公开(公告)号：KR1020140081512A

公开(公告)日：2014-07-01

申请号：KR1020120151353

申请日：2012-12-21

Applicant: 대한민국(농촌진흥청장)

Inventor： 이봉춘 ,  강미형 ,  노태환 ,  강인정 ,  신동범 ,  한성숙 ,  강항원

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q2537/143 ,  C12Q2563/113

Abstract: The present invention relates to a composition including a primer pair specific to bacterial grain rot, a primer pair specific to bacterial brown streak and a primer pair specific to bacterial leaf blight for diagnosing rice bacterial diseases; a primer pair for diagnosing rice bacterial diseases; a kit including the primer pair for diagnosing rice bacterial diseases; and a method for simultaneous diagnosis of bacterial grain rot, bacterial brown streak and bacterial leaf blight, including a determination step by performing multiple PCRs using DNA separated from rice as a template using the primer pairs. The diagnosis method using the kit for simultaneous diagnosis of major three rice bacterial diseases of the present invention enables simultaneous diagnosis of a complex infection contagion plant such that time and efforts can be reduced, rapid early diagnosis of rice bacterial diseases can be possible, and thus the method can contribute to establishment of a general management system of rice bacterial diseases and maintain high quality seeds.

Abstract translation: 本发明涉及一种组合物，其特征在于，包括细菌颗粒腐烂特异性的引物对，细菌褐色条纹特异的引物对和用于诊断水稻细菌病的细菌性叶枯病特异性引物对; 用于诊断水稻细菌性疾病的引物对; 包括用于诊断水稻细菌性疾病的引物对的试剂盒; 以及用于同时诊断细菌颗粒腐烂，细菌褐色条纹和细菌性叶枯病的方法，包括使用引物对使用从水稻分离的DNA作为模板进行多个PCR的测定步骤。 使用本发明的三大水稻细菌性疾病同时诊断试剂盒的诊断方法能够同时诊断复杂的感染传染病植物，从而可以减少时间和努力，可以快速早期诊断水稻细菌病，因此 该方法可有助于建立水稻细菌性疾病综合管理体系，保持优质种子。