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公开(公告)号：KR1020120022387A
公开(公告)日：2012-03-12
申请号：KR1020100085955
申请日：2010-09-02
Applicant: 서울대학교산학협력단
IPC: C12Q1/68 , C12N15/11 , G01N33/576
CPC classification number: C12Q1/6883 , G01N33/5761 , G01N33/6854
Abstract: PURPOSE: A kit for diagnosing HBe antigen negative chronic hepatitis type B and liver diseases is provided to effectively diagnose liver diseases. CONSTITUTION: A kit for diagnosing HBe antigen negative chronic hepatitis type B contains a primer having bases of seqeunce numbers 1-4. The kit comprises: a DNA probe having base sequences of sequence numbers 17-24 binding to a gene encoding F38S core antigen protein; a linker which contains oligo(dT)15, -(CH2)6-, and amine group in order; and a solid substrate in which an aldehyde group is attached on the surface and which has aldehyde group on the surface linked to an amine group by Schiff's base reaction.
Abstract translation: 目的：提供诊断HBe抗原阴性慢性乙型肝炎和肝脏疾病的试剂盒，以有效诊断肝脏疾病。 构成：用于诊断HBe抗原阴性慢性乙型肝炎B的试剂盒含有一个具有数字1-4的碱基的引物。 试剂盒包括：具有与编码F38S核心抗原蛋白的基因结合的序列号17-24的碱基序列的DNA探针; 含有寡聚（dT）15， - （CH 2）6 - 和胺基的接头; 以及固体基质，其中醛基通过席夫氏碱反应连接在胺基上的表面上并具有醛基。
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公开(公告)号：KR101662917B1
公开(公告)日：2016-10-06
申请号：KR1020147005184
申请日：2012-11-08
Applicant: 서울대학교산학협력단
CPC classification number: C12N15/85 , C12N2730/10122 , C12N2830/60
Abstract: 본발명은서열목록제1서열, 서열목록제2서열, 서열목록제3서열, 서열목록제4서열, 서열목록제5서열, 서열목록제6서열, 서열목록제7서열, 서열목록제8서열, 서열목록제9서열, 서열목록제10서열, 서열목록제11서열, 서열목록제12서열, 서열목록제13서열및 서열목록제14서열의폴리뉴클레오타이드로구성된군으로부터선택되는진핵세포용 cis-작용엘리먼트에관한것이다. 본발명은cis-작용엘리먼트및 진핵세포에서작동하는(operable) 프로모터를포함하는동물세포용발현벡터를제공한다. 본발명의벡터는재조합단백질을동물세포에서효과적으로발현시킨다. 본발명의벡터및 이를이용한재조합단백질의제조방법은의약품의산업적대량생산에매우유용하게사용될수 있다.
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公开(公告)号：KR1020140044924A
公开(公告)日：2014-04-15
申请号：KR1020147005184
申请日：2012-11-08
Applicant: 서울대학교산학협력단
CPC classification number: C12N15/85 , C12N2730/10122 , C12N2830/60
Abstract: The present invention relates to a cis-regulatory element for an eukaryotic cell. The cis-regulatory element is selected from the group consisting of polynucleotides of sequence numbers 1-14. The present invention provides an animal cell expression vector including the cis-regulatory element and a promoter that is operable in an eukaryotic cell. The vector of the present invention effectively expresses recombinant proteins in an animal cell. The vector of and a method for producing a recombinant protein using same of the present invention can be very effectively used for the industrial mass production of medicine and medical supplies.
Abstract translation: 本发明涉及一种用于真核细胞的顺式调节元件。 顺式调节元件选自序列号1-14的多核苷酸。 本发明提供了包含顺式调节元件和可在真核细胞中操作的启动子的动物细胞表达载体。 本发明的载体在动物细胞中有效表达重组蛋白。 使用本发明的载体和使用本发明的重组蛋白质的制备方法可以非常有效地用于医药和医疗用品的工业批量生产。
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Patent Agency Ranking14.发明授权
公开(公告)号：KR101382734B1
公开(公告)日：2014-04-08
申请号：KR1020120034313
申请日：2012-04-03
Applicant: 서울대학교산학협력단
Abstract: HBV의 W4P preS1 변이 단백질을 코딩하는 뉴클레오티드 서열을 포함하는 HBV 유전체, 상기 HBV 유전체를 포함하는 발현시스템, 상기 HBV 유전체로부터 생성된 HBV 변이주 및 이를 이용한 HBV 감염 치료용 후보 물질의 스크리닝 방법이 제시되어 있다. 본 발명의 HBV 유전체는 외피 항원의 합성을 유도하고 유전체 복제 및 비리온 생성능이 높아, B형 간염 바이러스를 타겟으로 하는 HBV 감염 치료용 후보 물질을 스크리닝하는데 유용하며, 기타 HBV 비리온 형성 및 복제와 관련된 간질환의 악화 유도 기전 발굴의 도구로 유용하게 사용될 수 있다.
