Abstract:
본 발명은 클로버에 대한 제초활성을 갖는 균주인 스클레로티니아 트리폴리오럼( Sclerotinia trifoliorum ) BWC98-105 및 이를 이용한 클로버 제초용 균사체 현탁액 제조방법에 관한 것으로, 보다 상세하게는 클로버에 대하여 기주특이적으로 기생하고 제초활성을 갖는 균주, 스클레로티니아 트리폴리오럼 BWC98-105를 선발하고 이 균주를 배양하여 클로버 방제에 효과적인 균사체 현탁액을 제조하는 방법에 관한 것이다 본 발명의 균주를 이용하여 제조된 균사체 현탁액을 포장에 처리하면 지속적으로 클로버 지하경 및 줄기 등 지상부 식물체가 고사될 뿐만 아니라 다음해 클로버 발생을 억제할 수 있고 잡초를 생물학적으로 방제시켜 유기합성 제초제 사용을 줄임으로써 환경 오염을 경감시킬 수 있다.
Abstract:
PURPOSE: Provided is an isolation method of a secondary metabolic product with herbicidal effect on the white clover(Trifolium repens), which is released from culture extract of isolate BWC98-105(Sclerotinia sp.), thereby withering the subterranean stem and stem of the white clover continuously, and inhibiting the generation thereof consequently to reduce the consumption of agricultural chemicals. CONSTITUTION: An isolation method of a secondary metabolic product with herbicidal effect on the white clover(Trifolium repens) by using BWC98-105(Sclerotinia sp.) comprises the steps of: culturing BWC98-105 strain in PD broth medium for 7 days, followed by systematic extraction with an organic solvent; fractioning the extraction with butanol and ethylacetate; separating the butanol and ethylacetate fractions using the column filled with reverse phase silica gel(C18); and analyzing the separated fractions by HPLC, wherein a second metabolic product having herbicidal effect on the white clover(Trifolium repens) is produced at retention time intervals of 4.13 minute.
Abstract:
PURPOSE: Provided is a method for manufacturing mycelia suspension having herbicidal effect on white clover(trifolium repens) by using BWC98-105(Sclerotinia sp.), thereby withering the subterranean stem and stem of the white clover continuously, and inhibiting the generation thereof. CONSTITUTION: A method for manufacturing mycelia suspension having herbicidal effect on white clover(trifolium repens) by using BWC98-105(Sclerotinia sp.) comprises the steps of: culturing BWC98-105 strain in PD broth medium, at 28 deg.C and 150rpm; forming semi-solid mycelia; growing the mycelia to aerial mycelia through static culture and forming sclerotium thereon; and forming mycelia suspension by pulverizing the mycelia of BWC98-105 strain.