公开(公告)号：KR1020090020834A

公开(公告)日：2009-02-27

申请号：KR1020070085431

申请日：2007-08-24

Applicant: (주)아모레퍼시픽

Inventor： 안수선 ,  김서영 ,  이은영 ,  서병휘 ,  문성준

IPC: G01N33/48 ,  G01N33/53

Abstract: An in vitro skin sensitization test method is provided to rapidly analyze the skin sensitization-inducing possibility of material through the cell derived from the human body without use of animal, and classify and grade the skin sensitization-inducing possibility, thereby easily finding out the procedure required for the following process. The in vitro skin sensitization test method comprises the steps of: determining the treatment concentration of the estimation target material for the skin sensitization-inducing test; contacting the determined concentration of the estimation target material with the human origin cell, and culturing the cell; detecting CD86, CD54 and CD83 as costimulatory molecule, expressed from the cultured human origin cell; indexing the detected costimulatory molecules; grading and classifying the indexed detection amount; and determining the estimation target material as the skin sensitization-inducing material when the classified detection amount is the risk grade.

Abstract translation: 提供体外皮肤致敏试验方法，以快速分析通过源自人体的细胞的材料的皮肤敏化诱导可能性，而不使用动物，并分类和分级皮肤致敏诱导的可能性，从而容易地找出程序 需要以下过程。 体外皮肤致敏试验方法包括以下步骤：确定用于皮肤致敏诱导试验的估计目标材料的处理浓度; 将所测定的所述目标物质浓度与所述人源细胞接触，培养所述细胞; 从培养的人源细胞中检测CD86，CD54和CD83作为共刺激分子; 索引检测到的共刺激分子; 索引检测量分级和分类; 以及当所述分类检测量为风险等级时，将所述估计目标材料确定为所述皮肤致敏诱导材料。