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    Method for direct amplification from crude nucleic acid sample
    14.
    发明专利
    Method for direct amplification from crude nucleic acid sample 审中-公开
    从原核细胞样品直接放大的方法

    公开(公告)号：JP2014166189A

    公开(公告)日：2014-09-11

    申请号：JP2014122337

    申请日：2014-06-13

    Applicant: Life Technologies Corp ,  ライフ テクノロジーズ コーポレーション

    Inventor： CHANG CHIEN-WEI ,  DENNIS Y WANG ,  LORI K HENNESSY

    IPC: C12N15/09 ,  C12Q1/68

    CPC classification number: C12Q1/6888 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q2527/125

    Abstract: PROBLEM TO BE SOLVED: To provide a method for direct amplification from crude nucleic acid samples.SOLUTION: The present teachings relate to improved methods, kits, and reaction mixtures for amplifying nucleic acids. In some embodiments a novel direct buffer formulation is provided which allows for the direct amplification of the nucleic acids in a crude sample with minimal sample purification.The present teachings provide a method of performing a polymerase chain reaction (PCR) comprising; providing a crude sample comprising deoxyribonucleic acid; optionally incubating the crude sample with NaOH at 5 mM to 25 mM; mixing the crude sample with a direct buffer to form a nucleic acid containing solution; and performing a PCR on the deoxyribonucleic acid.

    Abstract translation: 要解决的问题：提供从粗核酸样品直接扩增的方法。解决方案：本教导涉及用于扩增核酸的改进的方法，试剂盒和反应混合物。 在一些实施方案中，提供了一种新的直接缓冲液制剂，其允许以最少的样品纯化直接扩增粗样品中的核酸。本教导提供了进行聚合酶链式反应（PCR）的方法，其包括： 提供包含脱氧核糖核酸的粗样品; 任选地将粗样品与5mM至25mM的NaOH温育; 将粗样品与直接缓冲液混合以形成含有核酸的溶液; 并对脱氧核糖核酸进行PCR。

    Field effect transistor device for ultra-fast nucleic acid sequencing
    15.
    发明专利
    Field effect transistor device for ultra-fast nucleic acid sequencing 审中-公开
    用于超快速核酸序列的场效应晶体管器件

    公开(公告)号：JP2011177180A

    公开(公告)日：2011-09-15

    申请号：JP2011082517

    申请日：2011-04-04

    Applicant: Life Technologies Corp ,  ライフ テクノロジーズ コーポレーション

    Inventor： SAUER JON ,  VAN ZEGHBROECK BART

    IPC: C12M1/00 ,  G01N33/483 ,  C12N15/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N15/12 ,  G01N27/00 ,  G01N27/414 ,  G01N33/50 ,  H01L29/66

    CPC classification number: G01N33/48721 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0645 ,  B01L2400/0436 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N27/414 ,  H01L2924/0002 ,  C12Q2565/501 ,  C12Q2565/607 ,  H01L2924/00

    Abstract: PROBLEM TO BE SOLVED: To provide a system and method for accurately and effectively identifying bases of DNA or RNA. SOLUTION: A field effect transistor (FET), nucleic acid sequencing device (102) comprises source (106) and drain (104) regions, and gate oxide (136). Potential is applied to the source and drain by voltage source (112) through leads (116) connected to the source/drain metal contacts (123, 142). As nucleic acid strand (111) passes through opening (118) which serves as the gate electrode region, the charge representative of a nucleic acid base (adenine, thymine, guanine, or cytosine) modifies the current flowing between source (106) and drain (104) via channel (119) by modifying the electric field therebetween and is measured by ammeter (114). COPYRIGHT: (C)2011,JPO&INPIT

    Abstract translation: 要解决的问题：提供准确有效地鉴定DNA或RNA的碱基的系统和方法。 解决方案：场效应晶体管（FET），核酸测序装置（102）包括源极（106）和漏极（104）区域以及栅极氧化物（136）。 电压通过电压源（112）通过连接到源极/漏极金属触点（123,142）的引线（116）施加到源极和漏极。 当核酸链（111）通过用作栅电极区的开口（118）时，代表核酸碱基（腺嘌呤，胸腺嘧啶，鸟嘌呤或胞嘧啶）的电荷修饰在源（106）和漏极（106）之间流动的电流 （114）通过通道（119）修改其间的电场，并通过电流表（114）测量。 版权所有（C）2011，JPO＆INPIT

    Composition and method for enhancing synthesis of nucleic acid molecule
    17.
    发明专利
    Composition and method for enhancing synthesis of nucleic acid molecule 审中-公开
    用于增强核酸分子合成的组合物和方法

