公开(公告)号：KR1020110135724A

公开(公告)日：2011-12-19

申请号：KR1020100055608

申请日：2010-06-11

Applicant: 대한민국(농촌진흥청장) ,  가톨릭대학교 산학협력단

Inventor： 변승준 ,  전익수 ,  김성우 ,  김경운 ,  김점순 ,  류재규 ,  박수봉 ,  김태윤

IPC: C12N15/85 ,  C12N15/12 ,  A01K67/027

Abstract: PURPOSE: A transgenic chicken with human SOD-3 gene is provided to induce human SOD-3 gene and to produce useful protein for human body. CONSTITUTION: A recombinant expression vector, pLenti6/OVA-SOD(KACC 95104P), contains a gene of sequence number 1 with a promoter of chiken ovalbumin and human SOD-3 gene. The recombinant expression vector is prepared by introducing the gene of sequence number 1 into pLenti6/TR vector. The recombinant expression vector contains a sequence of sequence number 2. A transgenic chicken which is able to express the human SOD-3 gene is produced by transforming with the recombinant expression vector.

Abstract translation: 目的：提供具有人SOD-3基因的转基因鸡，以诱导人类SOD-3基因并为人体产生有用的蛋白质。 构成：重组表达载体pLenti6 / OVA-SOD（KACC 95104P）含有序列号1的基因，具有chiken卵白蛋白和人SOD-3基因的启动子。 通过将序列号1的基因导入pLenti6 / TR载体来制备重组表达载体。 重组表达载体含有序列号2的序列。能够表达人SOD-3基因的转基因鸡通过用重组表达载体转化产生。

公开(公告)号：KR1020110057365A

公开(公告)日：2011-06-01

申请号：KR1020090113734

申请日：2009-11-24

Applicant: 대한민국(농촌진흥청장)

Inventor： 변승준 ,  전익수 ,  김성우 ,  김경운 ,  김점순 ,  류재규 ,  박수봉

IPC: C12N15/867 ,  C12N15/12 ,  A01K67/027

Abstract: PURPOSE: A method for producing a transformed chicken containing human lactoferrin gene is provided to effectively produce a target proteins. CONSTITUTION: A lentivirus vector contains a promoter region in which human lactoferrin gene and ovalbumin gene are combined together. A recombinant lentivirus is prepared using the lentivirus vector in virus packaging cell, 293T cells. A method for producing a transformed chicken with human lactoferrin gene: a step of analyzing semen from transformed roosters and selecting semen containing human lactoferrin gene; and a step of fertilizing semen with hen and hatching.

Abstract translation: 目的：提供一种生产含有人乳铁蛋白基因的转化鸡的方法，以有效地产生靶蛋白。 构成：慢病毒载体含有人乳铁蛋白基因和卵清蛋白基因组合在一起的启动子区域。 使用病毒包装细胞293T细胞中的慢病毒载体制备重组慢病毒。 用人乳铁蛋白基因生产转化鸡的方法：从转化的雄鸡分析精液并选择含有人乳铁蛋白基因的精液的步骤; 并用母鸡和孵化器施肥精液的步骤。