Abstract:
PURPOSE: A microsatellite marker for distinguishing lineage of red mold pathogen and a method for distinguishing lineage of red mold pathogen using the same are provided to quickly and accurately distinguish lineage of a main contamination source of complex red mold pathogen from a grain sample by analyzing PCR using FgmsAAG93. CONSTITUTION: A primer, FgmsAAG93, of sequence numbers 1 and 2 is used for specifically amplifying a microsatellite of red mold to distinguish lineage of a main contamination source among red mold pathogens. A method for distinguishing lineage of F. graminearum comprises: a step of amplifying a gene using FgmsAAG93; and a step of identifying a microsatellite site of the amplified gene. A kit for distinguishing and diagnosing lineage of the red mold pathogens contains the primer and a reagent for amplification.
Abstract:
PURPOSE: A primer pair for detecting deoxynivalenol(DON)-producing Gibberella zeae and a kit using the same are provided to quickly and accurately diagnose DON. CONSTITUTION: A primer pair for detecting DON-producing Gibberella zeae is denoted by sequence numbers 1 and 2. The Gibberella zeae is Fusarium graminearum. A kit for detecting the Gibberella zeae contains the primer pair. A method for detecting the Gibberella zeae using the primer pair comprises: a step of isolating RNA from a sample of Oryza sativa, barley, and wheat; a step of amplifying a target sequence by RT-PCR; and a step of detecting a PCR product.
Abstract:
본발명은후자리움속검출용프라이머및 이를이용하여후자리움속을검출하는방법에관한것이다. 본발명에따른프라이머쌍을이용하면, 후자리움()속에속하는종(species) 중 B형트라이코쎄신생성능을갖는붉은곰팡이를형성하는후자리움속및 이의근연종만을특이적으로우수한효율로단시간내에검출할수 있다.
Abstract:
PURPOSE: A microsatellite marker for distinguishing foreign species of Fusarium graminearum and a distinguishing method using the same are provided to quickly and accurately distinguish species and toxin of a red mold pathogen. CONSTITUTION: A primer, FgmsTG49, of sequence numbers 1 and 2 is used for specifically amplifying a microsatellite of red mold pathogen for distinguishing foreign strain, domestic strain, and mixed strain thereof among Fusarium graminearum. A method for distinguishing the foreign species among Fusarium graminearum comprises: a step of amplifying a gene using the primer; and a step of identifying the microsatellite of the amplified gene. A kit for distinguishing and diagnosing the foreign species contains the primer and a reagent for amplification.
Abstract:
PURPOSE: A microsatellite marker for distinguishing species according to the type of toxin generated from red mold pathogen and a method for distinguishing species of pathogen using the same are provided to quickly and accurately distinguish main species and toxin by analyzing PCR using FgmsGT28. CONSTITUTION: A primer, FgmsGT28, of sequence numbers 1 and 2 is able to specifically amplify a microsatellite of red mold pathogen to distinguish Fusarium graminearum which produces deoxynivalenol(DON) and Fusarium asiaticum which produces nivalenol(NIV) by PCR(Polymerization Chain Reaction). A method for distinguishing F. graminearum and F. asiaticum comprises: a step of amplifying a gene using the primer by PCR; and a step of identifying a microsatellite site of the amplified gene. A kit for diagnosing and distinguishing species of red mold pathogen contains the primer and a reagent for amplification.