公开(公告)号：KR1020140091140A

公开(公告)日：2014-07-21

申请号：KR1020130002237

申请日：2013-01-08

Applicant: 한국교통대학교산학협력단

Inventor： 이상은 ,  조태민 ,  이향렬 ,  정경환

IPC: C12N9/38 ,  C12P19/44 ,  A61K8/60

CPC classification number: C12N9/2471 ,  A61K8/602 ,  A61K2800/524 ,  C12N15/74 ,  C12P19/14 ,  C12P19/46 ,  C12Y302/01023

Abstract: The present invention relates to a preparation method of chlorphenesin galactoside comprising the steps of: preparing a reactant consisting of chlorphenesin and lactose; mixing Escherichia coli including β-galactosidase to the reactant and making the Escherichia coli react with the reactant to obtain a reaction product; and obtaining chlorphenesin galactoside from the reaction product.

Abstract translation: 本发明涉及一种氯苯衍生物半乳糖苷的制备方法，包括以下步骤：制备由chlorphenesin和乳糖组成的反应物; 将包含β-半乳糖苷酶的大肠杆菌与反应物混合，使大肠杆菌与反应物反应，得到反应产物; 并从反应产物中获得氯苯辛素半乳糖苷。

公开(公告)号：KR1020080040844A

公开(公告)日：2008-05-09

申请号：KR1020060108717

申请日：2006-11-06

Applicant: 한국교통대학교산학협력단

Inventor： 정경환 ,  김현주

IPC: G01N33/48

Abstract: A method for measuring elastase inhibition activity is provided to screen, identify and quantify an elastase inhibitor in a sample through a simple method of putting an agar medium under constant temperature condition without requiring a separate measuring instrument. A method for measuring elastase inhibition activity comprises the steps of: (a) preparing a solid culture medium using agar medium including 0.01-1.0 wt.% of Elastin-Congo red; (b) after adding a sample which is subject to elastase inhibition activity measurement to the solid culture medium and putting it in an incubator under constant temperature condition of 30-42 deg.C for 3-72 hours, measuring a diameter of halo shown on the culture medium.

Abstract translation: 提供了一种测量弹性蛋白酶抑制活性的方法，通过简单的方法将琼脂培养基置于恒温条件下筛选，鉴定和定量样品中的弹性蛋白酶抑制剂，而不需要单独的测量仪器。 用于测量弹性蛋白酶抑制活性的方法包括以下步骤：（a）使用包含0.01-1.0重量％弹性蛋白 - 刚果红的琼脂培养基制备固体培养基; （b）在将固定培养基添加进行弹性蛋白酶抑制活性测定的样品后，在30-42℃的恒温条件下放入培养箱中3-72小时后，测定在 培养基。

公开(公告)号：KR1020100027437A

公开(公告)日：2010-03-11

申请号：KR1020080086355

申请日：2008-09-02

Applicant: 한국교통대학교산학협력단

Inventor： 정경환

IPC: C12N15/63 ,  C12N15/09

Abstract: PURPOSE: A method for enhancing activity of a target protein expressed in inclusion body type is provided to enhance activity of the inclusion body of target protein for therapy, research, and industry. CONSTITUTION: An inclusion body of a target protein enhances the activity by treating catabolite repressor or inducer analog in a microorganism which is transformed with arabinose-inducible expression vector derived from arabinose operon having a nucleic acid encoding the target protein. An inhibitor of metabolic product is methyl α-D-glucopyranoside. The inducer analog is D-fucose. The promoter of the expression vector is araBAD. The microorganism is E.coli, MC1061.

Abstract translation: 目的：提供一种增强包涵体型表达的靶蛋白活性的方法，以增强靶蛋白包涵体在治疗，研究和工业中的活性。 构成：靶蛋白的包涵体通过在由具有编码靶蛋白的核酸的阿拉伯糖操纵子的阿拉伯糖诱导型表达载体转化的微生物中处理分解代谢阻抑物或诱导物类似物来增强活性。 代谢产物抑制剂是甲基α-D-吡喃葡萄糖苷。 诱导剂类似物是D-岩藻糖。 表达载体的启动子是araBAD。 微生物是大肠杆菌，MC1061。