Abstract:
PURPOSE: A manufacturing method of a carrier for the islet cell transplantation using atelocollagen, and an artificial pancreas manufactured using the same are provided to be able to efficiently incubate islet cells and provide a carrier for the islet cell transplantation with an excellent stability and an artificial pancreas by using cationized atelocollagen. CONSTITUTION: A manufacturing method of a carrier for the islet cell transplantation comprises the following steps: a cationized atelocollagen solution and an alginate solution are mixed; islet cells are mixed in the mixed solution of the cationized atelocollagen solution and the alginate solution; an islet cell complex, which is surrounded by the mixed solution of the cationized atelocollagen solution and the alginate solution and mixed with islet cells, is formed; an islet cell complex, which is surrounded by the mixed solution of the cationized atelocollagen solution and the alginate solution and mixed with islet cells, is deposited in a chelating agent to produce a cationized atelocollagen/alginate bead in which an islet cell is accommodated inside. [Reference numerals] (AA) Number of langerhans islet cells; (BB) 1 day; (CC) 3 week; (DD) 8 week
Abstract:
PURPOSE: A method for culturing islet cells using atelocollagen is provided to effectively culture islet cells using a cationized atelocollagen or crosslinked atelocollagen support which is acquired by ionizing high purity atelocollagen. CONSTITUTION: A method for culturing islet cells using atelocollagen comprises the steps of preparing a cationized atelocollagen solution; applying the cationized atelocollagen solution on a culture container and drying to form a cationized atelocollagen support; inoculating islet cells to the cationized atelocollagen solution; and culturing the inoculated islet cells in the cationized atelocollagen solution or on the cationized atelocollagen support. [Reference numerals] (AA) Number of islet cells; (BB) 1 day; (CC) 3 weeks; (DD) 8 weeks
Abstract:
본 발명은 고순도의 아텔로콜라겐을 이온화시키는 과정을 통하여 양이온화 아텔로콜라겐을 제조한 후 이를 이용하여 췌도세포를 배양하는 방법 및 췌도세포 이식용 담체를 제조하는 방법 그리고 이를 이용하여 제조된 인공췌장을 제공한다.본 발명에 따르면 고순도의 아텔로콜라겐을 이온화시키는 과정을 통하여 수득된 양이온화 아텔로콜라겐 또는 가교화된 아텔로콜라겐 지지체를 이용하여 췌도세포의 배양시 췌도세포의 생존율 및/또는 글루코오스 의존적인 인슐린 분비량을 높일 수 있고, 양이온화 아텔로콜라겐과 알지네이트를 포함하는 안정성이 우수한 췌도세포 이식용 담체를 제공함으로써 배양 및 이식된 췌도세포의 생존율을 높이면서 동시에 글루코오스 의존적인 인슐린 분비량을 높일 수 있는 장점이 있다.