Abstract:
PURPOSE: A pretreatment system and a method for manufacturing substrate for ethanol fermentation from cellulose biomass are provided to efficiently separate carbohydrate for ethanol generation from cellulose biomass by extruding reaction products in highly expanded state to a low temperature and low pressure separation tank. CONSTITUTION: A pretreatment system and a method for manufacturing substrate for ethanol fermentation comprises a reactor(10), a separation tank(11), and a micro nozzle(12). In the reactor, acid or alkaline solution, and carbon dioxide are injected to the cellulose-based biomass powder sample, and reaction under high temperature and high pressure is reacted. Inside the separation tank is maintained to low temperature and low pressure. The micro nozzle can separate carbohydrate from the cellulose-based biomass by spraying reactant of high temperature and high pressure generated from the reactor into the separation tank.
Abstract:
본발명은동시당화발효효능이우수한고온내성신규균주및 이를이용한바이오에탄올생산방법에관한것이다. 본발명은사카로마이세스세레비제()에결손돌연변이를유발하여고온내열성균주를분리하였으며분리된균주는 40 내지 45℃의고온에서우수한성장률과에탄올생성율을나타내므로, 이를고온에서의동시당화발효법을이용한바이오에탄올생산에적용할수 있다.
Abstract:
The present invention relates to xylanase of Bacillus pumilus H10-1 strain. More specifically, the xylanase which is produced by the Bacillus pumilus H10-1 strain isolated from excretion of ceratotherium simum has enhanced cellulolytic activity, thereby can be used for cellulosic biomass saccharification. By using the xylanase as an enzyme for feed addition, the efficiency for domestic animals to use nutrition can be increased.
Abstract:
The present invention relates to an SCAR marker for distinguishing Miscanthus sacchariflorus which is a high yield perennial energy crop, which is able to quickly distinguish Miscanthus sacchariflorus from other Miscanthus without the influence in a culture environment or growth time. One side of the present invention provides a method for distinguishing Miscanthus sacchariflorus comprising the steps of: isolating genomic DNA from Miscanthus; amplifying a target sequence by RAPD-PCR using the isolated genomic DNA as a template and a primer of sequence number 1; and identifying the position of bands of the amplified DNA by electroporation.
Abstract:
PURPOSE: Miscanthus sacchariflorus named Wooram is provided to function as a raw material for environmentally-friendly construction materials and high density pellet fuel. CONSTITUTION: Miscanthus sacchariflorus, which is named Wooram, is tetraploid Miscanthus sacchariflorus Benth. and has 76 chromosomes. In the polymorphism analyzing process with a N8018 random primer, one band exists at 1,800bp and has morphological features as follows. The lower part of a stem is dark violet, and the inside of the stem is hollow. The number of sprouts is 51± 8/m^2. The length of the stem is 313± 32cm. The diameter of a ground stem is 9.7±1.0mm. The quantity of dried plants is 19.6±1.5 ton/ha. The miscanthus sacchariflorus is cultivated using the callus of the miscanthus sacchariflorus (Accession number: KCTC12088BP).