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公开(公告)号：KR1020130075512A
公开(公告)日：2013-07-05
申请号：KR1020110143902
申请日：2011-12-27
Applicant: 서울대학교산학협력단
CPC classification number: C01B32/184 , B41J2/01 , B41M3/006
Abstract: PURPOSE: Micro-patterning of graphene using inkjet printing and applying the micro-patterns to a flexible thin film electrode are provided to freely form patterns have no limit to a form. CONSTITUTION: A method for forming a flexible graphene thin film electrode is as follows. A water-dispersible graphene oxide nanoparticle solution utilized as conductive ink for an inkjet printing is manufactured after dissolving oxidation graphite in the water by using an ultrasonic generator. Chemical properties of the surface of a polymer film are modulated by plasma-processing so that the water-dispersible graphene oxide nanoparticle solution forms precise patterns on the flexible polymer film. After manufacturing ink by adding an auxiliary solvent into the water-dispersible graphene oxide nanoparticle solution, the manufactured ink is injected into a printer head and discharged by an inkjet printer on the plasma-processed polymer film so that a graphene thin film is formed. The polymer film in which the graphene thin film is formed is arranged inside a vapor deposition reactor. A reduction reaction is processed on the surface of the polymer film by vaporizing a reducing agent for proper time under a proper temperature so that graphene sheet patterns are formed. The graphene sheet variously patterned on the polymer film is utilized as an electrode so that properties of the electrode such surface resistance and flexibility are analyzed. [Reference numerals] (AA) Water-dispersible graphene oxide nanoparticle solution; (BB) Nozzle; (CC) Plastic substrate; (DD) Front unit; (EE) Rear unit; (FF) Progressive direction; (GG) 27V DC
Abstract translation: 目的：使用喷墨印刷和将微图案应用于柔性薄膜电极的石墨烯的微图案化被提供以自由形成图案对形状没有限制。 构成：用于形成柔性石墨烯薄膜电极的方法如下。 通过使用超声波发生器将氧化石墨溶解在水中后，制造用作喷墨印刷用导电油墨的水分散性石墨烯氧化物纳米粒子溶液。 通过等离子体处理来调节聚合物膜表面的化学性质，使得水分散性石墨烯氧化物纳米颗粒溶液在柔性聚合物膜上形成精确的图案。 在通过向水分散性石墨烯氧化物纳米颗粒溶液中添加辅助溶剂制造油墨之后，将所制造的油墨注入打印机头中，并通过喷墨打印机在等离子体处理的聚合物膜上排出，从而形成石墨烯薄膜。 其中形成有石墨烯薄膜的聚合物膜布置在蒸镀反应器的内部。 通过在合适的温度下蒸发还原剂适当的时间，在聚合物膜的表面上进行还原反应，从而形成石墨烯片图案。 将在聚合物膜上各种图案化的石墨烯片用作电极，从而分析电极的表面电阻和柔韧性。 （附图标记）（AA）水分散性氧化石墨烯纳米颗粒溶液; （BB）喷嘴; （CC）塑料基材; （DD）前置单元; （EE）后置单元; （FF）进步方向; （GG）27V DC
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公开(公告)号：KR1020130112151A
公开(公告)日：2013-10-14
申请号：KR1020120034318
申请日：2012-04-03
Applicant: 서울대학교산학협력단
IPC: A01K67/027 , C12N15/36 , C12N15/85 , G01N33/15
CPC classification number: A01K67/027 , A01K2217/20 , C12N15/113 , C12N15/85 , G01N33/15
Abstract: PURPOSE: A transformed animal which reveals a preS1 W4P variation protein of a hepatitis B virus and a use thereof are provided to valuably use for investigation of pathogenesis of a liver disorder which is sex-specific as a liver-disordered animal model and to valuably use a liver disease medicine for screening. CONSTITUTION: A non-human transformed animal which has been through transformation with an HBV dielectric contains a W4P preS1 gene to reveal a W4P preS1 variation protein of hepatitis B virus (HBV). A dielectric HBV dielectric is an A type or a C type. The HBV dielectric has a base sequence from 1433 to 4674 among the base sequence of a sequence number 1. The HBV dielectric has a base sequence from 1448 to 4694 among the base sequence of a sequence number 2. The transformed non-human animal is a liver disease model which reveals over-expression of an HBs antigen and an HBe antigen protein.
