CPC classification number: C07K14/005 ,  A61K39/12 ,  A61K39/205 ,  A61K39/245 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/70 ,  C12N2710/16722 ,  C12N2710/16734 ,  C12N2710/16743 ,  C12N2760/20122 ,  C12N2760/20134

Abstract: The invention relates to the field of encephalitis in ruminants. The invention provides a mutant of bovine herpesvirus 5 (BHV5) provided with a functional deletion in each of at least two genes related to the clinical manifestation of neurovirulence upon infection of a host with BHV5. Furthermore, the invention provides a vaccine comprising such a mutant. The invention further provides a method for distinguishing a ruminant infected with a wild-type BHV5 strain or vaccinated against BHV5 from within a population of ruminants vaccinated with a vaccine according to the invention comprising testing a ruminant for the presence or absence of an antibody directed against the glycoprotein I (gI) gene or the glycoprotein E (gE) gene or both.

Abstract translation: 本发明涉及反刍动物中的脑炎领域。 本发明提供了一种牛疱疹病毒5突变体（BHV5），其在用BHV5感染宿主后与至少两种与神经毒力的临床表现有关的基因中的每一个中都具有功能缺失。 此外，本发明提供了包含这种突变体的疫苗。 本发明进一步提供了一种区分用本发明疫苗接种的反刍动物种群内感染野生型BHV5株或针对BHV5接种的反刍动物的方法，包括检测反刍动物是否存在针对抗体的抗体 糖蛋白I（gI）基因或糖蛋白E（gE）基因或两者。

公开(公告)号：WO2003058248A2

公开(公告)日：2003-07-17

申请号：PCT/NL2003/000020

申请日：2003-01-13

Applicant: ID-LELYSTAD, INSTITUUT VOOR DIERHOUDERIJ EN DIERGEZONDHEID B.V. ,  WILLEMSEN, Petrus, Theodorus, Johannes ,  WESTERVEEN, Sjoukje, Fetsje ,  BAKKER, Douwe ,  VAN ZIJDERVELD, Fred, Govert ,  THOLE, Jelle, Egbertus, Rudolfus

Inventor： WILLEMSEN, Petrus, Theodorus, Johannes ,  WESTERVEEN, Sjoukje, Fetsje ,  BAKKER, Douwe ,  VAN ZIJDERVELD, Fred, Govert ,  THOLE, Jelle, Egbertus, Rudolfus

IPC: G01N33/569

CPC classification number: C07K14/35 ,  A61K39/00 ,  A61K2039/505 ,  A61K2039/51 ,  A61K2039/53

Abstract: The present invention relates to nucleic acid sequences encoding Mycobacterium avium subspecies paratuberculosis proteins, to parts of such nucleic acid sequences that encode an immunogenic fragment of such proteins, to DNA fragments, recombinant DNA molecules, live recombinant carriers and host cells comprising such nucleic acid sequences or such parts thereof. The invention also relates to Mycobacterium avium subspecies paratuberculosis proteins and immunogenic parts thereof encoded by such sequences. Furthermore, the present invention relates to vaccines comprising such nucleic acid sequences and parts thereof, DNA fragments, recombinant DNA molecules, live recombinant carriers and host cells comprising such nucleic acid sequences or such parts thereof, proteins or immunogenic parts thereof and antibodies against such proteins or immunogenic parts thereof. Also, the invention relates to the use of said proteins in vaccines and for the manufacture of vaccines. Moreover, the invention relates to the use of said nucleic acid sequences, proteins or antibodies for diagnostic or vaccination purposes. Also, the invention relates to methods for the preparation of such vaccines. Finally the invention relates to diagnostic kits comprising such nucleic acids, proteins or antibodies against such proteins.

Abstract translation: 本发明涉及将编码鸟分枝杆菌亚种副结核分枝杆菌蛋白的核酸序列与编码这种蛋白质的免疫原性片段的这些核酸序列的部分部分涉及DNA片段，重组DNA分子，活重组载体和包含该核酸序列的宿主细胞 或其部分。 本发明还涉及由这种序列编码的鸟分枝杆菌亚型副结核杆菌蛋白及其免疫原性部分。 此外，本发明涉及包含此类核酸序列及其部分的DNA疫苗，DNA片段，重组DNA分子，活重组载体和包含此类核酸序列或其部分的宿主细胞，蛋白质或其免疫原性部分和针对此类蛋白质的抗体 或其免疫原性部分。 此外，本发明涉及所述蛋白质在疫苗中的用途以及制造疫苗。 此外，本发明涉及用于诊断或接种目的的所述核酸序列，蛋白质或抗体的用途。 此外，本发明涉及制备这种疫苗的方法。 最后，本发明涉及包含这种蛋白质的核酸，蛋白质或抗体的诊断试剂盒。

公开(公告)号：WO2002066686A1

公开(公告)日：2002-08-29

申请号：PCT/NL2002/000109

申请日：2002-02-19

Applicant: ID-LELYSTAD, INSTITUUT VOOR DIERHOUDERIJ EN DIERGEZONDHEID B.V. ,  EGBERINK, Hermanus, Fransiscus ,  SIMONS, Fermin, Adrianus

Inventor： EGBERINK, Hermanus, Fransiscus ,  SIMONS, Fermin, Adrianus

IPC: C12Q1/70

CPC classification number: C12Q1/701

Abstract: The invention relates to a method to differentiate feline infectious peritonitis viruses (FIPV) from feline enteric coronaviruses (FECV). The invention provides a method allowing for antemortem diagnosis for distinguishing a feline infectious peritonitis virus (FIPV) infection from a feline enteric coronavirus (FECV) infection comprising detecting coronavirus in non-enteric cells in a sample obtained from a cat suspected of being infected with FIPV and/or FECV. Provided are methods for the detection of a replicating FIPV in order to distinguish a FIPV coronavirus infection from a FECV infection, including diagnostic kits and a cat or cat population diagnosed with such kits.