    公开(公告)号：JP2011041573A

    公开(公告)日：2011-03-03

    申请号：JP2010238335

    申请日：2010-10-25

    Applicant: Life Technologies Corp ,  ライフ テクノロジーズ コーポレーション

    Inventor： LI WU-BO ,  JESSEE JOEL A ,  SCHUSTER DAVID ,  XIA JIULIN ,  GEBEYEHU GULILAT

    IPC: C12N15/09 ,  C07H21/00 ,  C12P19/34 ,  C12Q1/68

    CPC classification number: C12P19/34 ,  C07H21/00 ,  C12Q1/6848 ,  C12Q2527/125

    Abstract: PROBLEM TO BE SOLVED: To provide compositions and methods for enhancing synthesis of nucleic acid molecules, particularly GC-rich nucleic acid molecules.
    SOLUTION: The compositions comprise one or more nitrogen-containing organic compounds selected from the group consisting of specific constitutional formulae (or salts or derivatives thereof), preferably 4-methylmorpholine N-oxide or betaine (carboxymethyltrimethylammonium), and further comprises one or more compounds selected from the group consisting of proline and an N-alkylimidazole compound, and more preferably proline, 1-methylimidazole or 4-methylimidazole. The invention further relates to methods for high-fidelity synthesis of nucleic acid molecules, including via amplification, reverse transcription, and sequencing. The invention also relates to nucleic acid molecules synthesized by these methods or derivatives thereof, and to vectors and host cells comprising such nucleic acid molecules or derivatives. The invention also relates to kits for synthesizing, amplifying, reverse transcribing, or sequencing nucleic acid molecules comprising one or more of the compositions.
    COPYRIGHT: (C)2011,JPO&INPIT

    Abstract translation: 待解决的问题：提供用于增强核酸分子，特别是富含GC的核酸分子的合成的组合物和方法。 组合物包含一种或多种选自特定结构式（或其盐或衍生物），优选4-甲基吗啉N-氧化物或甜菜碱（羧甲基三甲基铵）的含氮有机化合物，并且还包含一种 或更多选自脯氨酸和N-烷基咪唑化合物的化合物，更优选脯氨酸，1-甲基咪唑或4-甲基咪唑。 本发明还涉及核酸分子的高保真合成方法，包括经由扩增，逆转录和测序。 本发明还涉及通过这些方法或其衍生物合成的核酸分子以及包含该核酸分子或衍生物的载体和宿主细胞。 本发明还涉及用于合成，扩增，逆转录或测序包含一种或多种组合物的核酸分子的试剂盒。 版权所有（C）2011，JPO＆INPIT

    Dry powder cell, cell culture reagent and method of production thereof
    20.
    发明专利
    Dry powder cell, cell culture reagent and method of production thereof 审中-公开
    干粉细胞，细胞培养物试剂及其生产方法

    公开(公告)号：JP2010233580A

    公开(公告)日：2010-10-21

    申请号：JP2010141177

    申请日：2010-06-22

    Applicant: Life Technologies Corp ,  ライフ テクノロジーズ コーポレーション

    Inventor： FIKE RICHARD ,  WHITFORD WILLIAM ,  BIDDLE WILLIAM

    IPC: C12N1/00 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N5/00 ,  C12N5/02 ,  C12N5/07

    CPC classification number: C12N5/0018 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N5/0025

    Abstract: PROBLEM TO BE SOLVED: To provide nutritive medium, medium supplement suitable for culture of bacterial cells, yeast cells, plant cells and animal cells, and to provide methods of production thereof. SOLUTION: There are provided powder nutritive medium, particularly cell culture medium supplement including powdered sera, and method for production of medium thereof, and medium supplement. There are also provided kits and methods for cultivation of prokaryotic and eukaryotic cells by using these powdered nutritive medium, medium supplement, a method for sterilization accomplished by gamma irradiation, and a method for producing a medium including a step for spray-drying a cell suspension. There are provided methods for production of sterilized powdered medium, medium supplement (including powdered sera, powdered transferrin, powdered insulin, powdered growth factors, etc.), media subgroup and buffer formulations, and produced cells, media, media supplement, media subgroup and powdered buffer formulations. COPYRIGHT: (C)2011,JPO&INPIT

    Abstract translation: 要解决的问题：提供适合细菌细胞培养的培养基，酵母细胞，植物细胞和动物细胞的营养培养基，并提供其生产方法。 提供粉末营养培养基，特别是细胞培养基补充剂，包括粉末状血清，以及其培养基的生产方法和中等补充剂。 还提供了通过使用这些粉状营养培养基，培养基补充剂，通过γ辐射完成的灭菌方法来培养原核和真核细胞的试剂盒和方法，以及用于产生包括用于喷雾干燥细胞悬浮液的步骤的培养基的方法 。 提供了灭菌粉末培养基，培养基补充剂（包括粉末血清，粉末转铁蛋白，胰岛素粉末，粉末生长因子等），培养基亚组和缓冲液配方的生产方法，生产的细胞，培养基，培养基，培养基和培养基 粉状缓冲剂配方。 版权所有（C）2011，JPO＆INPIT

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