METHOD FOR COMBATING ATTACK AND SPREAD OF FUNGAL PATHOGENS IN PLANTS
    11.
    发明申请
    METHOD FOR COMBATING ATTACK AND SPREAD OF FUNGAL PATHOGENS IN PLANTS 审中-公开
    用于组合真菌病原体在植物中的攻击和扩散的方法

    公开(公告)号:WO0167865A3

    公开(公告)日:2002-01-10

    申请号:PCT/EP0102725

    申请日:2001-03-12

    Abstract: The present invention concerns a method for introducing and/or improving plant resistance to the attack and/or spread of fungal pathogens. The method comprises applying at least one anti-fungal compound to a plant or plant part that has been genetically modified using recombinant DNA technology. The method results in a synergistic effect, as determined using Colby's formula, between the anti-fungal compound and the genetically modified plant or plant part which leads to improved resistance relative to control plants.

    Abstract translation: 本发明涉及一种用于引入和/或改善植物对真菌病原体的攻击和/或扩散的抗性的方法。 该方法包括使用重组DNA技术将至少一种抗真菌化合物施用于已被遗传修饰的植物或植物部分。 该方法使用Colby公式确定了抗真菌化合物与转基因植物或植物部分之间的协同效应,这导致相对于对照植物的改善的抗性。

    PATHOGEN-INDUCIBLE PROMOTER
    12.
    发明申请
    PATHOGEN-INDUCIBLE PROMOTER 审中-公开
    致病诱导剂

    公开(公告)号:WO9950428A3

    公开(公告)日:1999-12-23

    申请号:PCT/EP9902178

    申请日:1999-03-25

    CPC classification number: C12N15/8237

    Abstract: This invention describes pathogen-inducible promoters which normally drive expression of plant hexose oxidases, especially those which can be isolated from Helianthus annuus and Lactuca sativa, more specifically those promoters which naturally are the regulatory regions driving expression of the hexose oxidase MS59 and WL64, respectively. Also claimed are chimeric constructs where these pathogen-inducible promoters drive expression of antipathogenic proteins or of proteins which can elicit a hypersensitive response.

    Abstract translation: 本发明描述了通常驱动植物己糖氧化酶表达的病原体诱导型启动子,特别是可从向日葵和莴苣中分离的那些,更具体地说,那些分别驱动己糖氧化酶MS59和WL64表达的调控区的那些启动子 。 还要求的是嵌合构建体,其中这些病原体诱导型启动子驱动抗致病性蛋白质或可引发过敏反应的蛋白质的表达。

    ANTIFUNGAL PROTEINS, DNA CODING THEREFORE, AND HOSTS INCORPORATING SAME
    19.
    发明申请
    ANTIFUNGAL PROTEINS, DNA CODING THEREFORE, AND HOSTS INCORPORATING SAME 审中-公开
    抗真菌蛋白,其编码的DNA和与其相同的宿主

    公开(公告)号:WO1998013478A2

    公开(公告)日:1998-04-02

    申请号:PCT/EP1997004923

    申请日:1997-09-04

    Abstract: The present invention provides an isolated protein obtainable from a plant source which has antifungal activity, specifically anti-Phytophthora activity and/or anti-Pythium activity and a molecular weight of about 55-65 kDa as judged by SDS PAGE-electrophoresis, an isolated DNA sequence comprising an open reading frame capable of encoding a protein according to the invention, preferably characterised in that it comprises an open reading frame which is capable of encoding a protein depicted in SEQ ID NO. 16, SEQ ID NO. 57, SEQ ID NO. 70, SEQ ID NO. 72 or SEQ ID NO. 74 or muteins thereof, and DNA capable of hybridising therewith under stringent conditions. The invention further comprises plants incorporating chimeric DNA capable of encoding a protein according to the invention, and wherein the protein is expressed. Also shown is the carbohydrate and preferably hexose oxidating activity of said protein. Also methods are provided for combating fungi, especially Phytophthora and Pythium species, using a protein or a host cell capable of producing the protein.

    Abstract translation: 本发明提供从植物来源获得的分离的蛋白质,其具有抗真菌活性,特别是抗疫苗活性和/或抗 - 腐霉活性,并且通过SDS PAGE-电泳判断为约55-65kDa的分子量,分离的DNA 其包含能够编码本发明的蛋白质的开放阅读框,其特征在于,其包含能够编码SEQ ID NO:1所示蛋白质的开放阅读框。 16,SEQ ID NO: 57,SEQ ID NO: 70,SEQ ID NO: 72或SEQ ID NO。 74或突变蛋白,以及能够在严格条件下与其杂交的DNA。 本发明还包括含有能够编码本发明的蛋白质的嵌合DNA的植物,并且其中所述蛋白质被表达。 还显示了所述蛋白质的碳水化合物和优选己糖氧化活性。 还提供了使用能够产生蛋白质的蛋白质或宿主细胞来对抗真菌,特别是疫霉属(Phytophthora)和腐霉属(Pythium)物种的方法。

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