公开(公告)号：WO2007142692A2

公开(公告)日：2007-12-13

申请号：PCT/US2006/060867

申请日：2006-11-14

Applicant: THE GOVERNMENT OF THE USA, AS REPRESENTED BY THE SECRETARY OF THE NAVY ,  LIGLER, Frances, S. ,  STENGER, David, A. ,  ERICKSON, Jeff ,  ARCHER, Marie

Inventor： LIGLER, Frances, S. ,  STENGER, David, A. ,  ERICKSON, Jeff ,  ARCHER, Marie

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: B01L3/5027 ,  B01L7/52 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0631 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/1827 ,  B01L2400/0421 ,  B01L2400/0439 ,  B01L2400/0487 ,  C12Q2565/629 ,  G01N2035/00158

Abstract: An apparatus having within or as part of a housing; a sample port; a microarray port; a lysis module; a purification module for containing a solid phase for binding of oligonucleotides; a thermocycling module for containing a polymerase chain reaction; a fragmentation module; and a microarray module for holding a microarray and a liquid in contact with the microarray. The apparatus is configured to be coupled to a device for: pumping a liquid through, in order, the lysis, purification, thermocycling, fragmentation, and microarray modules; sonicating any contents of the lysis module; thermocycling the thermocycling module to perform the polymerase chain reaction; heating the fragmentation module to fragment any oligonucleotides contained therein; circulating a fluid over the surface of the microarray; and performing one or more washing or staining steps on the microarray.

Abstract translation: 具有在壳体内或作为壳​​体的一部分的装置; 样品端口 一个微阵列港口 裂解模块; 用于含有寡核苷酸结合的固相的纯化模块; 用于含有聚合酶链式反应的热循环模块; 碎片模块; 以及用于保持微阵列和与微阵列接触的液体的微阵列模块。 该装置被配置为耦合到装置，用于：按顺序泵送液体裂解，纯化，热循环，碎裂和微阵列模块; 超声处理裂解模块的任何内容物; 热循环模块进行聚合酶链反应; 加热片段化模块以使其中所含的任何寡核苷酸片段化; 使流体在微阵列的表面上循环; 并在微阵列上进行一个或多个洗涤或染色步骤。

公开(公告)号：WO2007011412A2

公开(公告)日：2007-01-25

申请号：PCT/US2005/040196

申请日：2005-11-07

Applicant: THE GOVERNMENT OF THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF THE NAVY ,  AGAN, Brian, K. ,  HANSON, Eric, H. ,  JENKINS, Michael, J ,  LIN, Baochuan ,  OLSEN, Chris, C. ,  ROWLEY, Robb, K. ,  STENGER, David, A. ,  THACH, Dzung, C. ,  TIBBETTS, Clark, J. ,  WALTER, Elizabeth, A. ,  LIU, Jinny, Lin

Inventor： AGAN, Brian, K. ,  HANSON, Eric, H. ,  JENKINS, Michael, J ,  LIN, Baochuan ,  OLSEN, Chris, C. ,  ROWLEY, Robb, K. ,  STENGER, David, A. ,  THACH, Dzung, C. ,  TIBBETTS, Clark, J. ,  WALTER, Elizabeth, A. ,  LIU, Jinny, Lin

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/6806 ,  C12Q2600/158

Abstract: The present invention provides a specific set of gene expression markers from peripheral blood leukocytes that are indicative of a host response to exposure, response, and recovery infectious pathogen infections. The present invention further provides methods for identifying the specific set of gene expression markers, methods of monitoring disease progression and treatment of infectious pathogen infections, methods of prognosing the onset of an infectious pathogen infection, and methods of diagnosing an infectious pathogen infection and identifying the pathogen involved.

Abstract translation: 本发明提供了一组来自外周血白细胞的特定基因表达标记，其指示宿主对暴露，应答和恢复传染性病原体感染的应答。 本发明进一步提供了鉴定特定组基因表达标记物的方法，监测疾病进展和感染性病原体感染治疗的方法，预测感染性病原体感染发作的方法，以及诊断感染性病原体感染的方法和鉴定 涉及病原体。

公开(公告)号：WO2006138182A3

公开(公告)日：2009-04-23

申请号：PCT/US2006022622

申请日：2006-06-09

Applicant: US GOV SEC NAVY ,  MALANOSKI ANTHONY P ,  LIN BAOCHUAN ,  SCHNUR JOEL M ,  STENGER DAVID A

Inventor： MALANOSKI ANTHONY P ,  LIN BAOCHUAN ,  SCHNUR JOEL M ,  STENGER DAVID A

IPC: C12Q1/68 ,  C12P19/34 ,  G06F17/11

CPC classification number: G06F19/22 ,  G06F19/18 ,  G06F19/20 ,  Y10S707/99936

Abstract: A method of: submitting reference sequences to a taxonomic database to produce taxonomic results; and reporting a taxonomic identification based on the taxonomic results. The reference sequences are the output of genetic database queries that return a score for each reference sequence. A method for processing a biological sequence obtained from an assay by: converting base calls located in a predetermined list of positions within the biological sequence to N; and determining the ratio of single nucleotide polymorphisms in the biological sequence relative to a reference sequence. Each entry in the predetermined list of positions represents the capability of a substance hybridizing to a microarray used to generate the biological sequence. The substance is not the nucleic acid of a target pathogen.

Abstract translation: 一种方法：将参考序列提交给分类数据库以产生分类结果; 并根据分类结果报告分类鉴定。 参考序列是返回每个参考序列得分的遗传数据库查询的输出。 一种用于处理通过以下方式获得的生物学序列的方法：将位于所述生物学序列内的预定位置列表中的碱基呼叫转化为N; 并确定生物序列中单核苷酸多态性相对于参考序列的比例。 预定位置列表中的每个条目表示与用于产生生物序列的微阵列杂交的物质的能力。 该物质不是目标病原体的核酸。