Abstract translation: 目的：揭示乙型肝炎病毒的PreS1 W4P变异蛋白的转化动物及其用途，有助于调查作为肝脏无序动物模型的性别特异性的肝脏疾病的发病机制，并有价值地使用 一种用于筛查的肝病药物。 构成：已经通过HBV电介质转化的非人转化动物含有W4P preS1基因，以揭示乙型肝炎病毒（HBV）的W4P preS1变异蛋白。 电介质HBV电介质是A型或C型。 HBV电介质在序列号1的碱基序列中具有1433至4674的碱基序列。HBV电介质在序列号2的碱基序列中具有1448至4694的碱基序列。转化的非人动物是 揭示HBs抗原和HBe抗原蛋白过度表达的肝脏疾病模型。
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公开(公告)号：KR101248427B1
公开(公告)日：2013-03-28
申请号：KR1020100085955
申请日：2010-09-02
Applicant: 서울대학교산학협력단
IPC: C12Q1/68 , C12N15/11 , G01N33/576
Abstract: 본 발명은 B형 간염에서 유래된 간질환 환자에 특이적인 HBV로부터 유래된 변이된 코어 항원 유전자 또는 단백질을 검출하여, HBe 항원 음성 만성 B형 간염 및 이로부터 유래된 간질환을 진단할 수 있는 진단용 키트에 관한 것이다. 본 발명의 HBe 항원 음성 만성 B형 간염 진단용 키트를 사용할 경우, HBe 항원 음성 만성 B형 간염에 특이적인 변이된 HBV 코어 항원 유전자 또는 단백질을 용이하게 검출할 수 있으므로, 변이된 코어 항원 유전자 또는 단백질을 포함하는 HBV에 의하여 발병되는 HBe 항원 음성 만성 B형 간염 및 이로부터 유래된 간질환을 조기에 효과적으로 진단할 수 있을 것이다.
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公开(公告)号：KR1020120022382A
公开(公告)日：2012-03-12
申请号：KR1020100085942
申请日：2010-09-02
Applicant: 서울대학교산학협력단
IPC: C12Q1/68 , C12N15/11 , G01N33/576
CPC classification number: C12Q1/6883 , G01N33/5761 , G01N33/6854
Abstract: PURPOSE: A kit for diagnosing HBe antigen-negative chronic hepatitis type B and liver diseases is provided to easily detect HBV-specific mutated HBV S antigen gene or protein. CONSTITUTION: A kit for detecting HBe antigen-negative chronic hepatitis type B contains a primer of sequence numbers 1-4. The kit contains: a DNA probe having base sequences of sequence numbers 11-18, which specifically binds to a gene encoding 100th amino acid of L110I S antigen protein; a linker which contains oligo(dT)15, -(CH2)6-, and amine group in order and is connected to thymine region at 5' terminal of the DNA probe; and a solid substrate having aldehyde which is attached on the surface, in which an amine group of an electric linker is connected to the aldehyde group by Schiff's base reaction.
Abstract translation: 目的：提供用于诊断HBe抗原阴性慢性乙型肝炎和肝脏疾病的试剂盒，用于容易地检测HBV特异性突变HBV S抗原基因或蛋白质。 构成：用于检测HBe抗原阴性慢性乙型肝炎的试剂盒含有序列号1-4的引物。 试剂盒包含：具有序列号11-18的碱基序列的DNA探针，其具体结合编码L110I S抗原蛋白的第100个氨基酸的基因; 包含寡聚（dT）15， - （CH 2）6 - 和胺基的连接体，并连接到DNA探针的5'末端的胸腺嘧啶区; 和具有醛的固体基质，其上附着有表面，其中电接头的胺基通过席夫碱反应与醛基连接。
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