Abstract translation: 本发明涉及将猫感染性腹膜炎病毒（FIPV）与猫肠道冠状病毒（FECV）区分开的方法。 本发明提供了一种允许用于区分来自猫肠道冠状病毒（FECV）感染的猫感染性腹膜炎病毒（FIPV）感染的方法的方法，其包括从怀疑感染FIPV的猫获得的样品中检测非肠道细胞中的冠状病毒 和/或FECV。 提供了用于检测复制FIPV的方法，以区分FIPV冠状病毒感染与FECV感染，包括诊断试剂盒和诊断患有这些试剂盒的猫或猫群体。

公开(公告)号：WO2002061070A2

公开(公告)日：2002-08-08

申请号：PCT/NL2002/000073

申请日：2002-01-31

Applicant: ID-LELYSTAD, INSTITUUT VOOR DIERHOUDERIJ EN DIERGEZONDHEID B.V. ,  SMITH, Hilda, Elizabeth

Inventor： SMITH, Hilda, Elizabeth

IPC: C12N15/00

CPC classification number: C07K14/315 ,  A61K39/00

Abstract: The invention relates to the field of diagnosis of and vaccination against Streptococcal infections and to the detection of virulence markers of Streptococci. The invention provides a method for modulating virulence of a Streptococcus comprising modifying a genomic fragment of said Streptococcus wherein said genomic fragment comprises at least a functional part of a fragment identifiable by hybridisation in Streptococcus suis to a nucleic acid or fragment thereof.

Abstract translation: 本发明涉及链球菌感染的诊断和接种领域以及链球菌的毒力标记物的检测领域。 本发明提供了一种调节链球菌的毒力的方法，其包括修饰所述链球菌的基因组片段，其中所述基因组片段至少包含通过在猪链球菌中与核酸或其片段杂交可鉴定的片段的功能部分。

公开(公告)号：WO0200882A3

公开(公告)日：2002-06-27

申请号：PCT/NL0100484

申请日：2001-06-28

Applicant: ID LELYSTAD INST DIERHOUDERIJ ,  LANGEDIJK JOHANNES PETRUS MARI

Inventor： LANGEDIJK JOHANNES PETRUS MARI

IPC: C12N15/09 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61P31/04 ,  C07K14/135 ,  C07K14/18 ,  C07K14/185 ,  C07K16/10 ,  C12N15/40 ,  G01N33/569

CPC classification number: C07K14/005 ,  A61K39/00 ,  C12N2770/24322

Abstract: The invention relates to peptides derived from or similar to the E protein of pestiviruses for type -specific diagnosis of infection, for eliciting antibiotic activity and for transport of substances into a cell. For among others these purposes the invention provides an isolated, synthetic or recombinant protein module or functional equivalent thereof comprising an amino acid sequence that is at least 75 % identical to an amino acid sequence of a peptide located from at about amino acid position 194 to 220 in a pestiviral E protein.

Abstract translation: 本发明涉及衍生自或类似于瘟病毒E蛋白的肽，用于感染的特异性诊断，用于引发抗生素活性和将物质运输到细胞中。 对于其它的这些目的，本发明提供了分离的，合成的或重组的蛋白质模块或其功能等同物，其包含与位于大约氨基酸位置194至220的肽的氨基酸序列至少75％相同的氨基酸序列 在瘟病毒E蛋白中。

公开(公告)号：WO2006006853A3

公开(公告)日：2006-03-09

申请号：PCT/NL2005000494

申请日：2005-07-08

Applicant: ID LELYSTAD INST DIERHOUDERIJ ,  NIEWOLD THEODOOR ABRAM ,  REBEL JOHANNA MARINA JACOBA ,  SMITS MARINUS ADRIANUS

Inventor： NIEWOLD THEODOOR ABRAM ,  REBEL JOHANNA MARINA JACOBA ,  SMITS MARINUS ADRIANUS

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q2600/124 ,  C12Q2600/158

Abstract: The invention provides a set of genes or gene sequences comprising at least 2 genes and the use of said set of genes or gene sequences for the determination of intestinal health, and/or disease of an animal or a human. The invention further provides methods to detect the presence or absence of an intestinal disease in an animal or a human comprising measuring, in a sample of said animal or human, expression levels of a set of genes or gene sequences according to the invention, or a gene specific fragment of said genes and comparing said expression levels with a reference value such as the expression levels of said set of genes in a sample of intestinal tissue of an healthy animal or human.

Abstract translation: 本发明提供了一组包含至少2个基因的基因或基因序列以及所述组基因或基因序列用于测定动物或人的肠健康和/或疾病的用途。 本发明进一步提供了检测动物或人中肠道疾病的存在或不存在的方法，包括在所述动物或人的样品中测量根据本发明的一组基因或基因序列的表达水平或 基因特异性片段，并将所述表达水平与参考值比较，例如健康动物或人的肠组织样品中所述基因组的表达水平。